Báo cáo khoa học: Structures of Phanerochaete chrysosporium Cel7D in complex with product and inhibitors ppt

... needed. Binding of thio-linked substrate analogue GG-S-GG and cellobiose Crystals of Pc _Cel7D were soaked with a combination of cellobiose and the thio-linked sugar GG-S-GG, in hopes of obtaining a complex ... 2005 FEBS Cel7D ⁄ saccharide complex structures W. Ubhayasekera et al. Structures of Phanerochaete chrysosporium Cel7D in complex with product...

Ngày tải lên: 23/03/2014, 13:20

... sulfo- bromophthalein to rat and human ligandins: characteri- zation of a binding-site peptide. Biochim Biophys Acta 955, 296–300. 52 Gregus Z & Klaassen CD (1982) Role of ligandin as a binding protein and ... bilirubin, biliverdin, section B) and in Table 2 (K d of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin 3-glucoside, sections C an...

Ngày tải lên: 20/02/2014, 01:20

... determination of X-ray struc- tures of wild-type and C69A mutant TcRpiB, with and without bound substrate and inhibitors, allowed us to study in detail the interactions between the enzyme and bound ... end of the substrate points into a deep pocket in the enzyme. Moving along the ligand from the phosphate, O4 inter- acts with His102¢ and a water molecule that is in...

Ngày tải lên: 15/03/2014, 00:20

... strains of P. vulgaris, 39 strains of P. mirabilis, 24 strains of P. penneri, one strain of P. myxofaciens, one strain of P. hauseri and Providen- cia stuartii O18, only LPS of P. vulgaris OX2 and Fig. ... 1 : 8000 and 1 : 32 000, respectively) and inhibition of the reaction in both PIH and EIA. Again, the homologous LPS was a strong inhibitor (1–4 ng) whereas the in...

Ngày tải lên: 16/03/2014, 23:20

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the ... of cHSF2, mHSF2 and rHSF2 are associated with nuclear locali- zation within the developing neural tube [3,62,121]. In addition, mHSF2 is associated with DNA binding in the de...

Ngày tải lên: 18/02/2014, 04:20

... structure (A), N and C represent the N- and C-termini of the protein, and a and b represent the a helix and b strand, respectively. The side-chains of the mutated and parent amino acid residues ... side-chains of K89, residue 90 and residue 97 are shown. The dis- tances between the e-amino groups of K89 and K90, and between the e-amino group of K90 and the guanid...

Ngày tải lên: 18/02/2014, 13:20

... pheromones to elicit a mating response in cells of the opposite mating type. Cdc42 is involved in pheromone signal- ling that eventually results in G 1 arrest and the for- mation of a mating projection ... formation of a mating projection [28]. Furthermore, homologues of oxysterol- binding proteins, a family of proteins that regulate the synthesis and transport of sterol...

Ngày tải lên: 18/02/2014, 13:20

... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl; and the fourth domain ... the interactions occurring at the binding site, and to develop a tool that could assist further optimization of inhibitors, a molecular model of the methyltetra- hydrofolate-binding doma...

Ngày tải lên: 19/02/2014, 05:20

... Sequence and structure determinants of the nonenzymatic deamidation of asparagine and glutamine residues in proteins. Protein Eng. 4, 283–294. 36. Tyler-Cross, R. & Schirch, V. (1991) Effects of ... The observation of species of increasing mass spaced by 162 Da reflects the presence of glycoforms with an increasing number of hexose (probably mannose) units. Phosphoryla...

Ngày tải lên: 19/02/2014, 12:20

... proteins. Immunostaining of meiotic C. cinereus nuclei Immunostaining of meiotic C. cinereus nuclei was perfomed as described in previous reports [29–31] with minor modi- fications. C. cinereus ... m M 2-mercaptoethanol, and proteinase inhibitors [1 m M phenylmethylsulfonyl fluoride, 1 lgÆmL )1 leupeptin, 1 lgÆmL )1 pepstatin A, and Protease Inhibitor Cocktail (Roche)]. Aliquots...

Ngày tải lên: 20/02/2014, 11:20