Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt

... Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester Yan Zhou 1 , Barbara I. Chase 1 , Mark Whitmore 2 , ... RNA-activated protein kinase. Proc Natl Acad Sci USA 90, 232–236. 10 Gil J & Esteban M (2000) Induction of apoptosis by the dsRNA-dependent protein kinase (PKR): mechanism of action. Apoptosis 5, ... activation by...

Ngày tải lên: 23/03/2014, 13:20

... > HIS-OXI > HIS-PTI > < HIS-PTI < GST-MPK6 < HIS-OXI GST: lof lof - MPK3 MPK6 GST-MPKs . HIS: 72- 55- HIS-OXI1 > -72 -55 < HIS-OXI1 < HIS-PTI < GST-MAP K < HIS-OXI OXI1 A B Fig. ... [8,9]. The AGC kinases were named on the basis of their homology to the mammalian cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependen...

Ngày tải lên: 14/02/2014, 19:20

... pathways mediated by ERK, JNK, and p38 protein kinases. Science 298, 1911–1912. 18 Morrison DK & Davis RJ (2003) Regulation of MAP kinase signaling modules by scaffold proteins in mam- mals. ... mechanism by which such inhibitors effect their anti- tumor action. AG1478 is known to selectively inhibit EGFR-tyrosine kinase. In this study, we showed that AG1478 caused apoptosis...

Ngày tải lên: 18/02/2014, 13:20

... kinases, e.g. protein kinase C (PKC), small GTPases, e.g. ADP- ribosylation factor, RhoA and Ral, phosphatidylinositol 4,5-bisphosphate, and tyrosine kinases [2–4]. To date, two distinct isoforms ... polyacrylamide gel for immunoblot analysis. Immunoblot analysis Proteins were denatured by boiling for 5 min at 95 °Cin Laemmli sample buffer [19], separated by SDS/PAGE, and transferred...

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 29/03/2014, 08:20

... S, Shani G, Berrisi H, Eisenstein M & Kimchi A (2001) The pro-apoptotic function of death-associated protein kinase is controlled by a unique inhibitory autopho- sphorylation-based mechanism. J Biol ... DM (2004) A calmodulin- regulated protein kinase linked to neuron survival is a substrate for the calmodulin-regulated death-associated protein kinase. Biochemistry 43, 81...

Ngày tải lên: 29/03/2014, 08:20

... delamination is purely mechanical or facilitated by a biochemical mechanism is unknown. The exact location where a bleb is nucle- ated in a zone of elevated intracellular pressure could be determined by ... (ERM) family of actin–membrane linker proteins or by being chelated by myristoylated alanine-rich C kinase sub- strate [24]. Delamination from the actin cortex is observe...

Ngày tải lên: 29/03/2014, 08:20

... and apoptosis, the role of death-associated protein kinase (DAPK) in the regulation of autophagy is particularly interesting. DAPK is a serine ⁄ threonine kinase originally isolated by a genetic ... the situation is complicated by the fact that autophagy is tightly linked to other cellular death mechanisms, apoptosis and necrosis [16]. Furthermore, the interplay between auto...

Ngày tải lên: 29/03/2014, 08:20

... UK Introduction Death-associated protein kinase (DAPK) is a cal- cium ⁄ calmodulin-regulated serine ⁄ threonine protein kinase, located on chromosome 9q21.33, which is com- posed of several functional domains, including a kinase domain, ... 2009, revised 28 May 2009, accepted 17 June 2009) doi:10.1111/j.1742-4658.2009.07414.x Death-associated protein kinase (DAPK) is a pro-...

Ngày tải lên: 29/03/2014, 08:20

... the overexpressed protein was finally purified by SP ion-exchange chromatography [24]. The purity of the protein was verified by SDS/PAGE. Protein concentration was determined by the method of Lowry et ... grade. Purification of microtubular proteins (MTP), tubulin, MAP2, MAP2c and Tau proteins MTP was isolated from goat brain by two to four cycles of temperature-dependent assembly...

Ngày tải lên: 23/03/2014, 12:20