Báo cáo khoa học: Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity docx

... Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity Martin Willemoe ¨ s 1 , Anne ... K A for GTP and reduced GTP activation of CTP synthesis of the mutant enzymes are in agreement with a predicted interaction of bound GTP with lid L11 and indicate that the GTP a...

Ngày tải lên: 23/03/2014, 13:20

... guanylyl cyclase activity can be observed even in the absence of ligand. Again, in contrast to the significant loss of ST-mediated activation of guanylyl cyclase activity, 50% of the activity seen ... the V max of the guanylyl cyclase catalytic activity of GC-C with no change in the S 0.5 of the enzyme for its substrate, MgGTP [25]. There did not appear to...

Ngày tải lên: 08/03/2014, 08:20

... built from the X-ray structure of the C-terminal domain of Ure2p and integrates the finding that the N-terminal domain of Ure2p is flexible. An enlargement of the region of Ure2p involved in the interaction ... were observed in the presence of ATP. No change in the mobility of the Ure2p–Ssa1p com- plexes was detected in the presence of ATP. The stoichio...

Ngày tải lên: 15/03/2014, 00:20

... Apparently, the amount of HGF is not correlated with the activity of HGF, suggesting that HGF is latent in normal states, and is activated specifi- cally at the site of tissue injury. The mechanism of localized ... identification of the enzyme would be crucial to understanding the control of HGF action in vivo. The activity of this enzyme, however, was not high e...

Ngày tải lên: 15/03/2014, 11:20

... the logarithm of the molecular mass of the proteins in the y-axis and the elu- tion volumes (V e ) in the x-axis. Comparison of the elution volumes of the P. abyssi and E. coli ATCases with the standard ... consequence of the cooperativity toward aspartate and of the ordered binding of the substrates, first CP then aspartate [13]. These results raised the...

Ngày tải lên: 16/03/2014, 19:20

... vitro chaperone activity of MTB HSP 16.3 and aB-crystallin In Fig. 3, we observed the effects of different concentrations of MTB HSP 16.3 in the presence and absence of ATP on the aggregation of CS. In the ... participate in the refolding of denatured proteins in vitro, in an ATP-dependent manner [21,22]. While the molecular chaperone effects of the sHsp do not r...

Ngày tải lên: 23/03/2014, 21:21

... content of the samples was determined with the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA) and the luciferase activity was normalized to the protein content of the wells. The activities of ... unable to bind to the ECD of the receptor protein. Analysis of the data revealed that WFIKKN1 and WFIKKN2 caused a 50% decrease in the rate of association of G...

Ngày tải lên: 06/03/2014, 02:20

... maintaining some of the vital functions of the body, but the same technology does not necessarily allow us to heal underlying disease processes [2]. An unintended side effect of modern technological ... animation; they are not alive in the sense the we enjoy life but neither are they able to die as long as nutrition, hydration, ventilation, and perfusion are assured. In many c...

Ngày tải lên: 25/10/2012, 10:45

... embryo- genesis, all the evidence suggests that sex determination might disobey the conventional rules of evolutionary conservation. The common picture emerging here is that the genes at the top of the cascade ... result, the common emerg- ing picture is that the genes at the top of the cascade are not conserved, whereas the downstream genes have homologues in a mu...

Ngày tải lên: 14/02/2014, 19:20

... containing the mutations of the miR-23a binding site (Fig. 4A). It was shown that miR-23a ASO or pcDNA3 ⁄ pri-23a did not affect the intensity of EGFP fluorescence in the vector bearing the mutant of the ... confirmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulate their targets by direct clea...

Ngày tải lên: 18/02/2014, 04:20