... interaction,because of its exposure to solvent upon the disso-Y232W F279WY232A F279AWTD231W G236WS233W1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 joint molecule (jm)nicked circular ... prepared the Rad51-Y232A and Rad51-Y232W mutants, inwhich the Tyr2 32 residues were replaced by alanine(Y232A) and tryptophan (Y232W), respectively, by sitedirected mutagenesis, and purified them ... (lanes 2, 6, 10, 14, 18, 22 , 26 , and30), 2 lM (lanes 3, 7, 11, 15, 19, 23 , 27 , and 31), and 4 lM (lanes 4, 8, 12, 16, 20 , 24 , 28 , and 32) .Y. Matsuo et al. Roles of the HsRad51 L1 and L2 loopsFEBS...