Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
... 313–326 ª 2004 FEBS Defining the Q P -site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy Richard A. Rothery 1 , Andrea M. Seime 1 , ... effects on the EPR properties of FrdABCD. In the case of the FrdB-T205H mutant, the [3Fe-4S] cluster line-shape is narrower than that of...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS docx
... contribution of the catabolic pathways to the formation of the serine pool from [1- 13 C]glucose experiments. (C) Relative contribution of the catabolic pathways and malic enzyme to the formation of the ... (1967) The labeling of pentose phosphate from glucose- 14 C and estimation of the rates of transaldolase, transketolase, the contribution of the pen...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... explain the dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural ... bc 1 sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of the l...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... subfamily together with the intermediary enzymes (Fig. 1). The overall arrangement of the tree is that the clusters of true rBAT and 4F2hc proteins are separated from each other by the GH13 enzymes. All ... discriminating the 4F2hc proteins from both rBATs and GH13 enzymes is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connect...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... illustrates the quality of the experimental data (Fig. 2B). Reconstruction of the overall shape of the PLDa2 from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The ... should occupy 16% of the molecular volume, and therefore seems too large to represent the lid-like structure at the top of the left view in Fig. 3. We...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... decreased the expression levels of HO-2 mRNA and protein in Jurkat T-lymphocytes and YN-1 and K562 erythroid cell lines. To further analyze the effects of hypoxia on the expression of HO-1 and HO-2, ... the expression levels of HO-1 and HO-2 proteins, detected by western blot analysis. Opposite effects of hypoxia on expression of HO-1 and HO-2 mRNA in YN-1 ce...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... unveil the sta- tus of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses the recombinase activity of OsDmc1, as has ... Schematic representation of the renaturation (A) and strand exchange (B) reactions mediated by OsDmc1 protein. Phi-C and Phi-W represent rhodamine and fluorescein carr...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... explained by elec- trostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue at position 149. On the other hand, as agmatine lacks the a-carboxyl group, there ... corrected by substracting the spectrum of the buffer solution (5 mm Tris ⁄ HCl, pH 7.5) in the absence of protein. The stability to thermal inactivation was...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... GavÞ 2 r ð4Þ where Ri and Gi are the red and green intensities of voxel I, respectively, and Rav and Gav the average values of Ri and Gi, respectively. It is used for describing the correlation of the intensity distributions ... D 2 R1, D 2 R2, D 2 R3). These experiments were performed to determine the influence of the introduced mutations on the localization o...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
... refers to the c.p.m. value of the test reaction minus the c.p.m. value from the control reaction (reaction terminated immediately after the addition of [ 14 C]arginine). The average c.p.m. for the ... dimension, chloroform ⁄ methanol ⁄ acetic acid (2 : 4 : 4). The plate was then autoradiographed. The markers were visualized by ninhydrin stain- ing, and then marked as...
Ngày tải lên: 07/03/2014, 05:20