Báo cáo khoa học: Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli pptx

... one of these domains and compare their activities and stabilities with those of the intact protein. In this report, the N- and C-domains of SIB1 FKBP22 were overproduced in E. coli and purified in an ... which are spanned by a 40 amino acid long a3 helix. The N-domain consists of a1 and a2 helices and an N-terminal region of a3 helix. The...

Ngày tải lên: 23/03/2014, 13:20

... Surolia N, RamachandraRao SR & Surolia A (2002) Paradigm shifts in malaria parasite biochemistry and anti-malarial chemotherapy. Bioessays 24, 192–196. 5 Ramya TNC, Surolia N & Surolia A ... occurrence of a different type (type I) of fatty acid biosynthetic pathway in the human host from that of the malaria parasite, make this pathway an attractive target for d...

Ngày tải lên: 16/03/2014, 10:20

... the rate of product accumulation (Table 1). These data demonstrate that ChlH enhances both the accumulation and decay of this reaction intermediate, resulting in a reduction in the lag phase of ... show that the magnesium chelatase H subunit markedly enhances ChlM catalysis by accelerating the formation and breakdown of the catalytic intermediate, providing a k...

Ngày tải lên: 20/02/2014, 02:21

... surface of the structural unit that contains the catalytic site, rather than on an auxiliary domain [9]. These substrate bind- ing sites are located at a certain distance from the active site and ... al. [34]. The protein conce- ntration was calculated based on the values obtained for alanine, threonine, glycine, valine, phenylalanine and aspar- tate. The analysis, start...

Ngày tải lên: 14/02/2014, 19:20

... of conveying the same in- formation in different ways are referred to by the term paraphrase and in the case of single words sharing the same meaning we speak of synonyms. Identification of synonyms ... mul- tilingual setting. In particular, translations of a word into other languages found in parallel cor- pora are seen as the (translational) context of tha...

Ngày tải lên: 20/02/2014, 12:20

... donors (in the parentheses). In all cases, the activity of one subfraction with maximal activity was taken as 100% and the activity of other subfractions was calculated as a percentage of that with ... mothers contains subfractions of 150-kDa IgG and 360-kDa sIgA antibodies, which hydrolyze DNA, RNA, and ATP [25,35]. After separation of human milk proteins by SDS/PA...

Ngày tải lên: 21/02/2014, 00:20

... CCTTTGAGCACATTTCGGCAA-3¢ P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 289 5¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢ b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 665 5¢-3222 AGCCATGCCAAATGTCTCAT-3¢ Fig. 1. Dose-related ... AGGTGCTTTGCCAGATATGACT-3¢ 432 5¢-802 CTCTTTGATGACAGAAGTGCCT-3¢ Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 365 5¢-450 AAAAATGTTGCTGCCCTG-3¢ Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢...

Ngày tải lên: 21/02/2014, 03:20

... statements, especially to novices. The ambiguity of natural lang- uage may also interfere with careful thinking about the data stored in the machine. An understanding of onto/into mappings, ... con- trasting natural language and proposed alternatives in paper and pensil studies. Subjects may be asked to write queries to a database of present a sequence of comm...

Ngày tải lên: 21/02/2014, 20:20

... 666 and 670, within one b-strand of domain V, are also important for interdomain interac- tions. The N-terminal part of the strand interacts with the N-terminal part of helix C G in domain G, and ... superimposed on the basis of domains I and II. Looking from the direction of the arrow in (A) and (B), the coordinates of His572 at the tip of domain I...

Ngày tải lên: 06/03/2014, 22:21

... forward, CAGGAAGAACCGTTGGAGTT; reverse, TTGCTCTCGTTCCAAAAGGA Actb forward, AAGGAAGGCTGGAAAAGAGC reverse, TACAGCTTCACCACCACAGC Hmgcs1 forward, TTTGATGCAGGTGTTTGAGG reverse, CCACCTGTAGGTCTGGCA Sqstm1 ... AGTCCATGAGTTGGCCCATA Egr1 forward, CCTATGAGCACCTGACCACA reverse, AGGCCACTGACTAGGCTGAA Egr1 pre-mRNA forward, GAGCAGGTCCAGGAACATTG reverse, GGGATAACTCGTCTCCACCA Ndrg1 forward, ACCTGCTACAACCCCCTC...

Ngày tải lên: 07/03/2014, 03:20