... similarity with the other members of the IL-1family; 30–38% with IL-1a, 26–36% with IL-1b and 30% with IL-1Ra (data not shown). To further analyse the relationship of trout IL-18 with the other ... extracted twice with an equal volume of phenol/chloroform (24 : 1, v/v) and once with chloroform. Genomic DNA was precipitated with two volumes of cold ethanol, washed with 70% (v/v)ethanol, and dissolved ... tetraodon, rather than the IL-1bgenes, in terms of exon/intron organization, the size of coding exons and the precursor molecules (Fig. 2). The relationship between the trout IL-18 with the IL-1...