Báo cáo khoa học: Monitoring ligand-mediated nuclear recepto

Báo cáo khoa học: Monitoring ligand-mediated nuclear receptor–coregulator interactions by noncovalent mass spectrometry pptx

... lexes by ion s pray mass spectrometry. J. Am. Chem. Soc. 116, 7487–7493. Ó FEBS 2004 ESI-MS to investigate noncovalent complexes (Eur. J. Biochem. 271) 4967 Monitoring ligand-mediated nuclear receptor–coregulator ... myoglobin by electrospray-ionization mass spectrometry. J. Am. Chem. Soc. 113, 8534–8535. 50. Chowdhury, S.K., Katta, V. & Chait, B.T. (1990) Probing con...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Control of nuclear receptor function by local chromatin structure doc

... followed by a chromatin transition and possibly by changes in at least some of the chromatin signatures [80,81]. Dynamic changes of histone modiﬁcations after hormone induction have not been Nuclear ... by promoting chromatin looping at the Dlx5 gene with the loop itself marked by an H3-K9me2 repressive mark [184]. A very recent report describes MeCP2 in neuronal cells as a highly...

Ngày tải lên : 28/03/2014, 22:21

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Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx

... metal enzyme interactions. J. Am. Chem. Soc. 74, 1242–1247. Ó FEBS 2003 Kinetics of nick sealing by T4 DNA ligase (Eur. J. Biochem. 270) 4325 Kinetics and thermodynamics of nick sealing by T4 DNA ... 1min )1 ). Therefore, binding of ATP could by no means limit the enzyme turnover, unless inhibition by DNA is considered. Furthermore, the apparent K d for ATP in the noncovalent EÁATP...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: Peroxisome proliferator-activated receptor a plays a vital role in inducing a detoxiﬁcation system against plant compounds with crosstalk with other xenobiotic nuclear receptors docx

... mouse liver and intestine by a PPARa agonist Wy-14 643 and by sesame seeds. A,B: Immunoblot analysis of 17b-HSD11 induction in the mouse liver and intestine by Wy-14 643 or by various plant seeds ... intestinal proteins were separated by SDS ⁄ PAGE and the sesame-induced 24 kDa protein band (A) was analyzed by peptide mass ﬁngerprinting after digestion by lysyl-endopeptidase....

Ngày tải lên : 16/03/2014, 14:20

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Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... processing. This shedding is mediated by ADAM (a disintegrin and metalloprotease) proteins that are membrane-anchored metalloproteases [4]. Signal transduction by ERBB proteins Binding of a family ... STAT pathway activation [19]. Whereas transformation by most EGFR mutants con- fers cell sensitivity to erlotinib and geﬁtinib, transformation by an exon 20 insertion (D770insNPG) make...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... tyrosine kinase domain of the receptor and thereby stabilize the interaction with EGF-TKIs, leading to an increase of  100-fold in sensitivity to inhibition by EGFR-TKIs compared with that of the wild-type receptor ... 2009, accepted 8 October 2009) doi:10.1111/j.1742-4658.2009.07449.x The discovery that signaling by the epidermal growth factor receptor (EGFR) plays a key role in tumorig...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... the nuclear extract of PMA-differentiated THP-1 macrophages stimulated by HDL 3 . The speciﬁcity of the assay was veriﬁed by using mutant oligonucleotides, which failed to bind to KLF4, and by ... determined by real-time PCR. (B) Protein levels of KLF4 were determined by western blot. (C) mRNA levels of hSR-BI were determined by real-time PCR. (D) Protein levels of hSR-BI were d...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high speciﬁc activity from Pisum sativum catalytically translocates in the 3¢ﬁ5¢ direction docx

... and Mg 2+ (data not shown). Native molecular mass of PDH120 The native molecular mass of PDH120 was determined by its hydrodynamic properties, i.e. by glycerol gradient centrifugation (Fig. 2A) ... and the reaction was terminated by addition of 1.5 lL75m M EDTA, 2.25% SDS, 37.5% (by vol.) glycerol and 0.3% Bromophenol blue. The reaction products were separated by 12% native PAGE...

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

... regulated by EGF treat- ment and reversed by gefitinib). Among these candi- dates, miR-125a-5p appeared to be particularly intriguing, because its level was altered most signifi- cantly by EGF stimulation ... let-7i, miR-24, miR-25, miR- 29b, and miR-125a-5p, could be reversed by gefitinib (Fig. 1B). We further verified our array results by quantitative PCR, which revealed the expressio...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc

... supported by gra from the Australian National Health and Medical Research Council (NHMRC) and the Australian Research Council. D.T. is supported by an NHMRC Principal Research Fellowship, and M.M. by ... ﬂuo- ride, and centrifuged at 800 g at 4 °C for 10 min to obtain a postnuclear supernatant. Mitochondria were pelleted by centrifugation at 10 000 g at 4 °C for 20 min. In vitro impor...

Ngày tải lên : 07/03/2014, 00:20

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