Báo cáo khoa học: Monitoring ligand-mediated nuclear receptor–coregulator interactions by noncovalent mass spectrometry pptx

... lexes by ion s pray mass spectrometry. J. Am. Chem. Soc. 116, 7487–7493. Ó FEBS 2004 ESI-MS to investigate noncovalent complexes (Eur. J. Biochem. 271) 4967 Monitoring ligand-mediated nuclear receptor–coregulator ... myoglobin by electrospray-ionization mass spectrometry. J. Am. Chem. Soc. 113, 8534–8535. 50. Chowdhury, S.K., Katta, V. & Chait, B.T. (1990) Probing con...

Ngày tải lên: 23/03/2014, 13:20

... followed by a chromatin transition and possibly by changes in at least some of the chro- matin signatures [80,81]. Dynamic changes of histone modifications after hormone induction have not been Nuclear ... by promoting chromatin looping at the Dlx5 gene with the loop itself marked by an H3-K9me2 repressive mark [184]. A very recent report describes MeCP2 in neuronal cells as a highly...

Ngày tải lên: 28/03/2014, 22:21

... metal enzyme interactions. J. Am. Chem. Soc. 74, 1242–1247. Ó FEBS 2003 Kinetics of nick sealing by T4 DNA ligase (Eur. J. Biochem. 270) 4325 Kinetics and thermodynamics of nick sealing by T4 DNA ... 1min )1 ). Therefore, binding of ATP could by no means limit the enzyme turnover, unless inhibition by DNA is considered. Furthermore, the apparent K d for ATP in the noncovalent EÁATP...

Ngày tải lên: 21/02/2014, 00:20

... mouse liver and intestine by a PPARa agonist Wy-14 643 and by sesame seeds. A,B: Immunoblot analysis of 17b-HSD11 induction in the mouse liver and intestine by Wy-14 643 or by various plant seeds ... intestinal proteins were separated by SDS ⁄ PAGE and the sesame-induced 24 kDa protein band (A) was analyzed by peptide mass fingerprinting after digestion by lysyl-endopeptidase....

Ngày tải lên: 16/03/2014, 14:20

... processing. This shedding is mediated by ADAM (a disintegrin and metalloprotease) proteins that are membrane-anchored metalloproteases [4]. Signal transduction by ERBB proteins Binding of a family ... STAT pathway activation [19]. Whereas transformation by most EGFR mutants con- fers cell sensitivity to erlotinib and gefitinib, transfor- mation by an exon 20 insertion (D770insNPG) make...

Ngày tải lên: 16/02/2014, 09:20

... tyrosine kinase domain of the receptor and thereby stabilize the interaction with EGF-TKIs, leading to an increase of  100-fold in sensitivity to inhibition by EGFR-TKIs compared with that of the wild-type receptor ... 2009, accepted 8 October 2009) doi:10.1111/j.1742-4658.2009.07449.x The discovery that signaling by the epidermal growth factor receptor (EGFR) plays a key role in tumorig...

Ngày tải lên: 16/02/2014, 09:20

... the nuclear extract of PMA-differentiated THP-1 macrophages stimulated by HDL 3 . The specificity of the assay was verified by using mutant oligonucleotides, which failed to bind to KLF4, and by ... determined by real-time PCR. (B) Protein levels of KLF4 were determined by western blot. (C) mRNA levels of hSR-BI were determined by real-time PCR. (D) Protein levels of hSR-BI were d...

Ngày tải lên: 18/02/2014, 04:20

... and Mg 2+ (data not shown). Native molecular mass of PDH120 The native molecular mass of PDH120 was determined by its hydrodynamic properties, i.e. by glycerol gradient centrifugation (Fig. 2A) ... and the reaction was terminated by addition of 1.5 lL75m M EDTA, 2.25% SDS, 37.5% (by vol.) glycerol and 0.3% Bromophenol blue. The reaction products were separated by 12% native PAGE...

Ngày tải lên: 20/02/2014, 11:20

... regulated by EGF treat- ment and reversed by gefitinib). Among these candi- dates, miR-125a-5p appeared to be particularly intriguing, because its level was altered most signifi- cantly by EGF stimulation ... let-7i, miR-24, miR-25, miR- 29b, and miR-125a-5p, could be reversed by gefitinib (Fig. 1B). We further verified our array results by quantitative PCR, which revealed the expressio...

Ngày tải lên: 07/03/2014, 00:20

... supported by gra from the Australian National Health and Medical Research Council (NHMRC) and the Australian Research Council. D.T. is supported by an NHMRC Principal Research Fellowship, and M.M. by ... fluo- ride, and centrifuged at 800 g at 4 °C for 10 min to obtain a postnuclear supernatant. Mitochondria were pelleted by centrifugation at 10 000 g at 4 °C for 20 min. In vitro impor...

Ngày tải lên: 07/03/2014, 00:20