Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... NMR analysis (Eur. J. Biochem. 271) 4949 Total chemical synthesis and NMR characterization of the glycopeptide tx 5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure ... glycosylated at position 3. Taken together, these data identified the glycan as a- D -Gal-(1fi3)- a- D -GalNAc. There a re several NOEs bet...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... in A. thaliana PS. Val111 and Gly113, on the other hand, are both affected by both pantoate and ATP. These residues have their positional equivalents in Val137 and Arg139 in A. thaliana PS, as ... coli and M. tuberculosis. Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A....
Ngày tải lên: 16/02/2014, 09:20
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are ... cotransport of a Fig. 4. Subs trate apparent K m values as a function of the extracellular pH. (A, B) Membrane potential dependency of the apparent K m values of gly...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt
... phospho- ferrochelatase of S13 0A and S30 3A was decreased, and the band was not detected in the double mutant S13 0A and S30 3A, indicating that ferrochelatase was phosphorylated at positions 130 and 303. The reaction of ... human ferrochelatase S13 0A, S30 3A and S33 0A were prepared: in the first round of PCR, human ferrochelatase [43] was used as a template...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase doc
... 1). Based on the above comparisons, it appears that all three FlgCK domains have the requisite elements for catalysis and are at least capable of the same types of structural interactions described ... domains of FlgCK, and lays the groundwork for study of the relationship between these functional properties and the structural interac- tions that potentially medi...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications pot
... broad-spectrum anticancer and antibacterial activity of membrane active peptides, suggests that the anticancer activity is also due to penetration and disruption of the membranes of the cancer cells. ... (SA) is the mean structure obtained by best-fitting and averaging the coordinates of backbone N, a- C and carbonyl-C atoms of the 20 final structures. (SA...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
... 5¢-GC GGAATTCAAAAAGAGAAAGAAAGGG-3¢ and MALC: 5¢-GGCCTGCAGCTAGAGAAGTGAAACATC-3¢ with HAKN1 cDNA [28] as the template. An I50S mutant was constructed using complementary oligonucleotides I50SF and I50SR ... homeodomain–DNA interactions FEBS Journal 272 (2005) 190–202 ª 2004 FEBS 199 MAT1 (5¢-AGGGGACATAGATTTACAAAAGAAGCTC GTCAACAA-3¢; first round, MATa2 sequences underlined) or MAT2 (5¢-CGCG...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx
... functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 Naoko Uekama, Takio Sugita, Masashi Okada, Hitoshi Yagisawa and Satoru Tuzi Graduate ... diffraction. As shown with dotted lines, the chemical shifts of the sig- nals of Ala116 and Ala118 are not affected by addition of PS, indicating that conformations...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx
... metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein Laura Villarreal 1 , Laura Tı ´o 2 , Merce ` Capdevila 1 and Sı ´lvia Atrian 2 1 Departament de Quı ´ mica, ... 157–166. 18 Capdevila M, Cols N, Romero-Isart N, Gonzalez-Duarte R, Atrian S & Gonzalez-Duarte P (1997) Recombinant synthesis of mouse Zn 3 -b and Zn 4 -a metallothionein 1...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
... nuclear factor Y )1203 (+) 1.000 0.937 aggacCCAAtaaggga V$GATA/GATA2.02 GATA-binding factor 2 )177 (–) 1.000 0.912 agcGATAtta V$GATA/GATA1.03 GATA-binding factor 1 )672 (–) 1.000 0.954 taaaGATAaacaa V$GATA/GATA1.02 ... were synthesized based on the linker sequence (C1: 5¢-GTAC ATATTGTCGTTAGAACGCGTAATACGACTCA-3¢; C2: 5¢-CGTTAGAACGCGTAATACGACTCACTATA GGGAGA-3¢). First round PCR was performed...
Ngày tải lên: 31/03/2014, 07:20