Báo cáo khoa học: Trans-splicing of a mutated glycosylasparaginase mRNA sequence by a group I ribozyme deficient in hydrolysis pptx
Báo cáo khoa học: Trans-splicing of a mutated glycosylasparaginase mRNA sequence by a group I ribozyme deficient in hydrolysis pptx
... Additional bands result from degradation of RNA during incubation at trans-splicing and RPA hybridization
conditions. Below; quantitation of RPA of trans-spliced GA mRNA generatedbyDiGIR2AGUandDiGIR2DP9.2 ... hydrolysis.
Keywords: glycosylasparaginase mRNA; group I i ntron;
ribozyme hydrolysis; RNA reprogramming; trans-splicing.
Group I ribozyme- mediated RNA r epr...
Báo cáo khoa học: Pharmacology of vascular endothelium Delivered on 27 June 2004 at the 29th FEBS Congress in Warsaw pptx
... general
rule: in vitro cognitio sed in vivo veritas.
Abbreviations
AA, arachidonic acid; ACE -I, angiotensin converting enzyme (and kininase 2) inhibitors; ADMA, asymmetric dimethylarginine; ASA,
acetylsalicylic ... Only ADMA is biologically active, i. e. it acts
as endogenous inhibitor of eNOS and inhibitor of Arg
membrane transporter. Clinical data on ADMA are
growing. A high pla...
Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx
... these data indicate that an
intact active site of MVK is required for its binding to rat LHR mRNA
and for its translational suppressor function.
Abbreviations
GHMP, galactokinase homoserine kinase ... function protein, pos-
sessing both catalytic function and LHR mRNA bind-
ing activity. This RNA binding activity of MVK leads
to translational suppression and triggers LHR mRNA
degrad...
Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
... overexpres-
sed in yeast and purified by Ni
2+
–nitrilotriacetic acid
agarose (NiNTA), is recognized by the antibodies,
indicating that the protein is phosphorylated in vivo at
Ser258. The antibodies are ... Raffaele Lopreiato
1,
*, Elena Casanova
1
, Maria Ruzzene
1,2
, Sonia Facchin
1
,
Lorenzo A. Pinna
1,2
, Giovanna Carignani
1
and Geppo Sartori
1
1 Dipartimento di Chimica Biologi...
Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... transfection assays with increasing amounts
of DNA fragments encoding cyt b
5
and monitored the
formation of androstadienol and DHEA. As shown in
Fig. 5 the stimulation of DHEA and androstadienol
production ... cross-talk interactions with other nuclear recep-
tors and with a broad range of other intracellular signaling
pathways, including those activated by certain cytokines
an...
Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot
... the
phosphorylation status of p65 with its C-terminal transac-
tivation domain, either directly or indirectly. It is possible
that PKA inhibits NF-jB in a multiplicative way including
stabilization of inhibitor ... suggested
that PKA inhibited NF-jB activation by stabilizing IjB,
PKA activating agents such as FSK did not significantly
affect the TNFa-induced IjBa degradation or DNA-...
Báo cáo khoa học: "EVALUATION OF NATURAL LANGUAGE INTERFACES TO DATABASE SYSTEMS: A PANEL DISCUSSION " ppt
... EVALUATION OF NATURAL LANGUAGE INTERFACES TO DATABASE SYSTEMS:
A PANEL DISCUSSION
Norman K. Sondheimer, Chair
Sperry Univac
Blue Bell, PA
For a natural language access to database
system ... be practical it must achieve a good
match between the capabilities of the user
and the requirements of the task. The user
brings his own natural language and his own
style of intera...
Báo cáo khoa học: Binding of non-natural 3¢-nucleotides to ribonuclease A ppt
... arabinonucleotides are effective
antisense agents [40]. In addition, the antineoplastic
drug fludarabine is an arabinonucleoside, and the
antineoplastic drug clofarabine is a 2¢-fluoro-2¢-deoxy-
arabinonucleoside. ... A and 3¢-nucleotides, and indicate that non-natural fura-
nose rings can serve as the basis for more potent inhibitors of catalysis by
RNase A.
Abbreviations
araUMP, a...
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... an adequate antiviral immune response.
After stimulation of distinct TLR pathways different
MAP kinases and several transcription factors like
Nf-jB are activated and the induction of proinflamma-
tory ... MAP kinases. It is possible
that the modulation is due to the activation of JNK.
Other MAP kinase inhibitors as well as G-protein
inhibitors should be investigated to find out the parti...
Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... 4C is a clear indication that the mechanism of
regulation of dCTP deaminase is not a simple rapid
equilibrium mechanism. The observed increase in
cooperativity of dTTP inhibition at increasing ... structural and mechanistic studies on dTTP
inhibition of E. coli dCTP deaminase. Coordinated
closure of the active site and rearrangement of the
main chain and side chains in the...