Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt

... Aptamer probes of RNA polymerase structure (Eur. J. Biochem. 271) 4931 Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB Andrey Kulbachinskiy 1,2 , ... when GreB w as added b efore the aptamers (Fig. 5 B a nd data not shown). Specific and nonspecific interactions of aptamers with the RNAP main ch...

Ngày tải lên: 23/03/2014, 13:20

... how the interaction between 14-3-3 and ExoS occurs, our pull-down analysis with GST-ExoS(LDL426– 428AAA) still strongly suggests that ExoS must utilize a strategy for its interaction with 14-3-3 that ... mediates the interaction of 14-3-3 with the phospho-amino acid residues in its interaction partners. Therefore it is likely that each dimer contains two binding pock...

Ngày tải lên: 19/02/2014, 08:20

... ion channel [1,2]. The a and b subunits of the Torpedo receptor referred to in this report correspond to the a1 and b1 subunits in the nomenclature recommended by the International Union of Pharmacology ... sites for agonists and competitive antagonists [4,5]. It is now generally agreed that these sites lie at the interfaces between the a–c and the a–d subunits [6]. Labeling and m...

Ngày tải lên: 19/02/2014, 07:20

... nicotinic and muscarinic ligands, suggesting that WTX interacts with both nAChR and mAChR. Experiments using hetero- logous expression of mAChRs revealed that the mech- anism of WTX interaction with ... membranes by muscarinic ligands demon- strates a specific interaction of WTX with mAChRs. WTX interaction with heterologously expressed mAChRs To characterize the WTX int...

Ngày tải lên: 23/03/2014, 05:22

... humans and yeast [6–8] and its loss leads to genome i nstability and chromosomal end fusion through the activation of DNA damage checkpoints [5,9]. Proteins associated with both double-stranded and G-rich ... 3051 sense and antisense oligonucleotides, as described by Cano et al . [28]. Fully partial duplex and double-stranded DNA (dsDNA) were purified from the residual single...

Ngày tải lên: 23/03/2014, 13:20

... part of the word. Its performance was compared with a stemmer using hand crafted stemming rules, the Euroling SiteSeeker stemmer for Swedish, Danish and Norwegian, and also with a stemmer for ... finite time. To keep the number of candidate rules within practical limits, we used the strategy that the pat- tern of a candidate is minimally different from its parent’s patter...

Ngày tải lên: 23/03/2014, 16:21

... stand on his head, and intends his doing the latter to play a role in his plan to do the former and that he also believes that he can talk to Kathy, believes that he can find out how to stand ... just that. And if R further believes that the system manager can override file permissions and that Tom is the system man- ager, but also that Q does not know the former f...

Ngày tải lên: 31/03/2014, 17:20

... of RNA 9A; lanes 7, 8, and 9: 20, 100, and 200 pmol of RNA 9B. (B)Lanes 1and2 ,noRNA;lanes3,4,5,6,7 ,and8 ,10,20,40,60,80, and 100 pmol of RNA 9 A (C) Lanes 1 and 2, no RNA; l anes 3, 4, 5, 6, 7, and ... we isolated and characterized RNA aptamers to the Raf-1 RBD, and inhibited the Ras-induced Raf-1 activation by these aptamers. The aptamers, RNAs 9A and 9 B, prev...

Ngày tải lên: 23/03/2014, 21:21

... (EMSA) with EWS and several G-quadruplex or single- or dou- ble-stranded DNA and RNA. Here, we show that EWS specifically targets G-quadruplex DNA and RNA in vitro. We also determined that the ... performed with EWS (lanes 2 and 4) and 32 P-labeled ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per- formed with EWS (lanes 2, 4 and 6) and 32 P-labe...

Ngày tải lên: 15/02/2014, 01:20

... blue and grey) in complex with its physiological RNA target (blu ⁄ green and grey). Recognition of the RNA A-form helix is mediated by contacts between by loops 2 and 4 and the RNA minor and major ... in complex with an RNA hairpin [31] and the one of Escherichia coli RNaseIII dsRBM in complex with dsRNA [35], confirmed the existence of a common interaction pat- t...

Ngày tải lên: 19/02/2014, 17:20