Báo cáo khoa học: Structural basis for stereo-specific catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

... substrate specificity for its native substrate l-arginine, but also tolerates l-homoarginine and l-canavanine (Fig. 3A, Table 1). These findings can be explained by the coor- dination of l-arginine in the active ... methylene group in the side chain of l-arginine, as exemplified by the binding and catalytic turnover of l-homoarginine. Concerning l-canavanine turnover by VioC, the modified g...

Ngày tải lên: 16/03/2014, 02:20

... maltodextrin- binding protein (TvuCMBP) complexed with c-cyclodextrin has been deter- mined. Like Escherichia coli maltodextrin-binding protein (EcoMBP) and other bacterial sugar-binding proteins, TvuCMBP ... [8,9]. The malto- dextrin-binding protein from E. coli (EcoMBP) is also widely used as a tool for genetic engineering [10] and as a biosensing platform [11,12]. The ATP-binding cas-...

Ngày tải lên: 19/02/2014, 00:20

... spectrum. Therefore, in order to gain a glo- bal insight into metal ion–RNA interactions, it is often necessary to conduct structural studies of metal ion binding modes in RNA molecules using biophysical methods ... and G38 in aptamer no. 18, as well as G25 and G26 or G39 and G40 in aptamer no. 20, in Co 2+ binding. Interestingly, a similar metal ion binding mode to the tandem gua...

Ngày tải lên: 16/03/2014, 06:20

... [42]. Molecular modeling Docking experiments and binding energy calculations were performed with leadfinder [23]. This program was also used for the preparation of the protein structure for dock- ing by addition ... claim to original Slovakian government works 2107 interesting that the side chain conformations of amino acids interacting with MeGlcA 2 Xyl 3 did not change as a result of bi...

Ngày tải lên: 22/03/2014, 16:20

... are summarized in Table 3. As is usually observed for protein:carbohy- drate interactions, the binding constants are in the mil- limolar range. Binding of mannose in the primary binding site is enthalpy-driven. ... obtain the binding constant K b and binding enthalpy DH b from the measured heat release Q i on each injection using the Wiseman isotherm [46]. Free energy and entropy of...

Ngày tải lên: 23/03/2014, 11:20

... Martins et al. NAD + -dependent (R) -2-hydroxyglutarate dehydrogenase FEBS Journal 272 (2005) 269–281 ª 2004 FEBS 281 Structural basis for stereo-specific catalysis in NAD + -dependent (R )-2-hydroxyglutarate ... nucleotide- binding domain displays a well-preserved fold indicating its evolutionary structural conservation (as expected from its role in coenzyme bin...

Ngày tải lên: 23/03/2014, 13:20

... not impair the interaction, indi- cating that the glutamine residue is not absolutely required for binding and is most likely involved in increasing binding affinity. G426 of GRINL1A occu- pies ... staining and immunogold labelling [26]. A non-consensus sequence within GRINL1A is responsible for DYNLL1 binding in the canonical mode In this study, we have identified GRINL1A as a novel...

Ngày tải lên: 29/03/2014, 09:20

... were transformed into KY895 and then the strains were examined for growth, +, in the presence of different concentrations of thymidine in the medium. In the last column, LD 100 values are given (in ... deoxyribonucleosides; dT, deoxythymidine; dU, deoxyuridine; dC, deoxycytidine; dA, deoxyadenosine; dG, deoxyguanosine; hTK1, human thymidine kinase 1; HSV1-TK, Herpes simplex virus 1 t...

Ngày tải lên: 30/03/2014, 20:20

... kcalÆmol )1 ÆA ˚ )2 for all nOes involving nonexchangeable protons, 10 kcalÆmol )1 ÆA ˚ )2 for all nOes involving exchangeable protons and the atoms involved in H-bonds. For the dihedral angle restraints a force ... reference. Starting structure and structural restraints The starting structures for both U1- and U3-hairpins were generated u sing the molecular mod elling p ackage IN...

Ngày tải lên: 31/03/2014, 09:20

... conserved N-terminal domain containing leucine-rich repeats (LRRs), motifs known to mediate protein–protein inter- actions, and of a C-terminal low-complexity region, mainly composed of polyglutamates. Since ... N-termi- nus (1–164 amino acids) was cloned into the pGBKT7 vec- tor (Clontech, Mountain View, CA, USA) for expression as a Gal4 DNA-binding domain fusion protein. This bait was tra...

Ngày tải lên: 18/02/2014, 17:20