Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

... Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase Bjarne Hove-Jensen 1 and James N. McGuire 2 1 Department ... the structu re of B. subtilis 5-phospho-a- D -ribosyl 1 -diphosphate synthase shows 92% of the amino acid differences to be on solvent exposed surfaces in the hexameric structure. Keywords:...

Ngày tải lên: 23/03/2014, 13:20

... Linguistics Correcting Automatic Translations through Collaborations between MT and Monolingual Target-Language Users Joshua S. Albrecht and Rebecca Hwa and G. Elisabeta Marai Department of Computer Science University ... This paper describes a collabora- tive approach for mediating between an MT system and users who do not under- stand the source language and thus cannot easily...

Ngày tải lên: 31/03/2014, 20:20

... the purified (lanes 1, 3 and 5) and recombinant (lanes 2, 4 and 6) enzymes were used. Lanes 1 and 2, E. quadrivirgata enzyme; lanes 3 and 4, E. climacophora enzyme; lanes 5 and 6, A. blomhoffii enzyme. ... discussed. Materials and methods Materials and biological samples Three different species of snake, E. quadrivirgata, E. climacophora and A. blomhoffii weighing about 210 g (11...

Ngày tải lên: 20/02/2014, 23:20

... L 1 and L 2 . All these amino acids have a surface area above 180 A ˚ and a hydrophobicity index close to 0 or above (Table 2). The inability to turn over amino acids with more hydrophobicity and ... L 2 between: (D) OAB as indicated and 0 lM ( ), 5 lM (·), 10 lM ( ) and 20 lM ( ) OAB; (E) NATA as indicated and 0 l M ( ), 5 lM ( ), 10 lM ( ) and 20 lM (·) OAB; and (F) N...

Ngày tải lên: 07/03/2014, 05:20

... N-termi- nal amino acid sequence of LSFRAHLSD was encoded by nucleotides 183–209. The internal amino acid sequences RTFEVNAHPDIL, SADQLLQQAL and SEGRLHFAGEHT were found at positions 567–602, 636–675 and ... identified this antibacterial protein as a novel l -amino acid oxidase (LAAO; EC.1.4.3.2). LAAOs catalyze the oxidative deamination of an l -amino acid substrate and hav...

Ngày tải lên: 29/03/2014, 08:20

... proliferation and growth, cytokinesis, replication, embryogenesis and nucleogenesis [22]. More recently, nucleolin has been described as a shuttle between the cell surface and the nucleus [17,21,23] and ... is a major Lf-binding site at the surface of cells. As nucleolin is a ubiquitous multiligand protein known for its shuttle function between the cell surface and the nuc...

Ngày tải lên: 07/03/2014, 15:20

... c-kitC6, c-kitC8 and c-kitC12 sequences at pH 6.0 and 7.0. Fig. S4. Normalized UV annealing and melting curves for c-kitC0, c-kitC4, c-kitC6, c-kitC8 and c-kitC12 complementary strands at pH 6.0 and 7.0. Fig. ... C-rich strands, we performed CD stud- ies (Fig. S3) and UV melting studies at 287 and 265 nm (Fig. S4) at both pH 6.0 and 7.0. Both CD and UV melting studies showed...

Ngày tải lên: 07/03/2014, 02:20

... recognition and interaction events between the LHCP substrate and the cpSRP subunits. Binding between LHCP and cpSRP43 is med- iated by a conserved 18 amino acid span, termed L18, positioned between ... of the eukaryotic SRP receptor, SRa, and the prokaryotic FtsY [61]. The exact parti- tioning of cpFtsY between the stroma and thylakoid membrane is unclear and may be t...

Ngày tải lên: 07/03/2014, 03:20

... archaeal geranylgeranyl diphosphate synthase to farnesyl diphosphate synthase. Two amino acids before the first aspartate-rich motif solely deter- mine eukaryotic farnesyl diphosphate synthase activity. J ... geranyl- geranyl diphosphate synthase from the bacterium, Pantoea ananatis, was studied. In most types of short-chain (all-E) prenyl diphosphate synthases, bulky amino...

Ngày tải lên: 07/03/2014, 06:20

... mm succinate and different amounts of CdCl 2 and CuCl 2 (0, 5 and 10 nmolÆmg protein )1 ). After 3 min of incubation, medium with mitochondria was collected, centrifuged at 10 000 g for 1 min at 4 °C and ... & Moore AL (1994) The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interplay between quinol-oxidizing and quinone-r...

Ngày tải lên: 16/03/2014, 02:20