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Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

... Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase Bjarne Hove-Jensen1 and James N. McGuire21Department ... the structu re ofB. subtilis 5-phospho-a-D-ribosyl 1 -diphosphate synthase shows 92% of the amino acid differences to be on solvent exposed surfaces in the hexameric structure.Keywords: kinetics; ... Consistent with the surface lo cation of thealtered amino acids were hydrophobicity surface maps ofmonomers from the two Bacillus PRibPP synthases.These revealed a n increase in polar surface area...
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Báo cáo khoa học:

Báo cáo khoa học: "Correcting Automatic Translations through Collaborations between MT and Monolingual Target-Language Users" pptx

... LinguisticsCorrecting Automatic Translations through Collaborations between MT and Monolingual Target-Language UsersJoshua S. Albrecht and Rebecca Hwa and G. Elisabeta MaraiDepartment of Computer ScienceUniversity ... This paper describes a collabora-tive approach for mediating between anMT system and users who do not under-stand the source language and thus cannoteasily detect translation mistakes on theirown. ... gainenough understanding of the source text to correctMT errors. The interactions between the users and the MT system may, in turn, offer researchers in-sights into the translation process and inspirationsfor...
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

... the purified (lanes 1, 3 and 5) and recombinant (lanes 2, 4 and 6) enzymes were used. Lanes 1 and 2, E. quadrivirgata enzyme; lanes 3 and 4, E. climacophoraenzyme; lanes 5 and 6, A. blomhoffii enzyme. ... discussed.Materials and methodsMaterials and biological samplesThree different species of snake, E. quadrivirgata,E. climacophora and A. blomhoffii weighing about 210 g(110 cm long), 270 g (130 cm long) and ... Elaphe quadrivirgata, Elaphe climaco-phora and Agkistrodon blomhoffii, and cloned their cDNAs.Each mature snake DNase I protein comprised 262 amino acids. Wild-type snake DNases I with Leu130...
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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf

... L1 and L2. All these amino acids have a surface area above 180 A˚ and a hydrophobicity index close to0 or above (Table 2). The inability to turn over amino acids with more hydrophobicity and ... L2 between: (D) OAB as indicated and 0 lM ( ), 5 lM (·), 10 lM ( ) and 20 lM ( )OAB; (E) NATA as indicated and 0 lM ( ), 5 lM ( ), 10 lM ( ) and 20 lM (·) OAB; and (F) NAT as indicated and ... hydrophobic amino acids with sur-face area smaller than 185 A˚2acted as poor substrates, and catalytic efficiencies with amino acids of surface area £ 155 A˚2were not detectable. On the other hand,l-Trp,...
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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx

Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx

... N-termi-nal amino acid sequence of LSFRAHLSD was encodedby nucleotides 183–209. The internal amino acid sequences RTFEVNAHPDIL, SADQLLQQAL and SEGRLHFAGEHT were found at positions 567–602,636–675 and ... identifiedthis antibacterial protein as a novel l -amino acid oxidase (LAAO; EC.1.4.3.2). LAAOs catalyze theoxidative deamination of an l -amino acid substrate and have been reported to exert antibacterial ... accession numberAB495360) and the derived amino acid sequence are shown. The N-terminal and internal peptide sequences of antibacterialprotein detected by amino acid sequencinganalysis are indicated...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx

Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx

... proliferation and growth,cytokinesis, replication, embryogenesis and nucleogenesis[22]. More recently, nucleolin has been described as a shuttle between the cell surface and the nucleus [17,21,23] and ... is a major Lf-binding site at the surface ofcells. As nucleolin is a ubiquitous multiligand proteinknown for its shuttle function between the cell surface and the nucleus, it may be expected ... (hLf).Sequences of the N2 and C2 domains of hLf, bovine lactoferrin (bLf) and human transferrin (hTf) were aligned and compared for similar-ities and divergences using theCLUSTALW and EDTALNprograms...
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Báo cáo khoa học: Effect of flanking bases on quadruplex stability and Watson–Crick duplex competition pptx

Báo cáo khoa học: Effect of flanking bases on quadruplex stability and Watson–Crick duplex competition pptx

... c-kitC6,c-kitC8 and c-kitC12 sequences at pH 6.0 and 7.0.Fig. S4. Normalized UV annealing and melting curvesfor c-kitC0, c-kitC4, c-kitC6, c-kitC8 and c-kitC12complementary strands at pH 6.0 and 7.0.Fig. ... C-rich strands, we performed CD stud-ies (Fig. S3) and UV melting studies at 287 and 265 nm(Fig. S4) at both pH 6.0 and 7.0. Both CD and UVmelting studies showed that the C-rich strands c-kitC0,c-kitC4 ... structuralcharacterization of nucleic acids in solution. TDS pro-vide a simple, inexpensive and rapid method to obtainstructural insight into nucleic acid structures, and maybe used for both DNA and RNA from short...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx

Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx

... recognition and interactionevents between the LHCP substrate and the cpSRPsubunits. Binding between LHCP and cpSRP43 is med-iated by a conserved 18 amino acid span, termed L18,positioned between ... of the eukaryotic SRP receptor,SRa, and the prokaryotic FtsY [61]. The exact parti-tioning of cpFtsY between the stroma and thylakoidmembrane is unclear and may be transient in nature,which ... complex (OE33) and plastocyaninabsolutely requires ATP [2–4]; light-harvesting chloro-phyll a ⁄ b-binding protein (LHCP) integration requiresGTP and is stimulated by ATP [5,6]; and transport...
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Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

... archaeal geranylgeranyl diphosphate synthase to farnesyl diphosphate synthase. Two amino acids before the first aspartate-rich motif solely deter-mine eukaryotic farnesyl diphosphate synthase activity.J ... geranyl-geranyl diphosphate synthase from the bacterium, Pantoea ananatis, wasstudied. In most types of short-chain (all-E) prenyl diphosphate synthases,bulky amino acids at the fourth and/ or fifth ... geranylgeranyl diphosphate synthase. AbbreviationsDMAPP, dimethylallyl diphosphate; FARM, first aspartate-rich motif; FPP, farnesyl diphosphate; FPS, farnesyl diphosphate synthase; GGPP,geranylgeranyl diphosphate; ...
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Báo cáo khoa học: Modular kinetic analysis reveals differences in Cd2+ and Cu2+ ion-induced impairment of oxidative phosphorylation in liver pot

Báo cáo khoa học: Modular kinetic analysis reveals differences in Cd2+ and Cu2+ ion-induced impairment of oxidative phosphorylation in liver pot

... mmsuccinate and different amounts of CdCl2 and CuCl2(0, 5 and 10 nmolÆmg protein)1). After 3 min of incubation,medium with mitochondria was collected, centrifuged at10 000 g for 1 min at 4 °C and ... & MooreAL (1994) The relationship between electron flux and the redox poise of the quinone pool in plantmitochondria. Interplay between quinol-oxidizing and quinone-reducing pathways. Eur J ... Atexcess amounts, the heavy metals cadmium and copperare toxic and carcinogenic [1]. The ability of cadmium and copper to accumulate in the bones, liver and kid-neys determines their toxicity....
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