... the purified (lanes 1, 3 and 5) and recombinant (lanes 2, 4 and 6) enzymes were used. Lanes 1 and 2, E. quadrivirgata enzyme; lanes 3 and 4, E. climacophoraenzyme; lanes 5 and 6, A. blomhoffii enzyme. ... discussed.Materials and methodsMaterials and biological samplesThree different species of snake, E. quadrivirgata,E. climacophora and A. blomhoffii weighing about 210 g(110 cm long), 270 g (130 cm long) and ... Elaphe quadrivirgata, Elaphe climaco-phora and Agkistrodon blomhoffii, and cloned their cDNAs.Each mature snake DNase I protein comprised 262 amino acids. Wild-type snake DNases I with Leu130...