Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp PCC 6803 in complex with heme pot

... Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme Masakazu Sugishima 1 , Catharina T. Migita 2 , Xuhong Zhang 3 , ... fer- redoxin; HO, heme oxygenase; heme HO, H O in complex with heme; Syn HO-1, HO-1 from Synechocystis sp. PCC 6803; HmuO, HO from Corynebacterium diphtheriae; HemO, HO f...

Ngày tải lên: 23/03/2014, 13:20

... stereospecificity for hydrogen removal from the C4 position of the nicotinamide moi- ety of NAD(P)H. In our binding model, the re-face of the nicotinamide ring is in front of the a-hydrogen atom of ... using insight ii (Biosym ⁄ MSI, San Diego, CA). Within this structure, two oxygen atoms of the C a car- boxylate are situated within hydrogen- and ionic-bond- ing distance of...

Ngày tải lên: 16/03/2014, 05:20

... Xiaocheng Gu 1 , Ming Luo 1,3 and Xiaofeng Zheng 1,2 1 National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, China 2 Department of Biochemistry and ... con- taining 0.4 mL of the respective crystallization solution. To fully saturate the enzyme binding site, GMP was added in a 4 : 1 molar ratio with respect to wild-type and mutant HGPR...

Ngày tải lên: 23/03/2014, 07:20

... Kristiina Kruus 2 , Juha Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finland Keywords ascomycete; ... Joly N, Madzak C & Mougin C (2002) Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation w...

Ngày tải lên: 14/02/2014, 18:20

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site in an extended ... gauge crystal quality before autoindexing and determining the optimum rotation range using strategy from within mosflm [35]. Crystals were found to have the symmetry of t...

Ngày tải lên: 14/02/2014, 19:20

... metal cofactor, respectively. SODs are grouped into four classes according to their metal cofactors: copper and zinc-containing SOD (Cu,Zn-SOD), iron-containing SOD (Fe-SOD), manganese-containing ... cambialistic SODs. In addition to the tertiary structures of metal-specific SODs [4], crystal structures of several cambialistic SODs have been reported, including those from Porphyromon...

Ngày tải lên: 14/02/2014, 22:20

... (2003) Crystal structures of human prostatic acid phosphatase in com- plex with a phosphate ion and a-benzylaminobenzyl- phosphonic acid update the mechanistic picture and offer new insights into inhibitor ... phytate-bound structure of AppA, even in the absence of sulfate ions. The side chain of the corresponding Glu212 bends out of the cata- lytic pocket of PhyK avoidi...

Ngày tải lên: 16/02/2014, 09:20

... DESC subfamily in complex with benza- midine. The proteinase domain of DESC1 exhibits a trypsin-like serine pro- teinase fold with a thrombin-like S1 pocket, a urokinase-type plasminogen activator-type ... the catalytic machinery and structures required for binding of the main chain of the substrate peptide and proper positioning of the scissile bond with respect to the cata...

Ngày tải lên: 19/02/2014, 00:20

... determination of the BcZBP protein at a resolution of 1.8 A ˚ , provides important clues towards understanding the enzyme function, including the identification of a zinc-binding site within a potential ... proteins. B. cereus thus provides a convenient model organism for studying proteins potentially associated with the pathogenicity of the highly infectious B. anthracis. The zinc-b...

Ngày tải lên: 19/02/2014, 00:20

... Thermoactino- mycetes vulgaris. Results The crystal structure of the Vibrio proteinase The obtained Vibrio proteinase crystals formed clusters of needles, which transformed into thin platelets within a ... ongoing mutagenic research in which single and combined amino acid substitutions aimed at increasing the stability of the Vibrio protein- ase are being tested. Elucidation of...

Ngày tải lên: 19/02/2014, 16:20