Báo cáo khoa học: Characterization of the interactions of the nephrin intracellular domain Evidence that the scaffolding protein IQGAP1 associates with nephrin potx

... 228–243 ª 2004 FEBS 243 Characterization of the interactions of the nephrin intracellular domain Evidence that the scaffolding protein IQGAP1 associates with nephrin Xiao Li Liu 1 , Pekka Kilpela ¨ inen 1 , ... control the loading of the GST proteins to the beads. X. L. Liu et al. Intracellular domain of nephrin FEBS Journal 272 (2005) 228–2...

Ngày tải lên: 23/03/2014, 13:20

... revealed the pres- ence of an intron in the 5¢-UTR of the genes. The nuc- leotide sequences of the cDNA and genomic clones of cardosins diverge after a perfect match of six bases. At the point of divergence, ... bp) is the only region of the cardosin A 5¢-flanking region that shares significant similarity with the corresponding region of the cardosin B ge...

Ngày tải lên: 30/03/2014, 09:20

... positions of the degenerated primers TRHR1, TRHR2, TRHR3, and TRHR4 are shown with the final PCR product. (B) 3¢-RACE. The 3¢-end amplified fragment of the xTRHR cDNA is shown. The positions of the two ... and R306) that are highly con- served in the other TRHRs and that interact directly with TRH. The additional potential glycosylation site (Asn19) in the N-term...

Ngày tải lên: 21/02/2014, 03:20

... to occur at the transcriptional level [11,12]. The induction of mEH in rats is coordinated with the induction of other xenobiotic metabolizing enzymes. For example, the same compounds that induce ... at the wandering stage late in the last larval stadium. At the prepupal stage, EH activity declined to a level equal to that in the early time of the stadium. Northe...

Ngày tải lên: 07/03/2014, 21:20

... during the lifetime of the cell. This field is still relatively new, but has come a long way in terms of establishing hypotheses and providing proof of concept and statistical evidence for many of them. ... in the 5¢-UTR of the N-ras oncogene, and demon- strated that it could form a G-quadruplex in vitro, and that the formation of the G-quadruplex led to a signifi-...

Ngày tải lên: 23/03/2014, 03:20

... screening of the genome of the hyperthermophilic bacter- ium Thermotoga maritima for ester-hydrolyzing enzymes revealed a protein with typical esterase motifs, though annotated as a hypothetical protein. To ... comparison of TM0336 with the amino acid sequences of the most significant hits in the blast search, as well as with the carboxylesterase Est2 of Alicycl...

Ngày tải lên: 23/03/2014, 09:20

... at their C-termini. In the ER, the presence of a higher pH allows detachment of the retrieved protein from the KDEL receptors [4]. The REDLK Keywords anticancer therapy; bacterial toxins; intracellular ... its site of action [2,5]. This implies that PEA may interact with the KDEL receptor in order to traffic from the Golgi to the ER. The plant ribosome-inactivat...

Ngày tải lên: 23/03/2014, 15:21

... comparison with 3-indolylacetonitrile, the rate of hydrolysis of 3-phenyl- propionitrile is increased by a factor of 330, and the K m value is reduced by a factor of 23. With the exception of ¯uoro, ... (5¢-ACAATTGATGATTCAACGCCCAAC 3¢). Using the BglII sites in the 5¢ overhang of AtNIT1-for and the 3¢-noncoding region of the cDNA, the AtNIT1 cDNA was inserted...

Ngày tải lên: 23/03/2014, 21:21

... characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of ... sequence identity with other proteins Optimal alignment of Cg-Clp2 with Cg-Clp1 and other GH18 family members revealed regions of significant identity, espec...

Ngày tải lên: 19/02/2014, 00:20

... 5¢-UTR of the mRNA [15,16]. However, com- parison of the sequence of the 5¢-UTR of human pan- creatic DNASE1 mRNA reported previously [15] with the human genomic sequence showed that the 5¢-terminal ... product, of which the 3¢ 380 bp were identical to exons 1–3 from the 5¢-end of the reverse primer DN+231 to the 5¢-end of the region where the sequence...

Ngày tải lên: 19/02/2014, 06:20