Báo cáo khoa học: Folding of epidermal growth factor-like repeats from human tenascin studied through a sequence frame-shift approach pdf

... factor-like repeats from human tenascin studied through a sequence frame-shift approach Francesco Zanuttin, Corrado Guarnaccia, Alessandro Pintar and Sa ´ ndor Pongor International Centre for ... quantitative analysis of the RP-HPLC profiles showed that the rates of disappearance of the reduced forms are similar but not identical (Fig. 4A) . A fit of experimental...

Ngày tải lên: 23/03/2014, 13:20

... cause transcriptional changes to accommodate the activation potency of the original signaling pathway at the cellular level. Abbreviations AU, approximate unbiased; EGFR, epidermal growth factor ... the potency and dynamics of EGFR signaling are adap- tively tuned via biochemical parameters such as affinity constants, catalytic activities, the concentration of the signaling mediators...

Ngày tải lên: 30/03/2014, 01:20

... tetrazolium bromide assay [36] on mouse CT26 (colon carcinoma), 4T1 (mammary gland), N2C (mammary gland) and TSA (mammary adenocarcinoma) and on human U87 (glioblastoma) cancer cell lines, as ... evolution approach, one DAAO mutant was identified that has increased activity at low O 2 and d-Ala con- centrations and a 10-fold lower K m for O 2 . We report on the mechanism of this DAAO va...

Ngày tải lên: 16/03/2014, 02:20

... mesenchymal cells via a biphasic activa- tion of RhoA and its downstream target ROCK; a rapid and transient elevation of RhoA-GTP levels which was detectable as early as 1 min after TGFb stimulation and ... adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc Natl Acad Sci USA 103, 9536–9541. 120 Papakonstanti EA, Kampa M, Castanas E &a...

Ngày tải lên: 07/03/2014, 01:20

... HGFA forward, 5¢-CCACTTGGATGAGAACGTGA-3¢; HGFA reverse, 5¢-ATGATGCCGTAGAGGTAAGC-3¢; MET forward, 5¢-TTGCCAGAGACATGTATGATAAAGAATACT-3¢; MET reverse, 5¢-TTGTCACTGGGGAAATGGAT-5¢; HGF ⁄ SF forward, ... 5¢-CAGAGCCTGCCAAGATCACAGATG-3¢; KLK2 reverse, 5¢-CCATTACAGACAAGTGGACCC CCA-3¢; KLK3 forward, 5¢-ATGACGTGTGTGCGCAAG TTCACCC-3¢; KLK3 reverse, 5¢-GATCCACTTCCGGTA ATGCACCACC-3¢; KLK4 forward, 5¢-AATCA...

Ngày tải lên: 23/03/2014, 07:20

... disordered (area 3) and dimer (area 4) lattices. (B) Zig-zag lattice (area 2), square lattice (area 5) and dimers (area 6). (C) Zig-zag lattice (area 1) and dimers (area 6). Scale bars: (A) 100 nm; ... sphaeroides.An AB C Fig. 1. Overview of typical crystals imaged with AFM (raw images). Areas of different crystal lattices are indicated by dashed lines. (A) Two types of zig-zag (areas 1...

Ngày tải lên: 18/02/2014, 18:20

... obtained from Amersham. Nycodenz was obtained from Nycomed Pharma. DAMP was obtained from Molecular Probes. All other chemicals were obtained from commercial sources and were of reagent grade. Animals ... method of Lowry et al. [45]. Galactosyltransferase was assayed as described by Beaufay et al. [46]. Acid phospha- tase was assayed as described by Trouet [47]. Nitrocellulose mem...

Ngày tải lên: 19/02/2014, 02:20

... 33150–33160. 36 Yamazaki A, Yamazaki M, Yamazaki RK & Usukura J (2006) Illuminated rhodopsin is required for strong activation of retinal guanylate cyclase by guanylate cyclase-activating proteins. ... Yamazaki A, Tatsumi M, Bondarenko VA, Kurono S, Komori N, Matsumoto H, Matsuura I, Hayashi F, Yamazaki RK & Usukura J (2010) Mechanism for the regulation of mammalian cGMP phospho...

Ngày tải lên: 06/03/2014, 00:21

... shown). An alternative explanation is that membrane PE is attached to membranes through a hydrophobic anchor that has been added to the protein post-translationally. A web-based (expasy) analysis of ... prepara- tions have been analyzed and regarded as a different peptidase, as these preparations have shown some physical and enzymatic features that are different from those of it...

Ngày tải lên: 07/03/2014, 06:20

... Gui- maraes PE, Ojopi EP, Paquola AC, Piazza JP, Nishiy- ama MY Jr, Kitajima JP, Adamson RE et al. (2003) Transcriptome analysis of the acoelomate human para- site Schistosoma mansoni. Nat Genet ... Kan- azawa H & Wada Y (2000) Luminal acidification of diverse organelles by V-ATPase in animal cells. J Exp Biol 203, 107–116. 20 Alderighi L, Gans P, Ienco A, Peters D, Sabatini A &am...

Ngày tải lên: 07/03/2014, 12:20