Báo cáo khoa học: Solution structure of Cu6 metallothionein from the fungus Neurospora crassa pptx

... [5–8]. Since the MTs have little or no repetitive secondary structure, their tertiary structure is dependent on the number and type of metal ions they coordinate. The MT from the fungus Neurospora crassa, isasingle domain ... Determining the deviation of the generated structure with a reduced number of NOEs from the published MT1 structure provides a measur...

Ngày tải lên: 23/03/2014, 13:20

... mecha- nism of transphosphorylation, which implies the A B Fig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-site residues of the 20 conformers of the solu- tion structure ... electrostatically with the other charged groups. In the NMR structures, this side chain of D40 is a short distance (£ 3A ˚ ) from the side chains of...

Ngày tải lên: 18/02/2014, 08:20

... fact, in the structures of Crt, the side-chain of Phe 25 is close enough to the amide proton of Lys38 to induce a ring current effect. Description of the toxin structure and potential surface The ... reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identification of the type and boundaries of the secondary structure eleme...

Ngày tải lên: 20/02/2014, 11:20

... [15]. The solution structure of the MBD of rat (r)MeCP2 has recently been determined [17]. The MBD adopts a wedge- shaped structure, composed of an antiparallel b-sheet on one face of the wedge ... [18]. The structure of the helical coil a 2 /a 3 allows us to interpret the consequences of the six mutations. As P153(152) and G162(161) are buried in the prot...

Ngày tải lên: 21/02/2014, 00:20

... similarity of the structures. The lowest-energy structure from the RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negatively charged patches of ... regrowth upon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generates new neurons throughout the lifespan of the...

Ngày tải lên: 07/03/2014, 10:20

... towards the interior of the protein. The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... was computed as the ratio of the s tandard deviations of the chemical shifts of the amide nitrogen and p roton nuclei. Results Determination of th...

Ngày tải lên: 07/03/2014, 16:20

... relative orientations of the a and b domains. In one struc- ture, the catalytic cysteines face each other; in the other, the catalytic residues of the a domain face away from the a¢ domain. The crystal structures ... mm of monomers at 30 °C. Most of the 1 H- 15 N HSQC signals of the dimeric form of PDI-bb¢ coincide with the signals of the monomeric form o...

Ngày tải lên: 16/03/2014, 04:20

... espectively. On the other hand, slide for the GC pair at the GC step that links the G stretch with the C stretch is rather high ()3.3 2 A ˚ ). The nature of the base stacking interaction in the iso d(GGGGCCCC) ... comprehensive understanding of the stereochemistry that govern the structures of 2 ¢,5¢ nucleic acids, we recently reported the Fig. 5. Stereo plot of...

Ngày tải lên: 16/03/2014, 18:20

... although the length of loop I is longer in short neurotoxins. TheNMRstructureofNTX-1isclosesttothatofthe a-cobratoxin crystal. The superposition of these two structures shows an RMSD value of 3.00 ... of the X-ray structure can be observed in the NMR structures but the length of the b-strand in loop I in solution is somewhat shorter than that of the crystal one (...

Ngày tải lên: 23/03/2014, 13:20

... Together with the 3 J NHa coupling constants and the secondary chemical shifts (Fig. 2A), the NOE pattern characterizes the local structure of the peptide. As already mentioned, the analysis of the chemical ... and by the random /,w-angle distribution in the Ramachan- dran plot of the experimental structures. The unambig- uous absence of an NOE between the H...

Ngày tải lên: 23/03/2014, 15:20