Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles docx

... Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles John den Engelsman 1 , Erik ... desmin-related myopathy; phosphorylation; SC35; small heat-shock p rotein; ubiquitin isopeptide ligase. aB-crystallin is a member of the family of small heat-shock proteins [1–3]. In...

Ngày tải lên: 23/03/2014, 13:20

... threonine phosphorylation of tau [20,21]. To determine whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together ... models of AD [8], suggesting that disruption of the association of these proteins could represent a poten- tial therapeutic strategy for the treatment of AD. Here,...

Ngày tải lên: 14/02/2014, 14:20

... details of these signaling events have yet to be investigated, many of the molecular interactions down- stream of the Eph and ephrin molecules appear to lead to the regulation of the cytoskeleton of ... unknown whether the two classes of effectors can bind to B ephrins simulta- neously or if the binding of one affects the binding of the other. We report h...

Ngày tải lên: 19/02/2014, 12:20

... densitometric measurements of the autoradiograms using the Scan Analysis software of Biosoft (Cambridge, UK). The calponin phosphorylation in the presence of F-actin was conducted under the same ... change in the binding of either calponin to the modified actin. Thus, the actin- stimulated phosphorylation of calponins we observed was not the result of a nons...

Ngày tải lên: 30/03/2014, 14:20

... the MARE [45–49]. The increased concentra- tion of endogenous heme may facilitate the binding of Nrf2, instead of Bach1, to the MARE to activate the MARE-dependent promoter, as reported for the mouse ... and HO-2 mRNA. The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal. The ratio...

Ngày tải lên: 19/02/2014, 05:20

... GST beads. The binding of the different proteins to GST–SF1f was quantified (bottom) as the fraction of the input protein (% of input) that was bound to the beads (the total input protein was ... dupli- cate. One per cent of the input was loaded to allow quantification by phosphorimaging of the fraction of the 35 S-labelled proteins that was bound to th...

Ngày tải lên: 19/02/2014, 07:20

... cytometry revealed that the inhibition of cell growth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig. 3B). In a total of seven separate determinations, ... examined whether iron loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of...

Ngày tải lên: 19/02/2014, 16:20

... concerted proton transfer to the leaving group of the substrate occurs. Proton transfer The requirement of an acceptor for the Oc proton from the catalytic serine is a critical component of the mechanism ... the carbonyl carbon of the substrate undergoes a nucleophile attack by the Oc of the catalytic serine, while the proton is shuttled to the catalytic...

Ngày tải lên: 20/02/2014, 23:20

... respectively. The affinity of keto- profen was almost the same of d-luciferin. In addition, the calculated E-value toward ketoprofen was approximately 20. These results suggest that LUC-H could catalyze the ... an ability to distinguish the absolute configuration of the starting material, the ee of the compounds in the two layers will increase. After the above ment...

Ngày tải lên: 07/03/2014, 10:20

... each desaturase and to depict, for the first time, the com- plete pathway for PUFA biosynthesis in a protozoon. Moreover, it would help to resolve an old controversy related to the isomeric nature of the PUFAs ... con- sidering the complexity of their life cycles, where they have to adapt to dramatic changes of temperature and morphology. It may be very interesting t...

Ngày tải lên: 07/03/2014, 12:20