Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx

... specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes Maria A. Juliano 1 , Darren R. Brooks 2 , Paul M. Selzer 3 , Hector L. Pandolfo 1 , Wagner ... & Juliano, M .A. (2001) S 1 subsite specificity of a recombinant cysteine proteinase, CPB, of Leishmania mexicana compared with cruz...

Ngày tải lên: 23/03/2014, 13:20

... c 552 cleaved of its signal peptide is approx- imately 9 kDa, whereas the uncleaved product has a mass of approximately 11 kDa. Maturation of single -cysteine holocytochromes There are natural examples ... and CcmB are not involved in heme transport in E. coli [44,45]. Notably, maturation of an AXXAH-containing variant b -type cytochrome c 550 in the present study indicat...

Ngày tải lên: 06/03/2014, 09:22

... basic (Arg) or acidic (Glu) amino acids. Position P1 was always occu- pied by Arg because TTSPs have an exclusive prefer- ence for substrates that contain this amino acid (or Lys) [2]. Amino acids ... (Shanghai, China). Aprotinin, leupeptin, AEBSF, soybean trypsin inhibitor, pepstatin, bestatin and E-64 were from Roche Diagnostics (Laval, Canada). EDTA, ortho-phenanthroline and hepa...

Ngày tải lên: 23/03/2014, 04:21

... Fragariaxananassa UGT78D1 A. thaliana UGT8 6A1 A. thaliana UGT8 7A1 A. thaliana UGT8 3A1 A. thaliana UGT8 2A1 A. thaliana UGT8 5A1 A. thaliana SbHMNGT S. bicolor UGT76D1 A. thaliana UGT76E1 A. thaliana S39507 ... thaliana OsSGT1 O. sativa UGT74F1 A. thaliana UGT74F2 A. thaliana NtGT2 N. tabacum UGT75C1 A. thaliana UGT75B1 A. thaliana UGT75D1 A. thaliana UGT84B1 A. thaliana UGT8...

Ngày tải lên: 28/03/2014, 23:20

... of amino acids towards the N-terminal, two amino acids at a time, to identify the amino acids that impeded cAMP stimula- CyaB2-GAFB-cAMP rPDE 2A- GAF-B-cGMP CyaB2-GAF -A- cAMP CyaB2-GAF-B-cAMP A ... the tandem GAF domain of cyaB1 in which only GAF B appears to mediate signalling to investigate ligand specification. In fact, the interacting amino acids, Arg256 and Thr258, a...

Ngày tải lên: 19/02/2014, 02:20

... efficient in catalyzing the transamination of a number of amino acids, including glutamine, phenylalanine, leucine, kynurenine, methionine, tyrosine, histidine, cys- teine, and aminobutyrate (Table ... I/glutamine transaminase K (EC 2.6.1.64; KAT-I) is a multifunctional enzyme. In vitro, the enzyme catalyzes the t ransamination o f several amino acids (e.g. glutamine, methionin...

Ngày tải lên: 07/03/2014, 16:20

... encom- passing almost only the CUB domain, and the longer variant containing the CUB domain and the final 30 residues in the C-terminal end of the growth factor domain. These splice variants are also ... revealing a remarkable increase in contact between the prostate carcinoma cells and the stromal cells, which is critical for prostate cancer progression and metastases [66]. As hum...

Ngày tải lên: 19/02/2014, 07:20

... enzyme- bound, adenine-lacking cobalamins for free adenosyl- cobalamin, an adenine-containing cobalamin through intermediary formation of apoenzyme. The function of reactivating factors is to release a tightly ... that reactivating factor mediates the exchange of the enzyme-bound, adenine-lacking cobal- amin for free, adenine-containing cobalamin toward a cognate dehydratase. In a...

Ngày tải lên: 16/03/2014, 05:20

... PR, Saccamo NA, Thadeio PF & Volkmann RA (1996) Posttranslational amino acid epimerization: enzyme-catalyzed isomeriza- tion of amino acid residues in peptide chain. Proc Natl Acad Sci USA 93, ... Morishita F, Nakanishi Y, Kaku S, Furukawa Y, Ohta S, Hirata T, Ohtani M, Fujisawa Y, Muneoka Y & Matsushima O (1997) A novel D -amino- acid- containing peptide isolated from...

Ngày tải lên: 18/02/2014, 11:20

... conformational changes [1–4], becoming a mediator of the intrinsic phase of apoptosis by its insertion into mitochondrial membranes, a process that culminates in the release of cytochrome c and activa- tion ... and the incision was closed, simulating thyroidec- tomy. Purification of BAX-DC (rBAX) protein Recombinant GST–BAX-DC (protein lacking the C-termi- nal 20 amino acid...

Ngày tải lên: 07/03/2014, 05:20