Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt

... HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling France ... screening a HCC cDNA expression library. We have identified the RIIa regulatory subunit of cAMP-dependent protein kinase (PKA) a...

Ngày tải lên: 23/03/2014, 13:20

... Miyazawa K, Tsubouchi H, Naka D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy- ama O, Takahashi K et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth ... Coomassie Brilliant Blue. The intensity of the band of the protease domain was quantified using bovine serum albu- min (BSA) as a standard. The enterokinase-treated pro...

Ngày tải lên: 15/02/2014, 01:20

... C-terminal adenylation domain A 4 again is predicted to activate l-arginine, displaying 60% identity to the characterized A- domain of MycC. Interestingly, A 1 and A 4 inherit a highly identical (90%) ... intramolecular nucleophilic attack of the L-hOrn 3 a- amino group onto l-haOrn 4 . Taking into account that the synthetases involved in the bio- synthesis of the...

Ngày tải lên: 16/02/2014, 09:20

... temperature. Assay procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition ... using the BioRad Protein Assay (BioRad, Espoo, Finland) with BSA (Sigma-Aldrich, St Louis, MO, USA) as the standard. Assay components Streptavidin-coated don...

Ngày tải lên: 18/02/2014, 14:20

... processing of the basal and elevated amounts of procaspase-8 in both control and IFN-trea- ted cells and there was an approximately two-fold increase in the amount of the p18 large subunit of active caspase-8 ... cells treated with the combination of IFNa and TRAIL, relative to TRAIL alone. In view of the cleavage of caspase substrates such as BID and...

Ngày tải lên: 19/02/2014, 06:20

... and pET-PDE1 as template. PDE1(Arg189–Thr620) was amplified using the primer pairs 5¢-GGGAATTCCATATGAGAGACAATA TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢ ,and PDE1(Lys321–Thr620) ... University) using Takara Taq polymerase (BioWhittaker) and 30 cycles of 30 s at 94 °C, 2 min at 58 °C and 5 min at 72 °C. For amplification, the primer pairs 5¢-GGGAATTC...

Ngày tải lên: 19/02/2014, 12:20

... minichromosomes. Structural alterations in nucleosomal organization caused by TSA treatment Changes in the acetylation status of histones by TSA treatment may cause changes in the organization of chromatin. Such altered ... inhibitors such as TSA may change the bulk acetylation pattern of histones, and in this way alter the structure of chromatin incorpor- ating them...

Ngày tải lên: 19/02/2014, 12:20

... North American Chapter of the ACL, pages 154–162. Joel Tetreault, Elena Filatova, and Martin Chodorow. 201 0a. Rethinking grammatical error annotation and evaluation with the Amazon Mechanical Turk. In Proc. ... Educational Applications, pages 28–36. Alla Rozovskaya and Dan Roth. 2010b. Training paradigms for correcting errors in grammar and us- age. In Proc. of 2010 Ann...

Ngày tải lên: 20/02/2014, 04:20

... recognition. In recent years, a number of studies have inves- tigated integrating emotions and music in certain media applications. For example, Ishizuka and Onisawa (2006) generated variations of theme ... RMSE(V) and 0.64 in RMSE (A) . Note that RMSE0.5 means that there is roughly one quarter (25%) error in the va- lence/arousal values as they range from [-1,1]. N...

Ngày tải lên: 20/02/2014, 05:20

... ids that existed in both answers, and AllIds is the number of different ids in both answers. We calculated the overall average agreement ratio (Total Avg) and the average of the best matches ... on the reward, and on the appeal of the task in question. 3.2 Quality experiments As an example of the post-processing of the data, we conducted some preliminar...

Ngày tải lên: 20/02/2014, 09:20