Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf

... characterization and the identification of three L. amazonensis proteins that bind in vitro to the telomeric G -strand (L. amazonensis G -strand telomeric proteins; LaGT1, LaG T2 a nd LaGT3). Binding activities ... to the limiting amount of telomeric proteins present in these extracts, binding reactions were done with a minimum of 1 lg of the semi...

Ngày tải lên: 23/03/2014, 13:20

... contains the zinc-bind- ing site, which faces into the deep cleft formed by the two subdomains connected at the base of the cleft. The hinge-bending motion observed upon inhibitor binding occurs ... zinc-binding motif) of His505 in the ACE2 sequence is very similar to the location of the catalytic histidines in both ACE and thermolysin, sug- gesting that His505 i...

Ngày tải lên: 20/02/2014, 01:20

... whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 with PCMPS in the presence of either UDP-GalNAc or UDP. To increase the sensitivity ... a major site of interaction with UDP-GalNAc, Cys214 is also involved in binding. PCMPS and DFP modification of the serine-mutants of GalNAc-T1 We also exam...

Ngày tải lên: 08/03/2014, 10:20

... osteopontin proteins; we there- fore concluded that the fish protein is probably the ortholog 19 of mammalian osteopontin. The weak simi- larity observed at the amino acid level indicates 20 that both ... induced in osteoblast-like VSa16 and chondrocyte-like VSa13 cells after 4 weeks of mineralization (Fig. 4), suggest- ing a role of OP-L protein 15 in the process of...

Ngày tải lên: 23/03/2014, 07:20

... divided these into two: 10% for training and the remaining 90% for test data. The training data is used to estimate K according to rank score, and test data is used to test the method using the estimated ... will include: (i) applying the method to other part -of- speech words, (ii) comparing the method with existing other automated method, and (iii) extending the method to fin...

Ngày tải lên: 30/03/2014, 21:20

... -RXXAFL- that extend the N-terminal sequence of NPFF in mammals and recent reports demonstrating the importance of a protein conver- tase, the subtilisin/kexin isozyme SKI-1, in the processing of prohormones ... shown that the degradation of synthetic SQA-NPFF, observed in mouse brain slices, produces only a low amount of NPFF [45] and that the degradation o...

Ngày tải lên: 31/03/2014, 07:20

... studies revealed that RGG3 of EWS binds mainly to the G-quadruplex. To test whether formation of the G-quadruplex is necessary for RGG3 binding, we assayed the binding of RGG3 with Htelo in the presence of ... and the G-quadruplex DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentia...

Ngày tải lên: 15/02/2014, 01:20

... revealed that both contained membrane-spanning regions anchoring the proteins to the inner membrane but not in the cytoplasm of the bacterium (data not shown), which is consistent with those of defined ... chal- lenge with viable bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of their outer mem...

Ngày tải lên: 18/02/2014, 14:20

... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal ... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin)...

Ngày tải lên: 18/02/2014, 16:20

... containing different domains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing ... secreted into the extracellu- lar matrices of the inner ear. In the otoliths, they are both localized in the ring-like structures. These find- ings identify for t...

Ngày tải lên: 18/02/2014, 17:20