Báo cáo khoa học: The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product potx

... The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product Masahiro Mizuno 1 , Takashi Tonozuka 1 , ... the space between loops II and III. The length of loop II of TVA II is very short and the cleft is open, which enables TVA II to bind pullulan efficiently. Loop II of TAA occupi...

Ngày tải lên: 23/03/2014, 12:20

... hydroxyl group onto the substrate, as observed in other members of the amidohydrolase superfamily [24,25]. However, as no structure of complexes with either the substrate, product or inhibitors ... performed on the hA- CMSD crystals using the same beamline, clearly indicated the presence of a Zn metal ion bound to the enzyme. Anal- ysis of the diffraction data se...

Ngày tải lên: 18/02/2014, 06:20

... 5¢-AGATCTTATTCAATGGTGT CGGGCTGAACC-3¢ anddigestedwithEcoRV and BglII. Resulting 210-bp fragment was ligated with the 1.5-kb HindIII-EcoRV fragment from pUSI2E(GD) and pUSI2E digested with HindIII and BglII to yield pUSI2E(a G ). ... A ˚ resolution by the molecular replacement method. The schematic view of the overall structure is shown in Fig. 3A. Theenzymeexistsasadimerofthea...

Ngày tải lên: 21/02/2014, 03:20

... First, the only structures of natural TPRs in the PDB (13 TPR-containing coordi- nate sets) consist of nonglobular, extended arrays of helices [9,10,13–21]. Second, the majority of these (11 structures) ... Monomer of NlpI, showing the rolled-up array of helices with the C-terminus folding within the curvature of the N-terminus. Helix num- bers are in brackets. N...

Ngày tải lên: 07/03/2014, 16:20

... n domains II, III and IV are either distorted or the access of a cation to the site is blocked by the p resence of a side chain of a b asic residue. I n case of the IIAB s ite (Fig. 2), the acidic ... residue in the present structure is s omehow halfway between the loop-in and the loop-out position of the bell pepper annexin. Fig. 1. The three-dimensional str...

Ngày tải lên: 17/03/2014, 03:20

... to the exo-loop of OXG-RCBH. The coordinates and structure factors have been deposited in the Protein Data Bank (PDB) (accession code 3A0F). The structure of XEG was compared with those of other ... from cleavage of XXXGXXXG. The structures of these products were X, XG, GX, XX, XXG, XXX and XXXG, as confirmed by MALDI-TOF MS combined with enzymatic treatments of...

Ngày tải lên: 23/03/2014, 05:22

... site in the cur- rent structure might be a product of an inability of the Fig. 1. Structure of PPWD1 and comparison with other Cyp structures. (A) The structure of the isomerase domain of PPWD1 ... backbone nitrogen of Glu485 with the oxygen of Gly551, the side-chain of Glu485 is nestled in a deep pocket of the enzyme (Fig. 3B). An oxygen of the...

Ngày tải lên: 23/03/2014, 07:20

... of the domain with the active site of the enzyme on one side and the Rieske center on the other side of the sheet (Fig. 3). Covering one side of the sheet are two Fig. 2. Crystal structure of PhnI. ... following the sequence.) Together with the extended loop, which extends 20 A ˚ from the center of the funnel, they form the base of the b subunit...

Ngày tải lên: 23/03/2014, 09:21

... different domains of IDH were searched for separately because of different domain orienta- tions found in other IDH crystal structures. The rotation and the position of the dimer of the small domains, ... structure of the dimeric apo form of TmIDH at 2.2 A ˚ . The R-factor of the refined model was 18.5% (R free 22.4%). The conformation of the TmIDH struct...

Ngày tải lên: 23/03/2014, 10:21

... (II) , the first tetrahedral intermediate of the cycle. The subsequent decarboxylation of II results in the central reaction intermediate, the a-carbanion- enamine of ThDP (III). Protonation of ... a-carbanion ⁄ enamine (III). Protonation of III yields hydroxyethyl-ThDP (IV), and the release of the second product acetalde- hyde completes the catalytic cycle of...

Ngày tải lên: 30/03/2014, 10:20