Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc

... a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a- like physiological activity Federico del Rı´o-Portilla1, Elizabeth Herna´ndez-Marı´n1, ... 2, using the CLUSTAL Xprogram,shows a clear cut separation of all the b Na-ScTxs from all the a Na-ScTxs. The a Na-ScTxs have identities of the order of 50% among themselves, the same being ... 4876–4882.46.Inceoglu,B.,Lango,J.,Wu,J.,Hawkins,P.,Southern,J.&Hammock, B.D. (2001) Isolation and characterization of a novel type of neurotoxic peptide from the venom of the South African scorpion Parabuthus transvaalicus...

Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

... signal of the preceding amino acid, correlating the amide HN and the Ca signals, correlating the amide HN and the Ca signal of the preceding amino acid, correlating the amide NH with the Ca and ... here the high-resolution NMR structure of the human C-domain in solution, and present dataon its dynamics. On the basis of the structural data,we have performed a mutational analysis of the C-domain ... retains its RF activity [15]. The combination of the humanM-domain and C-domain, in the absence of the N-domain, is able to bind to the mammalian ribosomeand to induce the GTPase activity of...

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

... by the Natural Sciences and EngineeringResearch Council of Canada, the Alberta Heritage Foundation forMedical Research, CanBiocin Ltd (Edmonton, AB), and the CanadaResearch Chair in Bioorganic ... structural basis of the inhibition of the antimicrobial activity of CbnB2 by the leader and to assist future analysis of the interaction of preCbnB2 with its ABC transporter protease, it isessential ... of the prebacteriocin tofully interact with the target receptor. These observationsare consistent with the maintenance of signiﬁcant antibac-terial activity for the prebacteriocin, but at a...

Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

... a helicase sep-arates the double-stranded DNA into single strands,and replication of the leading strand and synthesis of the Okazaki fragments is initiated by RNA primersmade by the specialized ... OttingResearch School of Chemistry, Australian National University, Canberra, AustraliaAll organisms replicate DNA by copying one strand (the leading strand) in a continuous manner, whereas the other ... residual restraint violations were energy minim-ized in a shell of water using the program opal with stand-ard parameters [36]. The Ramachandran plot was analyzedusing procheck -nmr [37].Secondary...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... introduction of paramagnetic probes into the interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemicalshift and paramagnetic relaxation enhancement data in conjunction with dynamic ... 0.088Ramachandran analysisMost favoured 58%Additionally allowed 31%Generously allowed 6%Disallowed 5% a The sum of all NOE violations larger than 0.015 nm was calcu-lated for each structure and ... representative NOE peaks. Cross-peakintensity was the only parameter that was allowed to varyduring the actual ﬁt. The heteronuclear NOE of a 1H-15Nspin pair is deﬁned as the ratio of the corresponding...

Báo cáo khoa học: Hexameric ring structure of a thermophilic archaeon NADH oxidase that produces predominantly H2O pot

... Signiﬁcantly, this structural feature of NOXtp is highly similar to that of valosine-containingprotein-like ATPase from Th. acidophilum, an archaealmember of the AAA family (ATPases associated with a ... Multivariate statistical analysis of NOXtp. (a) The average (AV) of 939 translationally, but not rotationally, aligned particles with end-on orientation and the 10 most signiﬁ-cant eigenimages ... showed that the C12 2A mutant had similar NADH and NADPH oxi-dase activity to that of the wild-type protein; however, the C4 5A mutant and the double C4 5A ⁄ C12 2A mutant had < 10% of the NAD(P)H...

Báo cáo khoa học: X-ray structure of peptidyl-prolyl cis–trans isomerase A from Mycobacterium tuberculosis potx

... The ﬁnal absorbance was estima-ted from e ach curve; the absorbance at each time point wassubtracted from that value. A plot of the natural logarithm of these differences vs. time was linear ... PpiA, in a structure- base d sequence alignment. All three structures show an rmsd of approxi-mately 1.6 A ˚ from that of Mt PpiA, when the C a atoms arecompared using a cut-off of 3.5 A ˚ (with ... of the B. malayi Ppi,theequivalentofAla118isFig. 4. Sequence alignment. The three structures most similar to MtPpiA were identiﬁed in a BLASTsearch and used for a structure- based sequence alignment...

Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

... X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranosideSanjeewani Sooriyaarachchi1, Wimal Ubhayasekera1, ... (hereafter referredto as GGal) is found naturally as the polar head group of plant glycolipids. An estimated 16.6% of the totallipids in runner bean leaves represents GGal [8], and a similar abundance ... unit, based on the characteristic linear shape of the electron density,and the presence of 0.2 m NaSCN in the crystallizationTable 1. Data collection and reﬁnement statistics.Data collection a Environment...

Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot

... group and within a van der Waals distance of a methine carbon atom (CHA) of the porphyrin ring. Inconsequence, Gln57 is capable of tuning the redoxpotential of the heme group [7,8].An insert of ... towards the surface of the protein and contribute to the overallnegative surface potential. The major negative patch,however, is created mainly by the side chains of Glu51, Asp86, and Glu89. The ... Additionally, there are two partiallyoccupied sulfate ions. One of them forms two hydro-gen bonds with the main chain nitrogen atoms of Asp74 (2.80 A ˚) and Ala67 (2.86 A ˚) and with a watermolecule...

Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in afﬁnity of transthyretin to T4 and T3 pdf

... Analysis of N-terminal amino acid sequencing The N-terminal amino acid sequence of recombinant TTRswere determined using an automatic Edman degradation atLa Trobe University, Australia, and ... was faster than that of albu-min, which is a typical characteristic of TTR of mostvertebrates found in nature [21].Native TTR in plasma exists in the tetrameric form. The molecular masses of ... MJ, Swan ID, Re´rat C & Re´rat B(1974) Structure of human plasma prealbumin at 2.5 A ˚resolution. A preliminary report on the polypeptidechain conformation, quaternary structure and...

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