Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc

... 495–522. Ó FEBS 2004 Dynamics of bacterial sHsps (Eur. J. Biochem. 271) 2503 Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins Nicolas Lentze 1 , J. Andrew Aquilina 2 , ... FEBS 2004 elution of standard proteins was completely independent of temperature (Fig. 1D). Temperature- and concentration-dependent oligomerization...

Ngày tải lên: 23/03/2014, 12:20

... depletion of the 14-3-3f isoform sensitized to cell death, whereas overexpression of this isoform delayed cell death. A combination of tandem affinity purification and liquid chromatography–tandem ... C2-ceramide- induced apoptosis With the aim of further analyzing the role of 14-3-3 proteins in apoptosis, an evaluation of the ability of proteins to bind and to be regulate...

Ngày tải lên: 06/03/2014, 22:21

... volume of 5 mL each of a solution of 3-phenylpropionitrile (181.5 mg) and 3-phenylpropionic acid (205.2 mg) in methanol (10 mL), and Tris/HCl buffer (990 mL, 70 m M , pH 8.5) were mixed, and 5 ... determined from the plot of ratio areas vs. ratio concentrations. One unit is de®ned as 1 lmol convert- edámin )1 . Determination of temperature and pH optimum of AtNIT1 Tempe...

Ngày tải lên: 23/03/2014, 21:21

... inhibition of PFK may not be physiologically relevant [14]. Temperature- dependent mechanisms of enzyme control may contribute to the regulation of PFK activity as the body temperature of hibernators ... skeletal muscle of the golden-mantled ground squirrel (Spermo- philus lateralis). Particular attention is paid to the effects of temperature on enzyme allostery and the infl...

Ngày tải lên: 19/02/2014, 16:20

... D esign and synthesis of a p otent c yclic ana- logue of the myelin basic p rotein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation for induction of experimental ... side chain of Arg78 (H g ) and Glu82 (H c1 )and the medium-range NOE between the side chains of Asp81 (H b ) and Arg78 (H g and H c )]. In addition, Glu82 i nteracts...

Ngày tải lên: 07/03/2014, 16:20

... composition and temperature influence on neurolysin and TOP hydrolytic activities in order to understand their unusual variations of specificity [5,6] and broad spectrum of hydrolysis of biologically ... different temperatures and at different salt concentrations [5]. Temperature dependence of the hydrolysis reaction rates of the substrates by neurolysisn and TOP The te...

Ngày tải lên: 23/03/2014, 21:21

... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinity for their target, along with small size a...

Ngày tải lên: 14/02/2014, 22:20

... structure of cN-II showing interfaces A and B and the Mg 2+ site. The inset shows the tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown. Table 2. Effect of point ... inosine and IMP remains unaltered, and the effect of the enzyme activators ATP and Mg 2+ is also unchanged (Table 1). Furthermore, by simultaneously measuring the rate...

Ngày tải lên: 15/02/2014, 01:20

... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1...

Ngày tải lên: 15/02/2014, 01:20

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... Homology model of HYD Js . (A) Docking of D-p-HPH to the HYD Js active site. (B) The SGLs of HYD Bp are shown as magenta lines and those of HYD Js are shown in green. The tran...

Ngày tải lên: 18/02/2014, 08:20