Báo cáo khoa học: Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris ppt

... Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris Aina Sola ` 1,2 , Hannu Maaheimo 2,3 , Katri Ylo¨ nen 3 , Pau Ferrer 1 and Thomas Szyperski 2 1 Department of Chemical ... study of amino acid biosynthesis and central carbon metabolism of the yeast P. pastoris. In the framework of this study, we have established the BDF 13 C-labelin...

Ngày tải lên: 23/03/2014, 12:20

... residues and have a common stem tetrasaccharide of xylose (xyl), two galactose units (gal) and a glucuronic acid residue (GlcA). The repeating disaccharide of HS is N-acetylglu- cosamine and uronic acid, ... 6-0 NNN 6-0 NN Fig. 1. Schematic of the four vertebrate syndecans. Syndecans-1 and -3 core proteins are larger than those of syndecan-2 and -4, and can bear both he...

Ngày tải lên: 15/03/2014, 23:20

... Using transcriptional profiling, the objective of this work was to gain a better understanding of the amino acid con- trol of gene expression. As our aim was to study the effects of short-term amino acid starvation, ... recognition of protein quality acts through the sensing of free circulating amino acids resulting from the intestinal digestion of proteins [4,5]. Anot...

Ngày tải lên: 07/03/2014, 03:20

... that require tRNA for amino acid activation, the potential for misrecognition of related amino acids has been investigated [9–13] and modulated by amino acid replacements and active site redesign ... stability of the canavany- lated species. The role of tRNA as a cofactor for aminoacylation in those aminoacyl-tRNA synthetases that require tRNA for amino acid activation...

Ngày tải lên: 18/02/2014, 13:20

... (Q9BQS6) and ODFP (Q14990). Table 1. Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues present in the flexible region of the ... of wild-type and mutant Hsp25 The binding of ANS and other hydrophobic probes to a protein enables the comparative determination of the Table 2. Summary of changes in st...

Ngày tải lên: 07/03/2014, 04:20

... [2–4]. Yeast eEF2 is a protein of 842 amino acids [5]. The protein is evolutionary conserved and the amino acid sequence is 66% identical and 85% homologous to the sequence of human eEF2 [5]. eEF2 is ... forms of eEF2. Fig. 4. Comparison of the amino acid context surrounding the puta- tive phosphorylation site in eEF2 from various fungi, plants and metazoans. The posit...

Ngày tải lên: 07/03/2014, 05:20

... equal volumes of a 1–10 l M solution of the digests and a saturated solution of a-cyano-4-hydroxycinnamic acid as a matrix in 50% CH 3 CN with 0.1% trifluoroacetic acid. Four microlitres of this mixture ... Phe31 and Ile33) play a critical role in binding to the 43-kDa protein. Role of amino acids in the 4-kDa peptide By alanine-scanning mutagenesis of the 4-kDa peptide, w...

Ngày tải lên: 17/03/2014, 03:20

... user of long-chain fatty acids making a brain-specific isoform intriguing. CPT1c was identified and cloned from in silico sequences as a highly homologous member of the CPT1 class of enzymes and ... fluctuations in the levels of blood glucose [13], fatty acids [12,14,15] and certain amino acids [16]. A linkage between fatty acid synthesis in the CNS and feeding behavior was u...

Ngày tải lên: 14/02/2014, 22:20

... 6. Control of the period and amplitude of Src oscillations by the activities of the activatory phosphatase RPTP and inhibitory kinase Csk. Dependence of the oscillation amplitude (A) and period (B) ... coexistence of oscillations (limit cycle) and a stable steady state implies subcritical Hopf bifurcation and the appearance of an unstable limit cycle. The unstable and...

Ngày tải lên: 18/02/2014, 11:20

... function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) Andreas H. Guse University Medical Center Hamburg-Eppendorf, Center of Experimental Medicine, Institute of ... the anomeric carbon of the ribose, which in the precursor NAD is linked to nicotinamide, and the amino ⁄ imino group at C6 of the adenine moiety [2]. Spectroscopic data [3] a...

Ngày tải lên: 20/02/2014, 01:20