Báo cáo khoa học: Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay doc

... Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay Laurence Deloffre 1 , Pierre-Eric ... carried out by capillary zonal electrophoresis [24]. Competition assay The ACE competition assay is based on the ACE activity assay using a simple radio assay for angiotensin-convert...

Ngày tải lên: 23/03/2014, 12:20

... found in frogs and fish, and homologs occur in invertebrates. All AP-2s have a highly conserved basic helix–span–helix DNA-binding and dimerization domain at their C-terminus, and a less conserved ... patterns of AP-2 molecules and functional implications The expression and function of AP-2 isoforms have been systematically analyzed during murine embryo- genesis and in studies...

Ngày tải lên: 16/02/2014, 09:20

... bap1 and bon1 pheno- types were reversed by loss-of-function mutations in SNC1, but also by loss-of-function mutations in EDS1, PAD4 and by nahG, encoding a salicylic acid (SA)-degrading enzyme ... cal- modulin-response genes and different types of senes- cence-associated and senescence-induced kinases, called SARK and SIRK, respectively [125]. SARK and SIRK share similar...

Ngày tải lên: 16/03/2014, 02:20

... Gly32, Ala41 and Leu65. On the basis of this, four point mutants L29D, G32D, A41D and L65D of ESAT-6 were generated. We studied complex formation between ESAT-6 mutants and CFP-10 by CFP-10 pull-down assays ... monocytes and macrophages [9], is quite sus- ceptible to trypsin. A similar stability profile for ESAT-6, CFP-10 and the complex towards lysosomal enzymes cathepsin L and...

Ngày tải lên: 30/03/2014, 11:20

... future assays. The effect of phosphatase inhibitors To prevent (in)activation of the enzymes by dephos- phorylation, phosphatase inhibitors were added before the production of cell-free extracts, and ... authors. Standardized enzyme assays for systems biology K. van Eunen et al. 760 FEBS Journal 277 (2010) 749–760 ª 2010 The Authors Journal compilation ª 2010 FEBS Measuring enzyme...

Ngày tải lên: 06/03/2014, 09:22

... PtdCho (50 mol%) and BMP (20 mol%). The standard incubation conditions were 37 °C for 30 min, and the enzyme assays were stopped by the addition of 50 lL methanol. Terminated enzyme assays were loaded ... chain and b chain, and the homodimeric isoenzymes b-hex- osaminidase B (HexB, bb) and b-hexosaminidase S (aa). Despite the a and b subunits being 60% identical in their pri...

Ngày tải lên: 07/03/2014, 12:20

... occurs by two different pathways. A conven- tional nonamyloidogenic pathway occurs via proteolytic cleavage of APP by a-andc-secretases and results in release of nontoxic soluble a-APP(s) protein and ... DNase I and used in RT/PCR. The reaction products were analysed by electrophoresis through 3% agarose and stained with ethidium bromide. As expected, 295-bp products of revers...

Ngày tải lên: 08/03/2014, 08:20

... incubated at 37 °C and analyzed by 13 C-NMR spectroscopy after 2 and 8 h. NMR assay of the IspC reaction using glycolaldehyde phosphate and hydroxyacetone as putative substrates Assay mixtures contained ... D 2 O and IspC (from E. coli, M. tuberculosis and A. thaliana, respectively) in a volume of 500 lL. The mixtures were incubated at 37 °C and analyzed by 13 C-NMR spectr...

Ngày tải lên: 16/03/2014, 06:20

... trajectories of differentiation and development by controlling chromatin archi- tecture and epigenetic memory, transcription, splicing, RNA modification and editing, mRNA translation and RNA stability. Sunday ... ligand-binding domain, a single transmembrane region and an intracellular signalling domain. TLRs are activated by var- ious stimuli that differ considerably in size, sh...

Ngày tải lên: 16/03/2014, 10:20

... Nieduszynski IA, Anderson K, Boocock J, Sandall PL & Weeks SD (2001) Characterization of oligosaccharides from the chondroitin sulfates. 1 H-NMR and 13 C-NMR studies of reduced disaccharides and tetrasaccharides. ... sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides Thomas N. Huckerby 1 , Ian A. Nieduszynski 1 , Marcos Giannopoulos 1 , Stephen...

Ngày tải lên: 16/03/2014, 14:20