Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

... E-cadherin is an important component of the cell–cell adhesion complex and required for the for- mation of epithelia in the embryo and the maintenance of the polarized epithelial structure in the ... causally involved in tumor progression, the understanding of these underly- ing intracellular events is of importance. In the present study, we explored t...

Ngày tải lên: 19/02/2014, 05:20

... Transport of the phosphonodipeptide alafosfalin by the H + /peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells Jana Neumann 1 , Mandy Bruch 1 , Sabine Gebauer 2 and ... intestinal (PEPT1) and renal (PEPT1 or PEPT2) epithelial cells. Absorption of intact di- and tri-peptides and related mimetics at the intesti...

Ngày tải lên: 23/03/2014, 12:20

... sorting of the Ntcp protein. Cysteine mutants Each of the eight cysteines, four in transmembrane domains, three in cytoplasmic or extracellular loops, and one at the beginning of the C-terminal ... cysteines. Tagging of Ntcp mutants by the FLAG Ò motif Todeterminewhetherthewild-typeandthemutantproteins are expressed and located on the surface of the oocytes,...

Ngày tải lên: 17/03/2014, 09:20

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... line), in the presence of 100 l M neurotoxin ( • , dotted line), in the presence of 200 l M neurotoxin ( , dotted line) and in the presence of neurotoxin...

Ngày tải lên: 14/02/2014, 19:20

... localize the ligand binding sites in the leukocyte b 2 integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi- mately 200-amino-acid intervening domain (I domain), which ... anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efficient inhibition of the Fig. 10. Specific binding of purified rec...

Ngày tải lên: 20/02/2014, 11:20

... derived from both C- and N-terminal sequences were synthesized and initially assayed in rat brain protein kinase C to identify which part of the entire protein maintained the in vitro effects described ... of the living cell, including acute in ammation, involve reversible phosphorylation of proteins. The number of protein kinases encoded by the human genome is es...

Ngày tải lên: 07/03/2014, 05:20

... 3D structure of McHr. (B) 1, iron-binding ligands of T. zostericola; 2, candidate iron-binding residues of McHr; 3, overlay of (1) and (2). (C) 1, amino acids of T. zostericola forming the O 2 -binding ... analysis modelled the putative haemerythrin to a four a-helix bundle, maintaining both the conserved haemerythrin positioning of the amino acid residues coordinating...

Ngày tải lên: 07/03/2014, 17:20

... the trans-side of the membrane. Moreover system y + L is inhibited by L -leucine in the presence, but not in the absence of Na + . The small inhibition of L -arginine in ux by L -leucine in the ... labelled arginine and at the end of the efflux experiments, were not different. The kinetic parameters of L -arginine in ux and efflux were calculated by...

Ngày tải lên: 08/03/2014, 02:20

... condi- tions, by the Jun N-terminal kinase, the P38c MAP kinase, the cyclin-dependent kinases 1 and 2 and the PDZ-binding kinase, resulting in changes in distribu- tion and stability of the protein [20–23]. ... translational efficiency by acting as binding sites for some regulatory proteins or by inhibiting the bind- ing or scanning of the translational machin...

Ngày tải lên: 22/03/2014, 16:20

... methionine and histidine side chains that coordinate the heme iron, and the cysteine side chain that forms a thioether bond between heme and protein. Also shown is the methyl group of the alanine of ... (histidine and methionine in each case), the cysteines that form thioether bonds to the heme, and the methyl group of the alanine of the C. fasciculata AX...

Ngày tải lên: 23/03/2014, 04:21