Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

... Bacterial IscU is a well folded and functional single domain protein Salvatore Adinolfi 1 , Francesca Rizzo 1 , Laura Masino 1 , Margie Nair 1 , Stephen R. Martin 1 , Annalisa Pastore 1 and ... here a structural characterization of this protein and demonstrate that E. coli IscU can be obtained as a recombinant well- folded protein. We demonstrate that our...

Ngày tải lên: 23/03/2014, 12:20

... Co-Talon TM . MS ana- lysis revealed that the band marked with an asterisk was recombin- ant Cdc45, the band above Cdc45 was heat shock protein 70 and the bands below were cytokeratin 1 and 9. ... antibody on formalin-fixed and paraffin-embedded tissues (Fig. 9). Antibodies against PCNA and Cdc45 stained malignant cells in a compar- able manner, e.g. on invasive-lobular mamma carci-...

Ngày tải lên: 19/02/2014, 00:20

... substrate for caspase 1; Ac-IETD-AMC is a substrate for caspase 8 and 10; Ac-LEHD-AMC is a substrate for caspases 2, 4, 5 and 9. Ac-DVPD-AMC, Ac-DPSD-AMC and Ac-ESQD-AMC are tetra peptide substrates representing ... follows: caspase 1, WEHD-AMC; caspase 2, VDVAD- AMC; caspase 3, DEVD-AMC; caspase 4, WEHD-AMC; caspase 5, WEHD-AMC, caspase 6, VEID-AMC; caspase 7, DEVD-AMC; ca- spa...

Ngày tải lên: 19/02/2014, 13:20

... TAT ATG T Weak 3 aa 2 ⁄ 4 55067 TCA ATG C Weak 58 aa 3 33 39% 60929 60933 60939 GTA ATG C TGC ATG T TCC ATG G G Adequate Weak Adequate 13 aa 33 aa 31 aa 3¢ 76 49% – 4¢ 56 61% 61041 GGA ATG T Adequate ... obvious retrieval signal is missing. The human phospholipases D1 and D2 are mainly associated with the plasma membrane or with the membranes of intracellular organelles although they lack...

Ngày tải lên: 19/02/2014, 17:20

... RNA helicase in translational repression is an RNA-mediated oligomer. Nucleic Acids Res 32, 1325– 1334. 5 Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y, Nagahama Y & Yamashita M (2003) ... J & Wickens M (2004) Mammalian GLD–2 homologs are poly (A) polymerases. Proc Natl Acad Sci USA 101, 4407–4412. 18 Nakanishi T, Kubota H, Ishibashi N, Kumagai S, Watanabe H, Yamashita M, Kashi...

Ngày tải lên: 07/03/2014, 05:20

... stromal cell-derived factor (SDF) -1alpha. Glycobiology 10, 21– 29. 34 Valenzuela-Fernandez A, Palanche T, Amara A, Mage- rus A, Altmeyer R, Delaunay T, Virelizier JL, Baleux F, Galzi JL & Arenzana-Seisdedos ... glycosaminogly- cans activates a p44 ⁄ p42 mitogen-activated protein kin- ase-dependent signaling pathway and enhances human Syndecan-4 is an auxiliary receptor for SDF...

Ngày tải lên: 16/03/2014, 18:20

... at approximately k = 490 nm for the carotinoid and ferredoxin-NADP + -reductase bands, and at k = 554 nm for the Cyt f a- band (Fig. 3). However, the absorbance maximum at k = 668 nm characteristic for ... spectrometric characterization of Pchl in the dimeric Cyt b 6 f complex was carried out by comparison of pigment standards protopheophytin (Pchl standard ) and protopheophorbide (Pch...

Ngày tải lên: 23/03/2014, 07:20

... radiation. Cancer Res 59, 3374–3378. 101 Kanaan A, Farahani R, Douglas RM, Lamanna JC & Haddad GG (2006) Effect of chronic continuous or intermittent hypoxia and reoxygenation on cerebral capillary ... and endothelial cells modulates tumour development and metastasis dissemination. The tumour microenvironment plays an important role in this cell interplay, and changes in its feat...

Ngày tải lên: 30/03/2014, 04:20

... Milne TA, Copeland TD, Levine SS, Lee JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA et al. (2004) Menin associates with a trithorax family histone methyltrans- ferase complex and with ... protein and its homolog p300 are general transcriptional co-activators that contain histone and transcription factor acetylation activities [30]. In addi- tion, CBP contains a number of...

Ngày tải lên: 16/02/2014, 14:20

... that all PAS-annotated A. thaliana proteins also contain a PAC motif, and conversely that all PAC- annotated A. thaliana proteins contain a PAS domain. Therefore, in the case of A. thaliana,thePASandPAC motifs ... Zhulin et al. ([2]), and Ponting and Aravind ([3]) observed that conserved motifs representative of PAS domains were ubiquitous in archaea, bacteria and eucarya, a...

Ngày tải lên: 19/02/2014, 12:20