Báo cáo khoa học: Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs doc

... by dimerization [6]. Figure 4 shows the peroxidase activity of iron(II)- and met-forms of HbI and HbIV. It is evident that the enzymatic activity decreases according to the order iron(II)-HbIV ... Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs A circular dichroism study Rosit...

Ngày tải lên: 23/03/2014, 12:20

... confident of three findings: (a) Polyols, amino acids and amino acid derivatives are ideal osmolytes, for they neither perturb the denaturation equilibrium nor affect the functional activity under native ... native conformational equilibria towards the most compact protein species within native state ensembles [32,37,38]. The variation in the effect of stabilizing osmolytes in modulatin...

Ngày tải lên: 07/03/2014, 00:20

... domain were also studied. The coincidence of the calorimetric and fluorimetric heat transitions in all cases indicated simultaneous, highly cooperative unfolding of the two domains. This cooperativity ... one-step irreversible native fi unfolded denaturation model (similar to the findings in Fig. 2A). This deviation may influence slightly differently the calorimetric and fluorimetric detec...

Ngày tải lên: 16/03/2014, 18:20

... work Automatic summarization evaluation can be broadly clas- sified into two categories (Jones and Galliers, 1996): in- trinsic and extrinsic evaluation. Intrinsic evaluation, such as relative utility ... effective- ness of a summarization system on other tasks. In this study, we concentrate on the automatic intrinsic summa- rization evaluation. It has been extensively studied in text su...

Ngày tải lên: 17/03/2014, 02:20

... analysis reveals similarities and variations in genetic programs controlling pollination ⁄ fertilization and stress responses in rice (Oryza sativa L.). Plant Mol Biol 59, 151–164. 63 Jain M, Nijhawan ... results provide evidence for a probable role of auxin-responsive genes in reproductive development and abiotic stress signaling in rice. Results and Discussion Identification and...

Ngày tải lên: 07/03/2014, 01:20

... solution, with unfolding of the protein over time leading to aggregation and progressive truncation from the C-terminus. Light scat- tering, MS, and size-exclusion chromatography data indicated ... Stein Eye Institute, University of California Los Angeles School of Medicine, Los Angeles, CA, USA 5 School of Biological and Biomedical Sciences, University of Durham, UK The verte...

Ngày tải lên: 16/03/2014, 18:20

... 19.9 is a tumour-associated antigen that is present in tissues that contain mucin or in the circulation, and that is located in the sialylated Lewis A blood group antigen [16-18]. The individuals ... HDL-high density lipoprotein. DISCUSSION We have made an investigation to try to vali- date a multivariate explanatory model of the diagno- sis of SCRC in Dukes’ stages I and II...

Ngày tải lên: 03/11/2012, 11:34

... located in the C-termi- nal region. The C-lobe of protein kinases mediates the interaction with activators, substrates and regulatory subunits, implying that the KIND domain, an atypical noncatalytic ... other KIND-containing proteins To examine the dendrite localization signal domains in v-KIND, we first investigated the subcellular localiza- tion of eight different Flag epitope-tagge...

Ngày tải lên: 14/02/2014, 19:20

... rhodamine uptake. Because MRP-1 is inhibited by MK571 and is an MRP that is relatively abundant in untreated ‘sensitive’ cells [4], it is a likely candidate for mediating innate resistance in these ... 555 nm and an emission wavelength of 575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching within the cells. Right: the various cell lines in...

Ngày tải lên: 18/02/2014, 13:20

... enzyme activity upon the apofiholo conversion is determined by cofactor structure, the process of dime rization is relatively indiscriminate in respect to structural modifications of PLP. Induction of structural ... unstable conformation. The detailed e xamination of the steps involved in subunit d issociation and reassociation will contribute to a better understanding of the di...

Ngày tải lên: 19/02/2014, 16:20