Báo cáo khoa học: Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir-binding site potx

... in the active site of the protease that is critical to inhibitor binding, L90M is distal to the inhibitor-binding site and is located near the dimerization interface. The kinetic parameters of ... crystal structure of the L63P/V82T/I84V mutant with indinavir [20]. Our structures of PR V82A and PR L90M showed opposite changes in the main chain at...

Ngày tải lên: 23/03/2014, 12:20

... On the basis of their crystal structures and the conserved amino acid residues (aa) of their active sites, these glutaminases are classified into a large group of bacterial penicillin-binding proteins ... region of the C-terminal domain; however, we failed to determine the structure of the intact glutaminase because the crystals exhib- ited heavy twinning. In the...

Ngày tải lên: 06/03/2014, 09:22

... In the absence of Thr, an outward shift of b-strands near the Thr-binding site (site 1) and a concomitant loss of the electron density of the loop region between b3 and b4 near the Thr- binding ... difference between the structures is that the electron density of the b3–b4 loop around the Thr-binding site is missing in TtAKb-free, and that b-strand...

Ngày tải lên: 18/02/2014, 08:20

... surface area of 6200 A ˚ 2 . The cofactor-binding site The cofactor binds on the periphery of domain I (Figs 3B,4) with the adenine ribose approaching the b1–a1 turn that carries the fingerprint motif ... [12]. In TbPDH, the alanine in this motif is replaced by glycine suggesting less steric in uence on cofactor binding. The adenine stacks against the Arg34 guanidi...

Ngày tải lên: 19/02/2014, 05:20

... containing two domains: an N-terminal five-bladed b-propeller and a C-terminal b-sandwich module. The active site is located in the centre of the b-propeller domain, in the bottom of a ‘funnel’. The binding ... IV and V blades and b-strands (1–6) from the internal b-sheet of the b-sand- wich domain. The last four amino acids of the native sequence at the...

Ngày tải lên: 06/03/2014, 00:21

... out of nine changes (including one A to V) occur in the e and g positions of HR1, and six out of nine changes (including two L to I and one I to V) occur in HR2 at the a and d positions. In contrast, ... close together. A region of about 270 amino acids would be located at the other end of the six-helix bundle between HR1 and HR2 in the postfusion sta...

Ngày tải lên: 08/03/2014, 08:20

... 45–58 in the former model and 40 , 49 and 55–58 in the latter model of SUMO-2 and the equivalents of ubiquitin. Although the sequences have only 18% identity, the protein folds of SUMO-2 and ubiquitin ... partic- ularly evident in the regions of 28–43 and 71–83, that correspond to the strand b2, the N terminus of the h elix a1, the helix a2, and...

Ngày tải lên: 16/03/2014, 18:20

... diverse family of proteins involved in the metabolism of amino acids, amino sugars and amino group containing lipids. A majority of them are key enzymes in the metabolism of amino acids. The reac- tions ... H3 and H19 and the antiparallel b strand S1 in the large domain and the helices H5 and H8 in the small domain are significantly different and could...

Ngày tải lên: 28/03/2014, 22:20

... described in (A) and (B). (E) Alignment of the primary structures of hinge-1 and hinge-2 of TvuCMBP and related solute-binding proteins. PfuMBP, P. furiosus maltodextrin-binding protein (amino acid ... for EcoMBP, and the bending and twist angles (35° and A B C D E Fig. 6. Structures of the hinge regions of TvuCMBP–a-CD and TvuCMBP–G4. (A, B) Comparison of...

Ngày tải lên: 29/03/2014, 23:20

... [3], a family of proteins generally involved in the transport of hydrophobic ligands [4]. The hypothesis of their involvement in the olfactory process, perception and transduction of the signal, ... onformational changes involving protein loops or backbone. T hus, the role of OBP in signal triggering remains unresolved. 3 Keywords: crystal str ucture; domain sw...

Ngày tải lên: 30/03/2014, 15:20