Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... activity of transgenic tobacco lines expressing the different ribosome- inactivating proteins and the in vitro polynucleotide– adenosine glycosylase activity of the respective proteins to- wards tobacco ... our results suggest that the in planta antiviral activity of some ribosome-inactivating proteins may rely on a direct mech- anism on the virus. In ad...

Ngày tải lên: 23/03/2014, 12:20

... response depends on the s~:rucrure of the data base. 5. Co~lexir~ of The domain: 6 .i Iu each type of interaction the complexity of the interaction depends both on the nature of the interac- tion ... User inforr~ation is essential in deter- minJ_ng expectations on the par~ of the user and the needs of the user. Within each type of interaction th...

Ngày tải lên: 17/03/2014, 19:20

... concentration of phosphinate and initiating with enzyme. Modality of inhibition The mode of inhibition of the slow-binding phosphi- nate was determined by examining the effect of varying each substrate ... 1, indicating simple binding at a single site for all the enzymes tested. Discussion An understanding of the kinetic and chemical mecha- nism of GspS and TryS involv...

Ngày tải lên: 07/03/2014, 04:20

... generalization, in order to not overfit the input sample. The input of the GRA consists of (i) a sam- ple set of document vectors with the correspond- ing values of , (ii) a maximum number of non-zero ... Formally, gets on input a set of vectors ; a corresponding set of values to be approximated; and a set of indexes of the ele- ments which are allowed to be non-z...

Ngày tải lên: 08/03/2014, 04:22

... accompa- nied by a reduction in the amount of cytosolic Sp1. In order to determine whether glutamine increases the binding of Sp1 to the SREBP-1a promoter region containing the Sp1-binding elements, we performed ... containing a mutated sequence of the SRE, pSQS containing the human SQS promoter, pSQS SREKO containing two mutated SREs, pLDLR containing the human LDL re...

Ngày tải lên: 28/03/2014, 22:21

... tasks. The advantage of using the extended set of fea- tures over the basic set is clearly illustrated in Table 2. The performance of the basic set on the chunking dataset is only at the level of the ... with incoming only, incoming and outgoing, and outgoing only ports, re- spectively, visible in the middle of Figure 1. 122 (a) Training (b) Tagging Figure 2: T...

Ngày tải lên: 16/03/2014, 20:20

... correspond- ing to the largest eigenvalue) is the direction in which the sum of the square of the projections of the row vectors of the matrix is maximum. In fact, the principal eigenvector of a graph ... This indicates that though 3 Binning is the process of dividing the entire range of a variable into smaller intervals and counting the number of obs...

Ngày tải lên: 22/02/2014, 02:20

... those with the C-terminal domain deleted is consistent with this (Figs 4 and 5). Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other localize according to the N-terminal ... the latter, indicating that in the absence of an N-terminal domain distinct localization is lacking. Finally, the Lsm1p Sm domain by itself (LsmDN1DCp) accumu- l...

Ngày tải lên: 07/03/2014, 02:20

... define further the features in the C-ter- minus of 4E-BP1 that are involved in its binding to raptor. The data for the other truncation mutants shown in Fig. 1C indicate that other regions of 4E-BP1 ... after treatment of cells with insulin [25]. Replacing its N-terminus with the N-terminal part of 4E-BP1 led to a marked increase in its phosphoryla- tion (even in t...

Ngày tải lên: 16/03/2014, 06:20

... times using NaCl ⁄ P i and the target protein was eluted by incubating the beads in 1· SDS buffer for 15 min at 55 ° C or boiling the beads in 1· SDS gel loading buffer for 10 min. Following SDS ... that the putative RhlB-binding microdomain can bind RhlB. We then used Blue Native-PAGE, a technique commonly employed in the separation of membrane protein complexes, in a stu...

Ngày tải lên: 29/03/2014, 21:20