Báo cáo khóa học: NUB1-mediated targeting of the ubiquitin precursor UbC1 for its C-terminal hydrolysis potx

... interact with a ubiquitin precursor UbC1 through their first UBA domain, resulting in the hydrolysis of UbC1 by an unidentified ubiquitin C-terminal hydrolase (UCH). The recruitment of UbC1 to the UCH ... linear ubiquitin precursor, UbC1, we hypothesized that NUB1 is a member of DUB family and has the enzymatic Fig. 3. C-terminal hydrolysis of ubiquitin...

Ngày tải lên: 23/03/2014, 12:20

... previously [18]. To assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in a het- erooligomer, the corresponding ... In the lower panels, the mean values and standard error of mean values of relative specific radioactivity of the different constructs are plotted. The nu...

Ngày tải lên: 07/03/2014, 00:20

... increment of its catalytic efficiency of 9.2 times for the phosphorylation of the adenine nucleo- tide. These observations with the double mutant indicated the existence of a cooperative effect for ... V mr values. With the exception of the Y239F mutant, which decreased its affinity for ATP compared with the wild-type, the other four mutants displayed a tende...

Ngày tải lên: 16/03/2014, 01:20

... membrane receptors of the import complex [41]. The positive resi- dues at the + 34 and + 39 positions of + 331A1 are indeed important for targeting of the protein to mito- chondria, as substitutions at these ... 0.7 mL of the supernatant was mixed with an equal amount of Nash reagent. The tubes were then incubated at 70 °C for 20 min, and the formaldehyde that wa...

Ngày tải lên: 18/02/2014, 16:20

... Laboratory of Biochemistry, Wageningen University, the Netherlands; 4 Centre for Molecular and Biomolecular Informatics, University of Nijmegen, the Netherlands; 5 Department of Biology, University of ... calculation of the fraction (f a ) and the effective quenching constant (K Q ) for the most accessible class of Trp chromophores [16]. The pH dependence of the...

Ngày tải lên: 21/02/2014, 00:20

... ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup- presses the release of the peroxide product. This may be one of the reasons why ApeSOD is less active in its Fe-bound form. It ... ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial pl...

Ngày tải lên: 14/02/2014, 22:20

... species. Here, the source of the native R2F-protein of the C. ammonia- genes ribonucleotide reductase were transformants from the reintroduction of the nrdF gene into the strain of its origin description ... RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We conside...

Ngày tải lên: 15/02/2014, 01:20

... N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11]. The regulatory domains of AK contain ... in the absence of Thr [20]. Therefore, a sim- ilar conformational change is expected for these two enzymes, depending on the presence or absence of Thr. In CgAK,...

Ngày tải lên: 18/02/2014, 08:20

... lgÆmL )1 ) for 6 h before the sampling for RNA preparation. Quantitative RT-PCR analysis for IFN-b mRNA was performed as described in (C). The IFN-b mRNA level was calculated relative to the level in the ... reporter) and cultured for 42 h, and then poly(I-C) (1 lg) was transfected into the cells for 6 h before the reporter assay as described in the Experimental proced...

Ngày tải lên: 18/02/2014, 16:20

... product. Therefore, the amount of product formed during the chase period is representative of the amount of ribozyme–substrate complex formed during t 1 . Note, however, that if k )1 $ k 2 , then ... investigated for the Pneumocystis ribozyme by measuring the pH depen- dence of the observed rate constant of the substrate- cleavage reaction. As seen in Fig. 4, the l...

Ngày tải lên: 18/02/2014, 18:20