Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II ppt

... elucidate the domains on the extrinsic 23 kDa protein involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II, we modified amino or carboxyl groups of the 23 kDa ... Interaction between the 23 and 33 kDa proteins (Eur. J. Biochem. 271) 971 Identification of domains on the extrinsic...

Ngày tải lên: 23/03/2014, 12:20

... histidine tag to facilitate the purification of the expressed fusion protein by a nickel-containing His- Bind column (Novagen, Gibbstown, New York, USA). The induction and isolation of the proteins ... amino acid res- idues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the pyruvate re...

Ngày tải lên: 23/03/2014, 04:20

... USA) containing the various agents were plated on the lawn in triplicate at 37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of growth inhibition in two axes, and the ... and 25% in pH 7.4 Dulbecco’s NaCl ⁄ P i containing 0.05% gelatin (DPBSG). Bacteria [10 lL containing 10 6 colony-forming units (CFU)] were inoculated into 190 lL reaction w...

Ngày tải lên: 18/02/2014, 14:20

... multiple domains are involved in FPRL1 interaction with its specific peptide agonists, with a prominent role for the sixth trans- membrane domain (TM) and the third extracellular loop in mediating the ... seg- ment spanning the C-terminal half of the first TM to the N-terminal two-thirds of the second TM and the second intracellular loop are highly conserved ex...

Ngày tải lên: 16/03/2014, 18:20

... and heparin-binding residues are present within the amino- terminal heparin-binding domain (ATD) of PKR. Thus, in contrast to the dsRNA-binding domain of PKR, its heparin-binding domains are located in ... regions led to a loss of heparin binding. The hep2 mutations are in a region between the conserved kinase subdomains VII and VIII within PKR’s catalytic domain. Althou...

Ngày tải lên: 23/03/2014, 13:20

... and the G-quadruplex DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming ... between the EAD and RNA polymer- ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelo binding of RGG3. To gain fur...

Ngày tải lên: 15/02/2014, 01:20

... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal ... experimental condi- tions. A number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tu...

Ngày tải lên: 18/02/2014, 16:20

... domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 (R1), the Glu-rich domain ... matrix proteins induce aragonite crystals [4–7]. We therefore exam- ined proteins contained in aggregates within the otolith matrix and identified a protein contained in t...

Ngày tải lên: 18/02/2014, 17:20

... catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre- cursor of all ... efficiency of uptake of b-amyrin from media is one of the reasons for the low conversion, it would be improved by in situ supply of the substrate through coexpres...

Ngày tải lên: 19/02/2014, 07:20

... tissue. Involved in G -protein signalling 24.3 Diacylglycerol kinase, epsilon (64 kDa) May be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during ... in the absence of the STAT2 DNA binding domain as revealed by real-time PCR To more quantitatively examine the expression of IFN- regulated genes in the ab...

Ngày tải lên: 19/02/2014, 07:20