Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... the infected female and male, with the vagina having a pH range of 4. 2–7.5 and the male urethra having a pH of 6.2–8 .4. The pH dependence of NG PBP 4 (pK a values of 6.9 and 10.1) therefore overlaps ... 6.9 and 10.1. Comparison of the enzymatic properties of NG PBP 4 with other DD -carboxypeptidases highlights both similarities and differences within these enzyme...

Ngày tải lên: 23/03/2014, 12:20

... using fasta [46 ] against Swissprot release 41 . 24 [47 ], TrEMBL release 24. 13 [47 ] and 138 completely sequenced genomes. Further screenings were performed against the NCBI non-redund- ant protein database ... pmol of tem- plate and 0.5 U of Pfu polymerase. The temperature cycles were 30 s at 94 °C, 1 min at 40 ° C and 2 min at 72 °C, repeated 10 times, followed by 30 s a...

Ngày tải lên: 19/02/2014, 17:20

... release more than one mole of adenine from each mole of ribosomes in 10 min and even up to 12 mol of adenines in 40 min (Fig. 4B). Base and position specificity of lamjapin in depurination of SRD RNA Synthetic ... lL of a solution of 0.2 M NaCl containing 100 lL of carrier tRNA and 40 lL of ethanol was added to the sample and kept at °C for 60 min. The mixture was c...

Ngày tải lên: 08/03/2014, 10:20

... NY. 41 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein uti- lizing the principle of protein- dye binding. Anal Biochem 72, 248 –2 54. 42 Runser ... performed using T-COFFEE [44 ]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between...

Ngày tải lên: 18/02/2014, 08:20

... capsid protein fold [26]. The distal domain is formed by an insertion of  230 residues (residues 133–367) between strands D and E and a smaller insertion of  50 residues (residues 40 4 45 8) between ... Adenovirus protein involved in virus internalization recruits ubiquitin -protein ligases. Biochemistry 41 , 142 99– 143 05. 26 Chelvanayagam G, Heringa J & Argos P (1992)...

Ngày tải lên: 19/02/2014, 06:20

... single band of 26 kDa was detected, as shown in Fig. 4B. Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of dithiothreitol, MgSO 4 and ... 26.5 °Cthan 4 °C. Semi-inactivation times were estimated to be 29, 24 and 12 h at temperatures of 4, 13 and 26.5 °C, respectively. Inhibition o...

Ngày tải lên: 21/02/2014, 00:20

... TATA and CAAT signals are present at position 41 40 ( )40 from the ATG) and 40 66 ()1 14) , respectively. In the 5¢ upstream untranslated region, we also found four inverted repeats (2 04 237 bp; 646 –715; ... acids. J. Biol. Chem. 277, 46 051 46 058. Ó FEBS 2003 Characterization of hazelnut seed lipoxygenases (Eur. J. Biochem. 270) 43 75 Biochemical and molecular characterizat...

Ngày tải lên: 21/02/2014, 00:20

... that of Arg358 in Tt-GluRS, and Ser461 substitutes Thr 444 (Fig. S7). In Mt-GluRS the pre- ceeding residue is Val460, which corresponds to Glu 443 of Tt-GluRS and His453 of Te-GluRS. Thus, Mt-GluRS ... of Mt-GluRS with chymotrypsin 0.1% (w ⁄ w) led to the formation of a limited number of protein fragments with five main species (bands M1-M5 of Fig. 5), of which M2 (27.5 k...

Ngày tải lên: 07/03/2014, 03:20

... specific activity of 13.5 UÆmg protein )1 . Corresponding pH optima and specific activities for syringaldazine and 2,6-DMP were pH 5.6 and 3 .4 UÆmg protein )1 and pH 4. 6 and 2.8 UÆmg protein )1 , respectively. In ... K m value of 0.03 m M and a V max of 16 .4 Umgprotein )1 . Corresponding values for syring- aldazine and 2,6-DMP under optimal conditions were 0.01 m...

Ngày tải lên: 07/03/2014, 15:20

... 20 04 Molecular and functional characterization of novel CRFR1 isoforms from the skin Alexander Pisarchik and Andrzej Slominski Department of Pathology and Laboratory Medicine, University of Tennessee ... majority of our isoforms were translated into proteins (Fig. 2) with the predicted size (Table 1). For example, band 4 (48 kDa) corresponds to the expected 47 .4 kDa for...

Ngày tải lên: 07/03/2014, 15:20