Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... the infected female and male, with thevagina having a pH range of 4. 2–7.5 and the male urethrahaving a pH of 6.2–8 .4. The pH dependence of NG PBP 4 (pKavalues of 6.9 and 10.1) therefore overlaps ... 6.9 and 10.1.Comparison of the enzymatic properties of NG PBP 4 withotherDD-carboxypeptidases highlights both similarities and diﬀerences within these enzymes, and suggests the possibility of ... [13a]. Characterization of PBPs in terms of enzymatic proper-ties, structure/function relationships, and catalytic mechan-ism [ 14, 15] provides useful information for understandingthe role of individual...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... using fasta [46 ] against Swissprot release 41 . 24 [47 ], TrEMBL release 24. 13 [47 ] and 138 completely sequenced genomes. Furtherscreenings were performed against the NCBI non-redund-ant protein database ... pmol of tem-plate and 0.5 U of Pfu polymerase. The temperature cycleswere 30 s at 94 °C, 1 min at 40 ° C and 2 min at 72 °C,repeated 10 times, followed by 30 s at 94 °C, 1 min at50 °C and ... Kent, UK) at 40 –60% of maxi-mum power. Magnesium chloride was added to a ﬁnal con-centration of 6 mm and DNA and RNA were hydrolyzedby incubation with DNaseI (4 lgÆmL)1) and RNase A (4 lgÆmL)1)...

Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

... releasemore than one mole of adenine from each mole of ribosomes in 10 min and even up to 12 mol of adenines in 40 min (Fig. 4B).Base and position speciﬁcity of lamjapin in depurination of SRD RNASynthetic ... lL of a solution of 0.2MNaClcontaining 100 lL of carrier tRNA and 40 lL of ethanolwas added to the sample and kept at °C for 60 min. Themixture was centrifuged (15 000 g for 20 min) and ... structure similar to theS/R domain of rat liver ribosomes. It was composed of astem and a GAGA tetraloop out of which A20 of SRDRNA corresponding to the A43 24 of rat ribosomal RNAwas ﬂipped [27]....

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... NY. 41 Bradford MM (1976) A rapid and sensitive method forthe quantitation of microgram quantities of protein uti-lizing the principle of protein- dye binding. AnalBiochem 72, 248 –2 54. 42 Runser ... performed usingT-COFFEE [44 ].Docking simulation of substrate on the active site of HYDJsThe substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were ... GD & Vanderdrift C (1976) Degradation of purines and pyrimidines by microorganisms. BacteriolRev 40 , 40 3 46 8.9 Dobritzsch D, Andersen B & Piskur J (2005) Crystalli-zation and X-ray...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... capsid protein fold [26]. The distaldomain is formed by an insertion of  230 residues(residues 133–367) between strands D and E and asmaller insertion of  50 residues (residues 40 4 45 8)between ... Adenovirus protein involved in virusinternalization recruits ubiquitin -protein ligases.Biochemistry 41 , 142 99– 143 05.26 Chelvanayagam G, Heringa J & Argos P (1992) Anat-omy and evolution of proteins ... penton base chimeraFEBS Journal 273 (2006) 43 36 43 45 ª 2006 The Authors Journal compilation ª 2006 FEBS 43 45Structural and biochemical characterization of a humanadenovirus 2/12 penton base chimeraChloe...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... single band of 26 kDa was detected, as shown inFig. 4B.Functional characterization of recombinant AKThe recombinant protein puriﬁed from the inclusionbodies and refolded in the presence of dithiothreitol,MgSO 4 and ... 26.5 °Cthan 4 °C. Semi-inactivation times were estimated to be 29, 24 and 12 h at temperatures of 4, 13 and 26.5 °C,respectively.Inhibition of AK by the speciﬁc inhibitor Ap5A and itseffect ... cultures of bacterial cells (XL1-Blue) transformed withpQE30-AK were induced with IPTG for overexpression of AK2 gene. Marked overexpression of AK2 protein wasnoticed but the recombinant AK protein...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... TATA and CAAT signals are present at position 41 40 ( )40 fromthe ATG) and 40 66 ()1 14) , respectively. In the 5¢ upstreamuntranslated region, we also found four inverted repeats(2 04 237 bp; 646 –715; ... acids.J. Biol. Chem. 277, 46 051 46 058.Ó FEBS 2003 Characterization of hazelnut seed lipoxygenases (Eur. J. Biochem. 270) 43 75Biochemical and molecular characterization of hazelnut(Corylus avellana) ... LOX primers CA4 and CA5 (matching,respectively, from base 948 8 to base 9520 and from base8366 to base 8387 of the sequence reported in Fig. 1) and Ó FEBS 2003 Characterization of hazelnut seed...

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... that of Arg358 in Tt-GluRS, and Ser461substitutes Thr 444 (Fig. S7). In Mt-GluRS the pre-ceeding residue is Val460, which corresponds toGlu 443 of Tt-GluRS and His453 of Te-GluRS. Thus,Mt-GluRS ... of Mt-GluRS withchymotrypsin 0.1% (w ⁄ w) led to the formation of alimited number of protein fragments with ﬁve mainspecies (bands M1-M5 of Fig. 5), of which M2(27.5 kDa), M4 (18 .4 kDa) and ... structure of the gene and properties of the expressed enzyme. Plant Mol Biol 58, 643 –658. 40 Gornall AG, Bardewill CJ & David MM (1 949 ) Deter-mination of serum proteins by means of the biuret...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... speciﬁc activity of 13.5 UÆmg protein )1. Corresponding pH optima and speciﬁc activitiesfor syringaldazine and 2,6-DMP were pH 5.6 and 3 .4 UÆmg protein )1 and pH 4. 6 and 2.8 UÆmg protein )1, respectively.In ... Kmvalue of 0.03 mM and a Vmax of 16 .4 Umgprotein)1. Corresponding values for syring-aldazine and 2,6-DMP under optimal conditions were0.01 mM and 4. 9 UÆmg protein )1, and 0.57 mM and 5.6 ... 255, 44 5 45 0.21. Bradford, M.M. (1976) A rapid and sensitive method for detectingmicrogram amounts of protein utilizing the principle of protein- dye binding. Anal. Biochem. 72, 248 –2 54. 22....

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... 20 04 Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee ... majority of our isoforms were translatedinto proteins (Fig. 2) with the predicted size (Table 1). Forexample, band 4 (48 kDa) corresponds to the expected 47 .4 kDa for pCRFR1f-V5; band 5 (43 kDa) ... withdeletion of exon 12 (AF369652); CRFR1g with deletion of exon 11, 27 basepairs of exon 10 and 28 basepairs of exon12 (AF369653); and CRFR1h with addition of a crypticexon (AF3 742 31) [15–18]....

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