Báo cáo khoa học: Inhibition of aryl acid adenylation domains involved in bacterial siderophore synthesis ppt

... structural information, by comparing models of the aryl acid binding pockets of the two domains. Results and Discussion Inhibition of DhbE with SAL-AMS and a set of new modified inhibitors In order ... of the SAL-AMS compound for aryl acid A domain inhibi- tion of SAL-capped siderophore producing pathogens [8]. Inhibition of aryl acid A domains bears the a...

Ngày tải lên: 23/03/2014, 11:20

... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl; and the fourth domain ... cobalamin-dependent MetS have revealed that it is a large, conformationally flexible pro- tein, consisting of four functional domains arranged in a linear manner. Each one of these domain...

Ngày tải lên: 19/02/2014, 05:20

... predominant in each of them. Carbaryl as a competitive inhibitor of AhR in yeast To elucidate the mechanisms causing inhibition of carbaryl in LMAX-RYA, a number of dose–response assays with increasing ... 3. Dose–response of BNF in the presence of increasing con- centrations of carbaryl in pLMAX-RYA. (A) Showing data relative to the maximal activity of BNF in th...

Ngày tải lên: 16/03/2014, 10:20

... in Drosophila, based on the precise comigration of the ansamycin-induced proteins with heat-induced proteins. Similar results were obtained with both ansamycins: geldanamycin Fig. 1. Ansamycins ... isoelectric focusing conditions. Anal Biochem 138, 144–155. 41 Zimarino V & Wu C (1987) Induction of sequence-spe- cific binding of Drosophila heat shock activator protein without protei...

Ngày tải lên: 30/03/2014, 20:20

... modifying h-toxin (per- fringolysin O), a cholesterol-binding, pore-forming cytolysin produced by Clostridium perfringens [18]. Ch and BCh are noncytolytic derivatives of h -toxin that bind specifically ... 30% abolishes their binding to plasma membranes [17–19]. This is in remarkable contrast to cell binding by filipin, another cholesterol-binding reagent. Filipin staining is significantly...

Ngày tải lên: 20/02/2014, 03:20

... H-line silkworms. Analysis of recombinant mouse IgG in cocoons To analyze secreted proteins in the sericin layer of the silk fibers, all proteins in the layer were dissolved in a buffer containing ... of mRNAs of the L-chain, H-chain and sericin-1 in MSG cells. Line L-chain a H-chain a Sericin-1 a Percentage of L-chain to sercin-1 Percentage of H-chain to sercin-1 L 2.4 ±...

Ngày tải lên: 23/03/2014, 04:20

... pathol- ogies. Abbreviations CBM, choline-binding module; CBP, choline-binding protein; CBR, choline-binding repeat; DEAE, diethylaminoethanol; MIC, minimal inhibitory concentration; t m , midpoint of thermal transition. 364 ... changes of C-LytA upon binding of these alkaloids are different to those induced by choline, suggesting a different mode of binding. In vitro inhibi- tion a...

Ngày tải lên: 19/02/2014, 05:20

... Zn 2+ in the p resence of ferrocyanide. We have ob tained evidence that isoalloxazine and t he surrounding amino acids are the binding site of the inhibitor. Clearly, the observation that binding of ... structural domains, each containing % 150 amino acids [11]. The C-terminal region includes most of the residues i nvolved in NADP(H) b inding, and the large cleft between the two...

Ngày tải lên: 19/02/2014, 16:20

... Peroxide-containing samples in presence of added Fe 2+ –EDTA; (h) Peroxide-containing samples in absence of added Fe 2+ –EDTA; (n) nonp hotolysed/non H 2 O 2 containing sample s in presence of added ... nactivation. Examination of the role of peroxide-derived radicals in enzyme inhibition To examine whether radical species were generated during the inactivation of GAPDH...

Ngày tải lên: 21/02/2014, 03:20

... above in vivo findings. PI3K/Akt is involved in PrP C -mediated MDR in gastric cancer In order to study whether activation of the PI3K/Akt signaling pathway played a role in PrP C -induced MDR in ... wavelength of 575 nm. (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells. (B) Adriamycin releasing index of gastric cancer cells. The r...

Ngày tải lên: 07/03/2014, 03:20