Báo cáo khoa học: Human-blind probes and primers for dengue virus identification Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences doc

... compilation ª 2005 FEBS Human- blind probes and primers for dengue virus identification Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences Catherine Putonti 1 , Sergei ... serotypes. Results Human n-mers In order to generate the set of all probes ⁄ primers that are present in the dengue virus...

Ngày tải lên: 23/03/2014, 11:20

... PCR template. For PCR, the tag-specific primer was used as the sense primer, and consisted of the 14-base tag nucleotides and 5¢ inosine nucleotides to increase the annealing temperature of the primers ... sequence of the PCR product has been obtained, check the tag to see whether its location is right after the last CATG site before the polyA signal in the...

Ngày tải lên: 07/03/2014, 00:20

... to describe situations and objects in the other (target) domain. Usage of the source domain’s terminol- ogy in the source domain is literal and is nonliteral in the target domain. Metaphor identification ... entendres and other forms of humor. Acknowledgments The authors wish to thank Tony Fader and Mark Yatskar for their insights and help with data, Bran- don...

Ngày tải lên: 07/03/2014, 22:20

... sources of errors in a preliminary error analysis. We therefore sug- gest three extensions to the algorithm which are on the one hand possible in the OM setting and on the other hand represent ... types of the edges. In order to be able to identify rarely occurring opinion targets which are not in the candidate list, they expand it by crawling the cast and crew...

Ngày tải lên: 17/03/2014, 00:20

... study, and informed consent was obtained from the patient. The COS-7 and 293T cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Isolation and induction of ... analyses of the relative levels of the indicated proteins after wes- tern blotting (as in D) were carried out. Simi- lar experiments were performed five times and...

Ngày tải lên: 23/03/2014, 03:20

... used for learning and the one for testing. SVM light was adopted for machine learn- ing, and RBF as the kernel. The parameters, i.e., the similarity threshold of DSIM classifier, gamma parameter of ... counterpart, with the direction opposite and the postfix “ -of attached to the label. This allows to follow the relations in reverse, increasing the flexibilit...

Ngày tải lên: 23/03/2014, 17:20

... immunoblot analysis with the anti-c-Myc IgG. The intensity of the band of the protease domain was quantified using the protease domain of the protein obtained from E. coli . Enzyme inhibition ... that of MSPL except for an insertion of five amino acids in the N-terminal cytoplasmic region and the C-terminal end following the protease domain, in which TMPRSS...

Ngày tải lên: 15/02/2014, 01:20

... reductase domain is located at the N-terminus and the predicted deaminase at the C-terminus of the bifunctional protein. In plants, orthologues of pyrimidine reductases of the riboflavin pathway ... Royal Society of Chemistry, Cambridge. Supporting information The following supporting information is available: Fig. S1. Construction of a synthetic gene for 2,5-dia- m...

Ngày tải lên: 18/02/2014, 18:20

... argues against a role for a3inthe formation of native MII binding sites, but may reflect the scarcity of these sites in the investigated brain tissues and/ or the formation of low affinity binding sites ... purpurascens 13 was again identified in a cloning approach making use of the high conservation of the 3¢ untranslated region and the intron preceding the sequ...

Ngày tải lên: 19/02/2014, 12:20

... node in the parse tree, and calculate the model score for each edge rooting at the node. The final output is the 1-best/k-best translations maintained by the root node of the parse tree. Since ... binarized in a head-out fashion 6 . A 5-gram language model was trained on the Xinhua portion of the Gigaword corpus in addition to the English part of the...

Ngày tải lên: 19/02/2014, 20:20