Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

... motif’, a structurally uncharacterized motif. Fig. 2. An overview of the PhKc domain structure. The first 296 residues of the subunit encode a protein kinase domain. The struc- ture of the constitutively ... these structures a C-terminal extension to the protein kinase folds back on the kinase domain and interferes with the substrate binding sites. In the case of...

Ngày tải lên: 19/02/2014, 17:20

... Hep Hep b | Hex 2 -Hex-Hep-AnKdo-ol | Hep | Hep a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS 2 experiments). b Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS 2 experiments). Fig. ... responsible for most of the steps in the biosynthesis of the OS portion of their LPS molecules...

Ngày tải lên: 08/03/2014, 02:21

... for the p eptide. NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson- ance dispersion of amide protons ( 0.7 p.p.m) and the lack of ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-40, and for fu...

Ngày tải lên: 16/03/2014, 16:20

... analysis of the side-view projections shows a maximal thickness of the PSII complex of about 9 nm including the densities on the lum- enal surface that has been attributed to the proteins of the OEC ... D1, and the extrinsic proteins of the oxyge n-evolving complex the 33 k Da, cyt c 550 and 12 k Da. The presence of the cyt c 550 in the PSII fraction w as c...

Ngày tải lên: 16/03/2014, 18:20

... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... chemical shift assignments in the 1 H-NMR and 13 C-NMR spectra and the characterization of the glycose components in the O-PS were determined from the appli- cation...

Ngày tải lên: 17/03/2014, 10:20

... rich domain at the extreme carboxy-terminal end. The GTPase domain is the most highly conserved domain within the members of the dynamin family. The functional roles of the various domains of dynamin ... for formation of short helices, transiently, and the chain as such is highly dynamic. Structure of the core of the oligomers Because of the...

Ngày tải lên: 23/03/2014, 11:20

... the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is proposed for the Hex5 glycoform in the Hep4-glycoform population of NTHi isolate 1209. Characterization of ... to the partial occurrence of PEtn attached to the phosphate group at O-4 of Kdo [11,12,15]. Structure of the core region of the Hep3-glycoforms. In the 1 H NMR spec...

Ngày tải lên: 08/03/2014, 08:20

... subjecting them to MALDI-TOF MS. Each of the fractions showed signals characteristic of the oligosaccharides. In the control group, the 0.2 m eluted fraction showed a signal with an m ⁄ z value of 2185 ... is one of the most difficult challenges and often requires the application of multiple analytical approaches [8]. MS has become a powerful analytical tool for the str...

Ngày tải lên: 22/03/2014, 17:20

... terminating at the active site near to the FAD cofactor. The presence of the long funnel is a charcteristic of a number of fla- voenzymes. The funnel of LGOX is composed mainly of the residues of the a-fragment. ... predicted the region of the respective fragments of mature LGOX. The regions and the theoretical molecular masses of the respective fragme...

Ngày tải lên: 23/03/2014, 05:22

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20]. In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1. To shed light on the pot...

Ngày tải lên: 15/02/2014, 01:20