Báo cáo khoa học: Factor VIIIa regulates substrate delivery to the intrinsic factor X-activating complex docx

... 2005 The Authors Journal compilation ª 2005 FEBS The intrinsic factor X (fX)-activating complex is com- posed of the enzyme (factor IXa; fIXa), the substrate (fX), and the cofactor (factor VIIIa; ... PN (2002) Coordinate binding stu- dies of the substrate (factor X) with the cofactor (factor VIII) in the assembly of the factor X activating complex on the...

Ngày tải lên: 23/03/2014, 11:20

... was bound in the substrate pocket, similar to the binding of dTTP to the D. melanogaster multi -substrate deoxyribonucleoside kinase [16], despite the funda- mental differences between the two structures. ... kDa (see Table 1). Therefore, it can be concluded that the nature of the base is insignificant for the tetra- merization effect. The next goal was to determine...

Ngày tải lên: 18/02/2014, 12:20

... respect to the lability of the zinc ions and that, despite the increased thermodynamic stability of the a domain with Zn 2+ over the b domain, the Zn 2+ sites in the a domain were shown to be more ... of the a domain of rhMT-1a, showing the connectivities of the four divalent metal cations to the 11 cystei- nyl sulfurs. The numbering of the cysteines (1–11 star...

Ngày tải lên: 19/02/2014, 00:20

... biosynthesis of sterols and fatty acids, heme ⁄ hemoproteins, NAD, and uracil [7,8]. The abil- ity to translocate ATP produced in the cytoplasm into mitochondria, and the ability to adjust the ... NADH generation, due to amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn- thesis takes place in the mitochondria. Because of the block in the re...

Ngày tải lên: 19/02/2014, 02:20

... protease Factor Xa (Boehringer Mannheim). The biotin-labeled Factor Xa was removed using a streptavidin gel and the free GST using the glutathione resin. To release the recombinant CD11b I domain the ... we studied the binding of ICAM transfectants to the integrins coated on plastic (Fig. 3B). All the ICAM transfectants, but not the wild-type L cells adhered to th...

Ngày tải lên: 20/02/2014, 11:20

... and then diluted with buf- fer to a final volume of 450 lL. The total volthour product was increased to approximately 80 kVh. The cathodic paper wick was immersed in 1% (w ⁄ v) dithioerythritol ... cleavage assays was rather haphazard. Thus, meprin and its substrate reper- toire may be studied in a complex biological context to identify physiologically relevant substrates. The...

Ngày tải lên: 07/03/2014, 06:20

... 15–20% of the total carotenoid content of the cell [29]. The absence of other carote- noids in the preparation suggested the selective binding of echinenone to the complex. To analyze whether echinenone ... shown to bind to the complex. In addition, the cyt b 6 f complex appeared to bind a caro- tenoid as well [14,16]. The existence of both pigments in a 1 : 1...

Ngày tải lên: 07/03/2014, 16:20

... 0.00125 to the overall accu- racy score. To allow the results from the two- and three-way decision tasks to be comparable (and to leverage the cost of creating the main task test set), the extended ... half of 65 the table shows the agreement between the two-way answer key and the annotations of the set of first judges; the bottom half is the same except using...

Ngày tải lên: 08/03/2014, 01:20

... by the LC-FTICR MS approach. Furthermore, the chromatographic separation of analytes prior to MS opens up the possibility to apply on-line fragmentation of components in complex mixtures. The information ... cor- responding to these two peptides were detected (Fig. 1b). The masses of the two intense signals to the right in the spectrum (Fig. 1c) matched those of the...

Ngày tải lên: 17/03/2014, 10:20

... [4–7]. Toc34 GDP interacts with the second G-protein in the Toc complex, Toc159, and simultaneously transfers the preprotein to Toc159. Toc159 action facilitates pre- protein translocation through the ... as Toc159, Toc64 and Toc34 (Fig. 3). The import yield of pSSU into protease treated chloroplasts dropped to approximately 20–30%, which corresponds well to the results des...

Ngày tải lên: 23/03/2014, 07:20