Báo cáo khoa học: Structural diversity of angiotensin-converting enzyme Insights from structure–activity comparisons of two Drosophila enzymes docx

... Structure-activity of Drosophila ACEsFEBS Journal 273 (2006) 362–373 ª 2005 The Authors Journal compilation ª 2005 FEBS 373 Structural diversity of angiotensin-converting enzyme Insights from structure–activity ... (1995) Race: a Drosophila homolog of the angiotensin-converting enzyme. Mech Dev 51, 157–168.22 Taylor CAM, Coates D & Shirras AD (1996) The Acergene of Drosophila codes for an angiotensin-converting enzyme ... Yiotakis A& Dive V (2003) Roles of the two active sites of somatic angiotensin-converting enzyme in the cleavageR. J. Bingham et al. Structure-activity of Drosophila ACEsFEBS Journal 273 (2006)...

Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus inﬂuenzae Identiﬁcation of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx

... similar levels of Neu5Ac [26] as well as glycine [20]. From the results (data not shown) of sugar (of LPS) andmethylation (of LPS-OH) analysis, ESI-MS (of LPS-OHand OS),1H NMR (of LPS-OH) and ... ESI-MS) of the Hep3-glycoforms men-tioned above.Characterization of OS fractions from lpsAmutant of NTHi isolate 1233 by NMRNMR data indicated the major Hep3-glycoform (Hex1) toconsist of a ... truncated versions thereof. Investigation of LPS from an lpsA mutant of isolate 1233 and a lic1 mutant of isolate 1209 was also performed, which aside from conﬁrming the functions of the gene products,...

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... representation of one coil of sbwAFP(red, residues G ly34 to T hr49) and TmAFP (blue, residuesAsn29ﬁGly41). Letters de note the ﬁve residues of on e of the threesides of s bwAFP o r s ix residues of on ... [53].Role of the TXT motif and water in activityExamination of the crystal structures of the insect AFPsalso revealed the presence of an array of water moleculesFig. 4. Comparison of the insect ... represent the addition of anadditional one, two or three b-helical c oils compared to theshorter isoforms. In the case of one sbwAFP isoform,named CfAFP-501, a detailed e xamination of t he structureand...

Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus doc

... visualization of most of themacromolecular compounds of the ASM on the samegel. N63 exhibits an important shift of approximately10 kDa, which represents a loss of apparent molecularweight of 14%. ... formation of the shell nacreouslayer, we have investigated the biochemistry of Nautilin-63, one of themain nacre matrix proteins of the cephalopod Nautilus macromphalus.After puriﬁcation of Nautilin-63 ... model.Furthermore, from the dozen primary structures of nacre proteins that have been described so far, most of them correspond to proteins of the pearl oyster or theabalone models. None of them were...

Báo cáo khoa học: Structural basis for poor uracil excision from hairpin DNA An NMR study pptx

... substantiallylower that that of t he U4-hairpin. This suggests that poorexcision of U from the U2-hairpin could be a consequence of its lower afﬁnity to for the enzyme. A caveat to thisinterpretation, ... majorgroove side of the DNA to make a ppropriate contacts in theactive site of the enzyme. Presumably, the potential energyrequired for these structural changes to occur before aproductive enzyme substrate ... determination of U1- and U3-hairpins by NMR andrestrained molecular dynamics. This paper describes theintricate details of three-dimensional structures of U1- andU3-hairpins, as derived from two- dimensional...

Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus inﬂuenzae serotype f Structural diversity observed in three strains ppt

... representativeofthediversitywithinthetypefcluster.Previous studies of LPS from H. inﬂuenzae haveresulted in a structural model consisting of a conservedFig. 4. Selected region of the two- dimensionalNOESY ... of LPS with Neu5Ac is widespreadamong H. inﬂuenzae strains [7]. However, levels of Fig. 5. Selected region of the two- dimensionalNOESY spectrum (mixing time 250 ms) of OSderived from LPS of ... FEBS 2003 Structural diversity of LPS in H. inﬂuenzae (Eur. J. Biochem. 270) 3167 Structural analysis of lipopolysaccharides from Haemophilusinﬂuenzaeserotype f Structural diversity observed...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... mainly builtup by residues from the C-terminus and consists of a two- stranded anti-parallel b-sheet composed of b2 andb9 and an a-helix from the C-terminus (Fig. 1A). The two subunits in the asymmetric ... structural characterization of numerous com-plexes of the human HPRT and several bacterial andprotozoan HPRTs have been undertaken [13–17]. Thestructure of human HPRT can be divided into two domains: ... 81potential substrates or regulatory ligands for enzymes in the human nucleotide metabolism. These wereselected as substrates, intermediates and products of other enzymes in the human nucleotide metabolism.The...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... widerange of spectroscopic techniques.Suitable methods for investigation of the secondarystructure and morphology of such structures, however,require the presence of large amounts of the oligomericstate, ... distribu-tion of 100 lM proinsulin C-peptide in thepresence of divalent Ca2+and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide in the presence of insulin (C) and of 100 lM ... population of monomerswith a hydrodynamic radius of approximately 16 A˚,which is in agreement with the results of the NMR.The addition of SDS to the C-peptide sample leads toformation of larger...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... is regulated in dual-ﬂavin enzymes like NOS is a topic of current interest.Characteristics of NOSNOS enzymes catalyze the NADPH- and O2-depen-dent conversion of l-arginine (Arg) to citrulline ... studies[17–22].NOS enzymes have novel featuresNOS are heme-thiolate enzymes and catalyze oxygenactivation by a mechanism similar to that of the cyto-chrome P450 (CYP) enzymes (Fig. 2). The ... assessment of the relative importance of conformational changerates versus rates of FMNH2formation in limiting elec-tron ﬂux through the CaM-bound NOS enzymes. Ingeneral, as the konand koffof...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... respec-tively [18]. All of the enzymes used in these syntheses(excluding MR) are available from Sigma-Aldrich (St.Louis, MO, USA) and the coenzymes are available from Melford Laboratories ... transi-tion state theory, the KIE arising from a full tunnellingreaction (described by Eqns 1–3) can be any value[10,46].Preparation of coenzymesThe methods of coenzyme deuteration are welldescribed ... to study the viscosity dependence of the rate of hydride transfer in GO [70,71]. These authors studiedvarious glycoforms of the enzyme (varying in theextent of glycosylation) [70] and also replaced...

Xem thêm