Báo cáo khoa học: Structural diversity of angiotensin-converting enzyme Insights from structure–activity comparisons of two Drosophila enzymes docx

... Structure-activity of Drosophila ACEs FEBS Journal 273 (2006) 362–373 ª 2005 The Authors Journal compilation ª 2005 FEBS 373 Structural diversity of angiotensin-converting enzyme Insights from structure–activity ... (1995) Race: a Drosophila homolog of the angiotensin-converting enzyme. Mech Dev 51, 157–168. 22 Taylor CAM, Coates D & Shirras AD (1996) The Acer g...

Ngày tải lên: 23/03/2014, 11:20

... similar levels of Neu5Ac [26] as well as glycine [20]. From the results (data not shown) of sugar (of LPS) and methylation (of LPS-OH) analysis, ESI-MS (of LPS-OH and OS), 1 H NMR (of LPS-OH) and ... ESI-MS) of the Hep3-glycoforms men- tioned above. Characterization of OS fractions from lpsA mutant of NTHi isolate 1233 by NMR NMR data indicated the major Hep3-glycoform (Hex1...

Ngày tải lên: 08/03/2014, 08:20

... representation of one coil of sbwAFP (red, residues G ly34 to T hr49) and TmAFP (blue, residues Asn29fiGly41). Letters de note the five residues of on e of the three sides of s bwAFP o r s ix residues of on ... [53]. Role of the TXT motif and water in activity Examination of the crystal structures of the insect AFPs also revealed the presence of an array of water molecules...

Ngày tải lên: 19/02/2014, 16:20

... visualization of most of the macromolecular compounds of the ASM on the same gel. N63 exhibits an important shift of approximately 10 kDa, which represents a loss of apparent molecular weight of 14%. ... formation of the shell nacreous layer, we have investigated the biochemistry of Nautilin-63, one of the main nacre matrix proteins of the cephalopod Nautilus macromphalus....

Ngày tải lên: 22/03/2014, 16:20

... substantially lower that that of t he U4-hairpin. This suggests that poor excision of U from the U2-hairpin could be a consequence of its lower affinity to for the enzyme. A caveat to this interpretation, ... major groove side of the DNA to make a ppropriate contacts in the active site of the enzyme. Presumably, the potential energy required for these structural changes to occu...

Ngày tải lên: 31/03/2014, 09:20

... representative ofthediversitywithinthetypefcluster. Previous studies of LPS from H. influenzae have resulted in a structural model consisting of a conserved Fig. 4. Selected region of the two- dimensional NOESY ... of LPS with Neu5Ac is widespread among H. influenzae strains [7]. However, levels of Fig. 5. Selected region of the two- dimensional NOESY spectrum (mixing time 250...

Ngày tải lên: 23/03/2014, 21:20

... mainly built up by residues from the C-terminus and consists of a two- stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two subunits in the asymmetric ... structural characterization of numerous com- plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17]. The structure of human HP...

Ngày tải lên: 15/02/2014, 01:20

... wide range of spectroscopic techniques. Suitable methods for investigation of the secondary structure and morphology of such structures, however, require the presence of large amounts of the oligomeric state, ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence...

Ngày tải lên: 18/02/2014, 04:20

... is regulated in dual- flavin enzymes like NOS is a topic of current interest. Characteristics of NOS NOS enzymes catalyze the NADPH- and O 2 -depen- dent conversion of l-arginine (Arg) to citrulline ... studies [17–22]. NOS enzymes have novel features NOS are heme-thiolate enzymes and catalyze oxygen activation by a mechanism similar to that of the cyto- chrome P450 (CYP) enzy...

Ngày tải lên: 18/02/2014, 11:20

... respec- tively [18]. All of the enzymes used in these syntheses (excluding MR) are available from Sigma-Aldrich (St. Louis, MO, USA) and the coenzymes are available from Melford Laboratories ... transi- tion state theory, the KIE arising from a full tunnelling reaction (described by Eqns 1–3) can be any value [10,46]. Preparation of coenzymes The methods of coenzyme deuteration...

Ngày tải lên: 18/02/2014, 11:20