Báo cáo khoa học: Template-independent ligation of single-stranded DNA by T4 DNA ligase doc

... of oxidative phosphorylation not only by the ANT but also by other components of oxidative phos- phorylation. Furthermore, we tested parts of the above hypothesis by determining the effects of ... adversely affect cellular function by direct effects on a variety of enzymes and induction of apoptosis [4]. Tight regulation of intracellular concentrations of free LCACs b...

Ngày tải lên: 19/02/2014, 05:20

... Proceedings of the COLING/ACL 2006 Main Conference Poster Sessions, pages 428–435, Sydney, July 2006. c 2006 ... Computational Linguistics 428 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 1 2 3 4 5 6 7 8 entropy offset 429 430 431 432 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0.55 0.6 0.65 0.7 0.75

Ngày tải lên: 20/02/2014, 12:20

... PHCP protein. Reagents Restriction enzymes, T4 DNA ligase and other reagents for DNA handling were purchased from Takara. All other chemicals used were of the highest grade commercially available. Acknowledgements We ... [16] structures. Cloning of the PHCP gene PCR with mixed primers PHcp01fw and PHcp01rv, using H. thermoluteolus chromosomal DNA as a tem- plate, gave a DNA fr...

Ngày tải lên: 06/03/2014, 00:20

... was accompanied by increased expression of BimEL, following activation of extracellular signal-regulated kinase. The aim of this study was to charac- terize the mechanism underlying hepatocyte apoptosis by ... showed only slight DNA binding (Fig. 3). Pretreatment with SP600125, an inhibitor of c-Jun N-terminal kinase, showed no effect on the DNA binding of c-Fos and c-Jun in...

Ngày tải lên: 06/03/2014, 00:21

... 3. Partial purification of plactin cofactor from bovine plasma Citrated bovine platelet-poor plasma (490 mL) was frac- tionated using the method described by Cohn et al. [25]. Most of the cofactor activity ... included to inhibit the feedback proteolysis of prothrombin by the generated thrombin). The reaction was stopped by the addition of an equal volume of acetic acid, and th...

Ngày tải lên: 07/03/2014, 00:20

... paths of each pair is calculated by employing Dynamic Time Warping algorithm. The input of the cal- culation is correlations between dependency re- lations, which are estimated from a set of train- ing ... level instead of word level. The experiment on TREC questions shows that our method significantly outperforms a density- based method by 50% in MRR and three state- of- the-art...

Ngày tải lên: 08/03/2014, 02:21

... order of prenominal adjectives in English. 2 Word bigram model The problem of generating ordered sequences of adjectives is an instance of the more general prob- lem of selecting among a number of ... entropy of a feature f which takes values V as the weighted sum of the entropy of each of the values V: H(D f ) = ∑ v i ∈V H(D f=v i ) |D f=v i | |D| Here H(D f=v i ) is the...

Ngày tải lên: 08/03/2014, 05:20

... were acquired by translating trained sentences by TDMT. 157 # of Linked Target Words # of Different Words in Target Language Mean # of Translation Words per Source Word Mean Context-freeness (# of Word ... level of MT quality achieved by each of the construction methods is compared in Table 2. Coverage of exact rules denotes the portion of sentences that were translated...

Ngày tải lên: 08/03/2014, 21:20

... of important biological responses, the significance of ERK signaling in terms of regulating miRNA expression is of great interest. To study the role of the ERK1 ⁄ 2 pathway in the regulation of ... plasmid pcDNA3-HA encoding Xenopus MEK1SDSE was supplied by E. Nishida (Graduate School of Biostudies, Kyoto University, Japan). A partial fragment of the 3¢ UTR of rat Bim was a...

Ngày tải lên: 16/03/2014, 01:20

... the presence of agmatine were assayed by measuring the production of l-ornithine. Urea was determined by a colorimetric method with a-isonitrosopropiophenone [28], and l-ornithine by the method of Chinard ... indica- ted by the numbers on the right side. The migration of the protein markers is indicated by pencil marks on the membrane. The arrow indicates the position of t...

Ngày tải lên: 16/03/2014, 12:20