Báo cáo khoa học: Variants of b2-microglobulin cleaved at lysine-58 retain the main conformational features of the native protein but are more conformationally heterogeneous and unstable at physiological temperature potx

... repeats 11 and 13 because repeat 11 is capable of binding IGF-II even in the absence of repeat 13 [20,22]. These data suggest that the failure to tether the tail of the extracyto- plasmic domain ... soluble forms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma- tion in the absence of ligand. Affinity cross-link...

Ngày tải lên: 18/02/2014, 08:20

... G (1996) The soluble catalytic domain of membrane type 1 matrix metalloproteinase cleaves the propeptide of progelatinase A and initiates autoproteolytic activation. Regulation by TIMP-2 and TIMP-3. ... amount of fully activated MMP-2 (62 kDa) and decreased the amount of the intermediate activated form (64 kDa) compared with cells attached to plastic (Figs 3A and 5). A...

Ngày tải lên: 18/02/2014, 11:20

... indicates that the M. jannaschii and C. glabrata enzyme are A-type reductases cataly- sing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate. Results Pyrimidine ... solution at room temperature, and a dual set of 13 C-NMR signals is therefore observed for each of the 13 C-labelled carbon atoms of the ribosyl si...

Ngày tải lên: 18/02/2014, 18:20

... living cells is naturally stochastic on the one hand, and influenced by the actual structural and functional state of the given chromatin domain on the other. Moreover, some of the p53 downstream genes ... 2006 The Authors Journal compilation ª 2006 FEBS 4693 DNA binding is underlined by the fact that most of the cancer-related point mutations of p53 are located i...

Ngày tải lên: 19/02/2014, 05:20

... amines. The finding that one but not the other of the two N-methyl derivatives of DAPY acted as an inactivator, would suggest that meta- bolism leading to inactivation occurs only at the propar- gylamine ... nucleophile resulting in inactivation. Two reported strategies in designing such inhibitors are the incorporation of either halogen or unsaturation at the b-posit...

Ngày tải lên: 19/02/2014, 16:20

... the reverse reaction, the oxygen atom of the free 5¢-OH group of one RNA fragment attacks the phosphorous of the cyclic 2¢,3¢-phosphate group of another, result- ing in ligation of the two fragments. ... cost of ligation is rather low and can be compensated for by the favourable reaction enthalpy of formation of the 5¢,3¢-phospho- diester bond via ring openin...

Ngày tải lên: 20/02/2014, 01:20

... dehydrogenases and methylenetetrahydrofolate dehydrogenases that are all Re-face specific at the carbon of the methylene group [27,28]. It has been calculated that the transition state conformation of methylenetetrahydromethanopterin and ... that the mass spectrum of F 420 formed from reduced F 420 by FprA-catalysed oxi- dation corresponds to that of a mixture of [5-...

Ngày tải lên: 20/02/2014, 03:20

... chunk–pair–distance statistics is the assumption that the strength of hid- den relationships between chunks can be covered by means of their collocation degree and the frequency of their relative positions ... user can either in- spect the content of a topic (i.e, the snippets or web pages) or activate the expansion of the graph through an on the fly computation of...

Ngày tải lên: 20/02/2014, 05:20

... stimulation in all three cell lines, and that this effect was reversed by gefitinib. The value for miR-125a-5p in the EGF group was set at 1, and the relative amounts of miR-125a-5p in the other ... array. We further demonstrated that miR-125a-5p inhibited migration and invasion of lung cancer cells. Moreover, miR-125a- 5p regulated the expression of several downstream...

Ngày tải lên: 07/03/2014, 00:20

... activity-related modulation of the pro-apoptotic proteins Bax and Bak and the phosphorylation- and proteasome-dependent downregulation of Bcl-2 after radiation treatment are required for the cell-death pathway, ... oligomerization, which is mediated by association of the death effector domain (DED) domains of the adaptor molecule, Fas-associated death domain (FADD),...

Ngày tải lên: 07/03/2014, 05:20