Báo cáo khoa học: Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolatesk docx

... from plants and insects M. Burow et al.2446 FEBS Journal 273 (2006) 2432–2446 ª 2006 The Authors Journal compilation ª 2006 FEBS Comparative biochemical characterization of nitrile-forming proteins ... characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolatesMeike Burow, Jana Markert, Jonathan Gershenzon and Ute WittstockMax Planck Institute ... (phenylacetonitri-le), and propyl isothiocyanate (1-isothiocyanatopropane)were procured from Sigma-Aldrich Chemie; benzonitrilewas from Merck (Darmstadt, Germany). Nitrile-forming proteins from plants and insects...

Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx

... The NOEpeaks between residues of the binding loop and that of the11±16 loop are also d ifferent from that of SGCI and itsvariant (Table 3).Qualitative evaluation of H±D exchange dataProton±deuterium ... properties o f t he amide protons of SGCI[L30R, K31M] are very similar to that of SGCI.Both the amide proton of Lys13 of SGCI and of SGCI[L30R, K31M] variant and Asp13 of SGTI arequicklyexchanging, ... difference between SGCI and SGTIis the differing conformation of their binding loops. Thecorresponding NOE pattern of SGTI is different from that of SGCI as well as from that recorded f or SGCI[L30R,K31M]....

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... Rao and Dr J.Josse for critical review of the manuscript, and Profes-sor W. Lo¨ffelhardt (University of Vienna, Austria) forhis assistance in N-terminal protein sequencing and some MALDI-TOF ... sPPases of photosynthetic bacteria,a polyphyletic and very diverse assembly of prokaryo-tes, are worth characterizing.An increasing body of biochemical and genetic evi-dence suggests that PPiplays ... UÆmg)1protein and recovery levels of 20–30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of puriﬁed preparations showed onlyone protein band of 20–22 kDa (Figs 1 and 2; supple-mentary...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... elements that are adopted only temporarily and ⁄ or in a fraction of the protein population.Recent data suggest that even proteins that adopt awell-deﬁned structure by conventional standards ... observed and described forlentiviral Tat [32,45–52] and Vpr [53–56]. A well-deﬁned and rigid tertiary structure of these proteins isobserved only in complexes with one of their ligands[51,52,57,58]. ... observation of multiplecontacts between pairs of residues that give rise to long-range NOEs. Indeed, several long-range NOEs wereobserved between T47 and Q61, between H72 and V78, and between H72 and...

Báo cáo khoa học: Mass spectrometric characterization of the covalent modiﬁcation of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt

... Fe -proteins or other proteins. Results and DiscussionSite-directed mutagenesis of the target arginine of dinitrogenase reductaseAn indication for the covalent modiﬁcation of onesubunit of ... shift of 541.06 m ⁄ z. Signals from thesingly and doubly charged b- and y-ion ser-ies, as well as ions from the fragmentation of the post-translational modiﬁcation, areindicated. The range of ... heterotrimeric G proteins, integrin,histones, and even DNA, as a regulatory process [27].Detection of ADP-ribosylation on proteins is oftenaccomplished by radioactive labeling of the donor mol-ecule...

Báo cáo khoa học: Comparative proteomic analysis identiﬁes proteins associated with the development and progression of colorectal carcinoma pot

... over-expression of the three proteins was signiﬁ-cantly associated with the genesis and progression of CRC. The functional implications of the alterations inthe levels of these proteins are discussed ... and metastasis-associated proteins in CRC. The results sug-gest that RhoGDI, S100A9 and LASP-1 may play animportant role in the development and progression of CRC. Further functional and clinical analysis of the proteins ... hand and cytotoxic ⁄ cytostatic activities against bacteria,fungi and tumour cells on the other hand [23].Previous studies have reported that S100A8 and S100A9 are frequently co-expressed, and...

Báo cáo khoa học: "Implementing a Characterization of Genre for Automatic Genre Identification of Web Pages" pot

... upgrade of existing genres and the creation of novel genres. The change of genre repertoire and the creation of new genres were well illustrated by Crowston and Williams (2000) and Shepherd and ... generated from specific needs and requirements of the web. Crowston and Williams (2000) have traced this evolution through a manual qualitative survey of 1000 web pages. Shepherd and Watters ... assumption that a document can be assigned to only one genre. In this paper, we propose a tuple representation that maps onto the theoretical characterization of genre suggested above and that...

Báo cáo khoa học: "A Descriptive Characterization of Tree-Adjoining Languages (Project Note)" ppt

... languages was obtained by Thatcher and Wright (1968) and Doner (1970) who established that the CFLs were all and only the sets of strings forming the yield of sets of finite trees definable ... Doner's and Thatcher and Wright's proofs that the recognizable sets of trees are char- acterized by definability in wSnS lift directly to a proof that the recognizable sets of three- dimensional ... are exactly the class of tree manifolds generated by TAGs if one re- laxes the usual requirement that the labels of the root and foot of an auxiliary tree and the label of the node at which...

Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt

... Dolce1,Ferdinando Palmieri3 and Loredana Capobianco21 Department of Pharmaco-Biology, University of Calabria, Arcavacata di Rende, Cosenza, Italy2 Department of Biological and Environmental Sciences and ... Dolce,Department of Biological and EnvironmentalSciences and Technologies, University of Salento, 73100 Lecce, Italy; Department of Pharmaco-Biology, University of Bari, 70125Bari, Italy; Department of Pharmaco-Biology,University ... family of transport proteins that shuttlemetabolites, nucleotides and cofactors across the inner mitochondrial mem-brane. The genome of Drosophila melanogaster encodes at least 46 mem-bers of...

Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... protein inE. coli and studied its substrate preferences, the factorsinﬂuencing its activity and the nature of suitable metalcofactors and inhibitors in vitro.Materials and methodsChemicalsThe ... mito-chodrial AOX activity and proposed that the effect of organic acids could be due to the fact that they chelate metalcations. We did observe an inhibitory effect of both citrate and malate on PTOX ... comparison betweenAOXs and the IMMUTANS gene product allowed areassessment of the amino acids that are likely to havefunctional involvement in AOXs [6] and suggested that theIMMUTANS gene...

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