Báo cáo khoa học: Prediction of coenzyme speciﬁcity in dehydrogenases ⁄ reductases A hidden Markov model-based method and its application on complete genomes doc

... Prediction of coenzyme speciﬁcity in dehydrogenases reductases A hidden Markov model-based method and its application on complete genomes Yvonne Kallberg1,2 and Bengt Persson1,21 IFM Bioinformatics, ... ﬁfthmajor group, zinc-containing alcohol dehydrogenases, has almost as many members as the SDRs (Table 2).Transmembrane regions A number of dehydrogenases and reductases are mem-brane-attached. ... domain’, ‘FAD ⁄ NAD(P)-bindingdomain’ and ‘Nucleotide-binding domain’. The dataset wasscrutinized and only proteins utilizing FAD or NAD(P) in a typical manner were used, i.e. only selecting sequenceswith...

Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc

... reconstruct the lysine-degradation pathway in P. aeruginosa using EC number information. As a result, we identiﬁed PA0266 as a putative 5-aminovalerate aminotransferase(EC 2.6.1.48) and PA0265 ... the lysine-degradation pathway of P. aeruginosa, because thispathway contains many missing enzymes and ourunderstanding of the detailed enzymatic behavior in this pathway is far from complete. ... 2267pyridoxal 5’-phosphate via a transamination reactioncatalyzed by PA0266. Therefore, we concluded thatPA0265 is glutarate semialdehyde dehydrogenase and PA0266 is 5-aminovalerate aminotransferase.DiscussionHere,...

Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt

... frombacteria and transferred to a new Petri dish and incu-bated overnight with medium containing gentamicin.Bacterial growth of each strain was assessed by viablecounts, both during initial infection ... prokaryotic evolution has created a new way to take advantage of an evolutionary ‘novel’eukaryotic 14-3-3 protein family, using them as a necessary cofactor to activate lethal bacterial toxins,but ... Delineation of exoenzyme S residues that mediate theinteraction with 14-3-3 and its biological activityLubna Yasmin1,*, Anna L. Jansson1,*, Tooba Panahandeh1, Ruth H. Palmer3, Matthew...

Báo cáo khoa học: Complementation of coenzyme Q-deﬁcient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt

... order and times of elutionwere approximately CoQ2(9 min), CoQ2H2(11 min),decylQ (17 min) and decylQ2(22 min). The area undereach peak was integrated and compared with standardscontaining ... h, at which point it had reached A 600 0.8 and already been sta-tionary for several hours and would remain that way for a number of days (squares). At the point indicated by the arrow, a mixture ... rates of mitochondrial respiration as a function of CoQ2(B), decylQ (C), A1 -Q2(D), A2 -Q2(E), A3 -Q2(F), A4 -Q2(G), A5 -Q2(H) and A6 -Q2(I). Data are the mean ± standard deviation of...

Báo cáo khoa học: Structure of coenzyme F420H2 oxidase (FprA), a di-iron ﬂavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O ppt

... and a C-terminal ﬂavodoxin-likedomain (residues 253–404) containing FMN. Twomonomers assemble via a head-to-tail arrangement,such that the b-lactamase and the ﬂavodoxin domainsface each other, ... contains both irons in a similar position and an occupancy as found in the reduced state. Addition-ally, Glu85 and Asp87 adopt the conformation of thereduced state, and the remote metal-binding ... FMN and modeling of F420H2The conformation and binding characteristics of FMNare nearly identical in all of the analyzed structures of F420H2oxidase but also in comparison to those of other...

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... transaminase and diminishes upon forma-tion of pyridoxamine after reacting with amino groupdonors (the half reaction of the overall transaminase-mediated reactions). The spectrum of hKAT-I ... hour.Among the 12 a- keto acids capable of functioning as aminogroup acceptors for hKAT-I, a- ketobutyrate, mecapto-pyruvate and oxaloacetate showed no substrate inhibitionat saturating concentrations, ... brains [14–16]. In addition, KYNA is involved in maintaining physio-logical arterial blood pressure. In rats, the region of therostral and caudal medulla in the CNS plays an importantrole in...

Báo cáo khoa học: "Prediction of Learning Curves in Machine Translation" ppt

... weights wjare estimated from data. Thetraining data for ﬁtting these linear models is ob-tained in the following way. For each conﬁguration(combination of language pair and domain) c and test ... there are more anchor points than pa-rameters, and also in view of the combination of inference and extrapolation in Section 6.7Curves on different test data for the same training conﬁgu-ration ... Since ad-hoc manual translation canrepresent a signiﬁcant investment in time and money, a prior assesment of the amount of training data required to achieve a satisfac-tory accuracy level can...

Báo cáo khoa học: "Prediction of Thematic Rank for Structured Semantic Role Labeling" doc

... continuation argument of a i, 6) a iCAaj: a iisthe continuation argument of a j, 7) a i= a j: a i and a jare labeled as the same role label, and 8) a i∼ a j: a i and a jare labeled as the ... gold parsing data and 95.14% accuracy on Charniak automatic parsing data.Most existing SRL systems divide this task intotwo subtasks: Argument Identiﬁcation (AI) and Semantic Role Classiﬁcation ... our method can yield signiﬁcant improvement over a state -of- the-art SRC baseline, achieving 0.93% and 1.32%253absolute accuracy improvements on hand-crafted and automatic parsing data.2 Prediction...

Báo cáo khoa học: Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc

... TSSK3.AbbreviationsAGC, containing PKA, PKG, PKC kinases family; CaMK, calmodulin-dependent protein kinase family; GA beads, glutathione agarose beads;GST, glutathione S-transferase; HA, haemagglutinin ... Bucko-Justyna et al. Characterization and regulation of TSSK3Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependentkinase-1-dependent signallingMarta ... membranelocalization of PDK1. As TSSK3 consists essentially of a kinase domain [2], it is conceivable that in cells otheradaptors ⁄ effector(s) may be necessary for maximumactivation and ⁄ or activation...

Báo cáo khoa học: Predicting the substrate speciﬁcity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit pptx

... FragariaxananassaUGT78D1 A. thalianaUGT8 6A1 A. thalianaUGT8 7A1 A. thalianaUGT8 3A1 A. thalianaUGT8 2A1 A. thalianaUGT8 5A1 A. thalianaSbHMNGT S. bicolorUGT76D1 A. thalianaUGT76E1 A. thalianaS39507 ... thalianaOsSGT1 O. sativaUGT74F1 A. thalianaUGT74F2 A. thalianaNtGT2 N. tabacumUGT75C1 A. thalianaUGT75B1 A. thalianaUGT75D1 A. thalianaUGT84B1 A. thalianaUGT8 4A1 A. thalianaFaGT2 FragariaxananassaUGT78D1 ... lycopersicumUGT76F1 A. thalianaCAO69089 V. viniferaUGT76B1 A. thalianaUGT76C1 A. thalianaUGT71B1 A. thalianaCaUGT1 C. roseusUGT71C1 A. thalianaUGT71D2 A. thalianaUGT8 8A1 A. thalianaUGT72E2 A. thalianaUGT72E3...

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