Báo cáo khoa học: Novel dissociation mechanism of a polychaetous annelid extracellular haemoglobin pptx

... Nakagawa T, Kita A, Sasayama Y, Fuku- mori Y & Miki K (2005) Crystallization and prelimin- ary X-ray crystallographic analysis of extracellular giant hemoglobin from pogonophoran Oligobrachia ... microscopy. The analysis of Arenicola haemoglobin dissociation indicates a novel and complex mechanism of dissociation com- pared with other annelid extracellular haemogl...

Ngày tải lên: 23/03/2014, 10:21

... Michigami T, Yamamoto T, Yasui N, Satomura K, Yamagata M, Shima M, Nakajima S, Mushiake S, Okada S & Ozono K (1998) Analysis of localization of mutated tissue-nonspecific alkaline phosphatase ... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al. Novel aggregate f...

Ngày tải lên: 19/02/2014, 17:20

... be a general feature of the a- amylase family. Keywords: amylose; maltodextrin; acarbose; barley a- amy- lase; binding site. a- Amylase is a retaining glycoside hydrolase of family 13 acting on a- 1,4 ... domain B protruding between b-strand 3 and a- helix 3 of the barrel, and a C-terminal, domain C, folded as an antiparallel b-sheet [17–23]. Acarbose is a pseudotetrasaccha...

Ngày tải lên: 08/03/2014, 08:20

... 200–202. 13 Watanabe K, Yoshida R, Shimizu T & Hayaishi O (1985) Enzymatic formation of prostaglandin F2 alpha from prostaglandin H2 and D2. Purification and prop- erties of prostaglandin F synthetase ... prostaglandin F 2a receptor mediated activation of MEK ⁄ ERK-CREB cascade Toshiyuki Ueno and Ko Fujimori Laboratory of Biodefense and Regulation, Osaka University of Pharmaceu...

Ngày tải lên: 28/03/2014, 22:20

... CT ex 114 aa 208 aa 24 aa BamH 34 aa Start Stop Xho1 N -Pro Protease domain Protease domain CT - ex 380 aa 1 II NP 114 aa 208 aa BamH1 34 aa Start Stop Xho1 SS SS G SSS S N-Pro 356 aa III MSTLFII S ILLFLAS F SYAMDI S TIEYKYDKSS AWRTDEEVKEIYELWLAKHDKVY SG LVEYEKRFEIFKDNLKFIDEH NSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCG SCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHG...

Ngày tải lên: 14/02/2014, 14:20

... [46] Fab4-J3 MH2671 cotransformed with pFAB4 and pAN222 (approx. 4–8 copies facB) [46] A6 37 yA2, pabaA1, pdhA1 FGSC, Kansas City, KS, USA A6 34 yA2, pabaA1; pdhB4 FGSC, Kansas City, KS, USA A6 27 yA2, ... exception of MDH a nd citrate synthase and the r esulting absorbance at 340 nm was measured (A 1 ). MDH was added and the absorbance after r eaching t he equilibrium was taken as A...

Ngày tải lên: 19/02/2014, 16:20

... cation radical species in compound I of horseradish peroxidase and catalase. Biochim Biophys Acta 660, 1–7. 5 Kaneko Y, Tamura M & Yamazaki I (1980) Formation of porphyrin pi cation radical ... (1949) The dissociation constant of hydrogen peroxide and the electron affinity of the HO 2 radical. Trans Faraday Soc 45, 224–230. 12 Sugano Y, Muramatsu R, Ichiyanagi A, Sato T & Sh...

Ngày tải lên: 06/03/2014, 00:20

... result of the hierarchical mechanism of lateral assembly. Based on data from [52]. I. V. Baskakov Branched chain mechanism of polymerization FEBS Journal 274 (2007) 3756–3765 ª 2007 The Author ... fibrils. Two parallel processes of fibril elongation and fragmentation occur during the second part of a lag-phase and a subsequent stage that has been referred to as ‘elongation’. A...

Ngày tải lên: 07/03/2014, 10:20

... Benzaldehyde lyase (BAL) catalyzes cleavage and formation of R-benzoin. BAL is known to accept several other substituted aro- matic or aliphatic acyl-acceptors as substrates for the formation of an acyloin ... and catalyse a broad range of reactions mainly involving the cleavage and the formation of C–C-bonds. For instance, they catalyse nonoxidative and oxidative de- carboxylations...

Ngày tải lên: 07/03/2014, 12:20

... residual HMKB is less than 0.64% of the starting concen- tration, indicating an equilibrium constant of at least 150 in favour of 2-acetolactate. When 2-acetolactate was used as the substrate, ... Substrate isomerization and reduction are coordi- nated processes that are conceptually inseparable. While the enzyme can display a separate reductase activity, this should be regarded as a...

Ngày tải lên: 16/03/2014, 18:20