Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc

... 126 2–1 274 ª 2006 The Authors Journal compilation ª 2006 FEBS Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a -phycocyanin lyase and evidence for chromophore binding Kai-Hong ... activities of chromophore binding to all binding sites studied, including a-C84 of PecA [1 6–1 8], by the site-selective effect o...

Ngày tải lên: 23/03/2014, 10:21

... repression in Escherichia coli – a comparison of modelling approaches Andreas Kremling, Sophia Kremling and Katja Bettenbrock Systems Biology Group, Max Planck Institute for Dynamics of Complex ... and mathe- matical models are combined in an attempt to obtain information on the dynamics and regulatory structures of the systems. However, depending on the degree of biol...

Ngày tải lên: 18/02/2014, 13:20

... unusual in having two histidine residues (rather than the His-Asn pair seen in OYE, MR and some of the other member proteins; Fig. 1B) in the active site for ligand and substrate binding. For those ... NaCl contained in 1 L buffer A) to obtain pure protein. Redox potentiometry, ligand binding and stopped-flow kinetic analyses Redox titrations and the determination of...

Ngày tải lên: 20/02/2014, 02:21

... 1. 1–1 .8 1. 1–1 .8 1. 1–1 .8 1. 1–1 .8 1. 1–1 .8 1. 1–1 .8 IRS1 0. 6–0 .7 0. 6–0 .7 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0 PKB 0. 9–1 .1 0. 3–0 .4 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7 Glucose transport 0. 1–0 .2 0.0 2–0 .03 0. 1–0 .2 ... not insulin- stimulated tyrosine phosphorylation of insulin receptor, exhibited insulin resistance. These fin...

Ngày tải lên: 07/03/2014, 16:20

... sequencer and M ALDI-TOF MS. Some peptides were further analysed by ES MS and MS/ MS. Amino acid analysis was performed on a 4151 Alpha Plus amino a cid analyser (LKB Biochrom) using the ninhydrin method. ... loca- tions of histones, with some studies suggesting immunolog- ical roles of histones, in addition to the classical function of histones in chromatin conformation. I...

Ngày tải lên: 08/03/2014, 10:20

... The RmInt1 group II intron has two different retrohoming pathways for mobility using predominantly the nascent lagging strand at DNA replication forks for priming. Nucleic Acids Res 32, 288 0–2 888. 31 ... supporting information is available: Fig. S1. Retrohoming in vivo of wild-type intron and C-terminal region mutant derivatives using RmInt1- engineering constructs DORF. Table...

Ngày tải lên: 15/03/2014, 09:20

... lacking bergaptol and caffeic acid, were run for control and served for background corrections. Linear conditions for kinetic assays were established by adjusting the amount of enzyme protein ... abundances within the first 6 h of elicitation and corresponded to the patterns reported for the elicitor induction of phenylalanine ammonia lyase and 4-coumarate:CoA ligase ac...

Ngày tải lên: 16/03/2014, 16:20

... possible, including the trace of the moved head of Intl. The ChS represents a partial chain for what and the entire chain for did, which moved from its deep structure position to the head of CP, and ... de- fined by allowing non-terminal daughters to dominate a recursive instance of the schema. It is interesting to note that, for phrase structure at least, the relevant...

Ngày tải lên: 18/03/2014, 02:20

... dia- grams of protein–ligand interactions. Protein Eng 8, 12 7–1 34. Supplementary material The following supplementary material is available online: Doc S1. Kinetic characterization of the inhibition of T. ... bound ligand. This allows ‘growing room’ for a catalytic site-bound inhibitor, enabling access to a larger number of target residues and hence increasing the chance of...

Ngày tải lên: 23/03/2014, 07:20

... a lin- ker of two amino acids. The cytochrome b 2 presequence consists of an amino- terminal matrix-targeting sequence (residues 1–3 1) and a sorting sequence (residues 3 2–8 0). Su9-DHFR contains ... method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 24 8–2 54. 109 Darley-Usmar VM (1987) The...

Ngày tải lên: 23/03/2014, 15:21