Báo cáo khoa học: Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP+-dependent malic enzyme docx

... 2006 FEBS 4081 Critical roles of conserved carboxylic acid residues in pigeon cytosolic NADP + -dependent malic enzyme Shuo-Chin Chang 1 *, Kuan-Yu Lin 1 *, Yu-Jung Chen 1 , Chin-Hung Lai 1 , ... examine the contribution of these three amino -acid residues to metal ion binding and enzymatic catalysis. In this study, we sought to delineate the possible roles o...

Ngày tải lên: 23/03/2014, 10:21

... protein. Additionally, spin states of the Fe(III), Fe(II) and Fe(II)-CO complexes of Asp40 mutants were similar to those of the wild-type enzyme in that all mutant proteins were in the six-coordinated ... ncubated for 10 min prior to spectroscopic measurements. Cyanide binding The association rate of cyanide to haem was observed by monitoring changes at 417 nm in spectra of...

Ngày tải lên: 19/02/2014, 16:20

... characteristic of the heme distal side contributes substantially to the ligand binding kinetics of the PAS domain of Ec DOS, as it does in myoglo- bin and hemoglobin. Examination of the effects of these ... phase of the L99F mutant was independent of the O 2 concentration. b Measurement of the rate of O 2 binding was not feasible because of low heme binding affinity and i...

Ngày tải lên: 16/03/2014, 13:20

... longitudinal interactions formed by c-T1 and c-T2. Finally, c-T2 possesses a glycine at position 72 in place of consensus ⁄ c-T1 arginine (Fig. 4B¢). This substitution may ‘open’ the nucleotide-binding ... whereas the level of the c-T1 transcript remained unchanged. Ninety-eight percent of the cells were undergoing mitosis ⁄ cytokinesis at this time (as determined by counting of cel...

Ngày tải lên: 07/03/2014, 04:20

... Src–SH2 binding protein. We observed that MVP interacts with Src in mammalian cells and inhibits the activity of Src tyrosine kinase. Results Isolation of MVP as Src–SH2 interacting protein by GS–SH2 ... ERK activation in Src over- expressing cells. To our knowledge, this is the first report of MVP interact- ing with a protein tyrosine kinase involved in a distinct cell signalling...

Ngày tải lên: 07/03/2014, 12:20

... the processing and the activity of AChE and BChE, notably those involving charged residues, the presence in peptide b of an additional tryptophan (W8) and the presence of six instead of five residues ... counterparts, in agreement with the higher mass of BChE subunits [37]. This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-ter...

Ngày tải lên: 07/03/2014, 03:20

... results in an increased level of free sterol (Fig. 4B), and it would also be expected to increase the cycling of sterols by esterification and hydrolysis, resulting in an increase in intracellular ... complementary roles in maintaining free ergosta-5-enol at appropriate levels, reminiscent of the mechanism reported for cholesterol in adrenal cells ([40,54]). In addition to...

Ngày tải lên: 08/03/2014, 08:20

... heterodimers of subunits ranging from 23 to 30 kDa in size. Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conserved GSH-binding ... arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH-binding or cysteine-binding proteins. The H-site is entirely helical, wi...

Ngày tải lên: 15/03/2014, 09:20

... surprisingly accurately by ENDOR. His365 (the analog amino acid of His354 in X. laevis)is indeed located in the binding pocket nearby the H(1¢) proton. Moreover, His369 (the analog amino acid of His358 ... the protein consists of an a ⁄ b-domain and a FAD-binding domain. The binding pocket, which is smaller but deeper than those of CPD photolyases, is strictly hydrophobic and...

Ngày tải lên: 16/03/2014, 02:20

... proximity of the flavin ring of the FAD cofactor and extends along the substrate-binding domain. Despite the similar overall folding topology in the AOD enzymes, there is significant variability in the size ... is in agreement with the domain organiza- tion in the first line. The third line shows a summary of the identified linear motifs (after filtering processing taking in accoun...

Ngày tải lên: 16/03/2014, 02:20