Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt

... E h7 was linked to an increase in exofacial thiol groups (Fig. 5A, phase 1) and the E h7 ceased to decrease and remained stable when the maximal amount of thiol groups was reached (Fig. 5A, phase ... (Axioplan 2i; Carl Zeiss, Jena, Germany). Images were acquired using axiovision 4.8 software and an AxioCam MRm digital camera (Carl Zeiss). Protein extraction and blotting analys...

Ngày tải lên: 22/03/2014, 21:21

... of a central Pro in membrane-active peptides. To determine the role of Pro in structure, antibiotic activity, and interaction with phospholipids, we generated a ser- ies of model amphipathic a- helical ... liposomes. In addition, biosensor technology indicated that the interaction of PCPs with membranes was predominantly in uenced by initial electrosta...

Ngày tải lên: 23/03/2014, 10:21

... Gagne ´ M & Rappe ´ AK (1998) p-Stacking interactions. Alive and well in proteins. J Biol Chem 273, 15458–15463. 29 Kashiwagi T, Jeanteur D, Haruki M, Katayanagi M, Kanaya S & Morikawa ... extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8, 528–535. 34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E, Nakamura H, Katayanagi K, Morikawa K & Ikeh...

Ngày tải lên: 18/02/2014, 13:20

... functional un- certainty, as defined by Kaplan and Zaenan [3] and Kaplan and Maxwell [2]. In this paper, we relate this characterization to that provided by Tree ~,djoining Grammars (TAG), showing ... level). In [10], we have formal- ized this notion by introducing graph adjoining grammars which generate exactly the same lan- guages as TAGs. In a graph adjoining gramma...

Ngày tải lên: 21/02/2014, 20:20

... 5’-GGAATTCCATATGCAACT CAAAGATGCCAAGCTG)3’ and 5’-CCCAAGCTTGA TACCGCCCAGGCAGAGGTACTTG-3’. The primers used for the PCR cloning of PA0266 were as follows: 5’-GGAATTCCATATGAGCAAGACCAACG AATCCC-3’ and 5’-CCGCTCGAGAGCGAGTTCGTCG AAGCACTCGG-3’. ... Schematic drawing of reactions catalyzed by aminovalerate aminotrans- ferase (PA0266) and glutarate semialdehyde dehydrogenase (PA0265). (B) Ac...

Ngày tải lên: 07/03/2014, 09:20

... investigation in the literature and reaction or pathway databases. Some enzymes may catalyse several reactions, an example being transketolase (EC 2.2.1.1) in the pentose phosphate pathway that catalyses ... speciality chemicals and pharmaceuticals. Moreover, they play a major role for human welfare as agents of biodegradation, spoilage and decay, and some filamentous fungi act...

Ngày tải lên: 17/03/2014, 10:20

... limited to a narrow time window because of rapid pH relaxation to the initial state after the pH jump. We have instead made use of the transformation of a photoreactive species into a stable product ... runs at 2 and 5 mm EDTA gave the same deprotonation rates. Thus, a reaction with EDTA can be neglected, and so can a reaction with OH ) at the pH values used here....

Ngày tải lên: 22/03/2014, 21:20

... belong to the folds ‘NAD(P)- binding Rossmann-fold domain’, ‘FAD ⁄ NAD(P)-binding domain’ and ‘Nucleotide-binding domain’. The dataset was scrutinized and only proteins utilizing FAD or NAD(P) in a ... iter- atively training the two HMMs, one with FAD-binding sequences and one with NAD(P)-binding sequences, using sequences from five of the groups and testing against the rem...

Ngày tải lên: 23/03/2014, 10:21

... receptor has a role in pigmentation and it also mediates the anti -in ammatory action of MSH [11]. Our understanding of the mechanisms of appetite regu- lation and metabolism in mammals is increasing ... and counted with Wallac 1450 (Wizard automatic Microbeta counter). The results were analyzed with a software package suitable for radioligand binding data analysis (...

Ngày tải lên: 23/03/2014, 20:22

... 10. 106 Takaki E, Fujimoto M, Sugahara K, Nakahari T, Yonemura S, Tanaka Y, Hayashida N, Inouye S, Takemoto T, Yamashita H et al. (2006) Maintenance of olfactory neurogenesis requires HSF1, a major ... The Authors Journal compilation ª 2010 FEBS in mammals, the crystallins are aA and aB; bB1, bB2, bB3, bA3 ⁄ A1 and bA4; and cA, cB, cC, cD, cE, cF and cS. In mice, the c-crysta...

Ngày tải lên: 18/02/2014, 04:20