Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf
Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf
... 5’-CACTCTCGAAGAGTTCATA AACGAAGTGAAGAAGAATCTC-3’ 5’-GAGATTCTTCTTCACTTCGT TTATGAACTCTTCGAGAGTG-3’ M. Karlstro ¨ m et al. Structure and thermal stability of T. maritima FEBS Journal 273 (2006) 2851–2868 ª 2006 Karolinska ... [17]. Table 3. Primer sequences. TmIDH mutant Primer sequence R186M 5’-CCTGGAGAAATCGATCA TGAGCTTCGCTCAGTCGTG-3’ 5’-CACGACTGAGCGAAGCT CATGATCGATTTCTCCAGG-3’ F205M 5’-AA...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: The crystal structure of a xyloglucan-specific endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity potx
... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal- ysis of the mode of action of Xgh7 4A has not been performed, Xgh7 4A appears to be an endoglucanase because ... were calcu- lated from a plot of initial reaction rates versus substrate concentration using Prism (GraphPad Software, San Diego, CA, USA). One unit was defined as the...
Ngày tải lên: 23/03/2014, 05:22
Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt
... picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the final fate of the amino acid, i.e. transformation ... shown as sticks; the Zn 2+ ion and the solvent molecule bridging the metal centre to the ligand are shown as magenta and cyan spheres, respectively. S. Garava...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx
... Poznanskaya, A. A., Yakusheva, M.I. & Yakovlev, V .A. (1977) Study of the mechanism of action of adenosylcobalamin-depen- dent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation ... primers 5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢ and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCA TGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTT ACGC...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx
... 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢. 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢. ... for the sharp curvature of the array and reduced superhelical pitch, leading to the formation of a globular structure. Helix a Re...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
... [1–4]. Mammalian annexins A1 [5], A5 , and A 6 [6], as well as plant annexins from Medicago sativa [7] and Arabidop- sis thaliana [8,9], have been implicated in oxidative stress response. In particular, ... annexin Anx24(Ca32), but significant differences are seen when compared to the structure of nonplant annexins. A comparison with the structure of the mamma- lian annexi...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1) pdf
... and catalysis of isomerization for the standard substrate suc-AAPF-pNA. Black and red spectra of the Ala residues in the peptide are obtained from the uncatalyzed and catalyzed reaction, respectively. Acceleration ... biophysical assays. Using a colorimetric coupled assay against succinyl-Ala-Ala-Pro-Phe-p-nit- roaniline (suc-AAPF-pNA), a well-characterized CypA substrate, w...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: The crystal structure of the ring-hydroxylating dioxygenase from Sphingomonas CHY-1 pot
... and catalytic domains of the a subunit. In the heterohexamer, the Rieske domain interacts with the base of the adjacent b subunit and the cata- lytic domain of the adjacent a subunit. Most of the ... and carries the totally conserved amino acids Gly a 354, Glu a 357, Asp a 359 and Asn a 363, which are part of a far-reaching hydrogen network surroundin...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product potx
... ÔdamÕ of the cleft. These four loops of TVA II are superimposed on those of a- amylase (Taka-amylase A) from Aspergillus oryzae (PDB code, 7TAA) [31] and cyclodextrin glucanotransferase (CGTase) from ... into the active cleft compared to Loop II in TVA II (Fig. 5B). In most a- amylases, loop II makes a large bank in the active cleft, as in CGTase and TAA. In contr...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt
... observations. Acta Crystallogr A3 4, 517–525. 34 Vaguine AA, Richelle J & Wodak SJ (1999) sfcheck :a unified set of procedures for evaluating the quality of macromolecular structure- factor data and ... decarboxylated to yield the central interme- diate of ThDP catalysis, the a- carbanion ⁄ enamine (III). Protonation of III yields hydroxyethyl-ThDP (IV), and the release...
Ngày tải lên: 30/03/2014, 10:20