Báo cáo khoa học: A designed curved DNA segment that is a remarkable activator of eukaryotic transcription potx

... designed curved DNA segment that is a remarkable activator of eukaryotic transcription Noriyuki Sumida 1 , Jun-ichi Nishikawa 1 , Haruka Kishi 1 , Miho Amano 1 , Takayo Furuya 1 , Haruyuki Sonobe 1 and ... identify artificial DNA segments that can stably express transgenes in the genome of host cells, we built a series of curved DNA segments that mimic...

Ngày tải lên: 23/03/2014, 10:20

... ligands of the RPTPs in the neuromuscular system. For example, it is known that PTPd and an isoform of LAR can interact homophilically [39] and that LAR can also bind heterophilically to the laminin–nidogen ... predict that an anti- body raised to a sequence outside this domain might have little effect on the RAP assay signal. This was tested with an antibody to nucleolin raised...

Ngày tải lên: 19/02/2014, 05:20

... S., Watanabe ,A. ,Iriguchi,M.,Ishikawa ,A. ,Kawashima,K., Kimura, T., Kishida, Y., Kohara, M., Matsumoto, M., Matsuno, A. , Muraki, A. , Nakazaki, N., Shimpo, S., Sugimoto, M., Takazawa, M., Yamada, M., Yasuda, M. & Tabata, S. (2001) Complete ... domain Fig. 4. Far-UV-CD spectra of NblA (A) , protein concentration dependence of far-UV-CD spectra of NblA (B), thermal denaturation...

Ngày tải lên: 08/03/2014, 10:20

... SNF2-family of DNA- dependent ATPases and is implicated in transcription elongation, transcription coupled repair, and base excision repair. It is a DNA stimula- ted ATPase and remodels chromatin ... the ATPase activity was only detectable after the addition of pUC19 DNA. This indicates that dimerization is not mediated by DNA. Importantly, only residual ATPase activit...

Ngày tải lên: 23/03/2014, 15:20

... An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity Stephanie Ngai, Sarah Batty, Kuo-Chieh Liao and Jeremy Mogridge Department of Laboratory ... inducing vascular leakage that leads to shock and multiorgan failure [3–6]. The role of LeTx in anthrax pathogenesis is complex, however, and probably involves the impairment of the inna...

Ngày tải lên: 16/02/2014, 09:20

... CGA TGG ATA CAG A- 3¢; reverse, 5¢-AGG ACA GTA GAA TTA GGT CAC T-3¢). Knockdown of Hsp10 5a and Hsp105b The double-stranded RNA targeting Hsp105 (Dharmacon; 5¢-GCA AAU CAC UCA UGC AAA CUU-3¢) was ... through signal transducer and activator of transcription- 3 Nobuyuki Yamagishi, Hajime Fujii, Youhei Saito and Takumi Hatayama Department of Biochemistry & Molecular Biology, Divis...

Ngày tải lên: 18/02/2014, 06:20

... approach, data are mapped into a vector space and SLU is performed as a classification problem using Maximal Margin Classifiers (Shawe-Taylor and Cristianini, 2004). Generative models have the advantage ... Trento Italy riccardi@disi.unitn.it Abstract Spoken Language Understanding aims at mapping a natural language spoken sen- tence into a semantic representation. In the last decade t...

Ngày tải lên: 08/03/2014, 21:20

... fibrillogenesis mechanisms that lead to the formation of structural poly- morphism, thanks to its single tryptophan residue and the availability of large quantities at pharmaceutical-grade quality. ... agitation confirm that type B agitated fibrils grow exponentially, and indicate that a small fraction ($ 1in10 5 )of glucagon molecules initiate spontaneous nucleation as soon as glu...

Ngày tải lên: 23/03/2014, 03:20

... generated by PCR ampli- fication of pGST-NES-GFP using the primer pair 5¢-ATA TATGGATCCAGATAAAGATGTGAATGAG-3¢ and 5¢-CGCCCCGACACCCGCCAACACCC-3¢ and Vent DNA polymerase (New England Biolabs, Frankfurt ... signal trans- ducers and activators of transcription (STAT) family of transcription factors are activated at membrane- bound receptors and subsequently translocate into the nucleus...

Ngày tải lên: 30/03/2014, 09:20

... you please elaborate on that specific situa- tion?' Data Analysis The qualitative analysis is based on a phenomenographic approach; that is a qualitative method to use empiric data (e.g., ... veterinarian's perception of the specific farm and by his or her evaluation of the local context. That is, treatment data as an indicator of a certain disease manifestati...

Ngày tải lên: 25/10/2012, 10:45