Báo cáo khoa học: Diversity of human U2AF splicing factors Based on the EMBO Lecture delivered on 7 July 2005 at the 30th FEBS Congress in Budapest pptx

... 305–308. U2AF diversity I. Mollet et al. 4816 FEBS Journal 273 (2006) 48 07 4816 ª 2006 The Authors Journal compilation ª 2006 FEBS THE EMBO LECTURE Diversity of human U2AF splicing factors Based on the EMBO ... domains in relation to U2AF 65 is indicated. (B) The putative functional domains in each protein are aligned with U2AF 35 , and the sim...

Ngày tải lên: 23/03/2014, 10:20

... junction. Confirmation of utilization of exon 1a as a transcription initiation exon and occurrence of alternative splicing To examine whether exon 1a is used as a transcription starting exon in QGP-1 ... or in vitro, including characterization of the promoter region of the gene and the associated transcriptional factors. Therefore, delineation of the transcriptional...

Ngày tải lên: 19/02/2014, 06:20

... partial disulfide bonds. Two conventional methods are often used in the investigation of disulfide bond-containing protein unfolding. One such method is denaturation, which requires the use of denaturants ... vitro. Intermediates are named using the disulfide bonds they contain. Arrows with dashed lines indicate the folding pathway for the first formation of the intra-A disulfi...

Ngày tải lên: 19/02/2014, 12:20

... under control conditions, i.e. with Ca 2+ -free bath solu- tion, upon application of the indicated concentrations of hCaM in the presence of 1 l M free Ca 2+ solution, and after washing off the CaM ... 3A–C) coding for the putative binding sites in hEAG1. In the presence of 25 lm free Ca 2+ , the aspartate- to-alanine mutations in the N-terminal half of CaM (hC...

Ngày tải lên: 07/03/2014, 12:20

... permeabilization induced by the proteins was expressed as a percentage of the maximal permeabiliza- tion obtained at the end of the assay by the addition of Triton X-100 to a final concentration of 2 ... 17. 6 Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of 5 mNÆm )1 ....

Ngày tải lên: 07/03/2014, 17:20

... contribution of the cellular kinases in tissues and is often determined using only one saturating concentration of each substrate. Obviously, choosing a high substrate concentra- tion, as one usually ... is correlated to the presence of the 2¢-OH of the sugar in the cis position. The latter is absent in dCMP and L -3TCMP, and is in trans in the arabinose epimer...

Ngày tải lên: 08/03/2014, 02:20

... a bin- ocular microscope at ·50 magnification. The agglutinating activity is expressed as the minimum agglutinating concen- tration of the sample tested, corresponding to the first con- dition ... al. 171 4 FEBS Journal 277 (2010) 171 3– 172 5 ª 2010 The Authors Journal compilation ª 2010 FEBS in the present study therefore contributes towards elu- cidating the main det...

Ngày tải lên: 22/03/2014, 21:20

... addition, the level of Sp1 is enriched in the Sp1-binding regions of the MLL1 promoter upon exposure to DON. These results demonstrated that Sp1 acts a mediator in translating the effects of DON on ... demonstrated that DON- induced MLL1 and HoxA7 gene upregulation were alleviated in the presence of PP2. These observations demonstrated that Src kinases are involved...

Ngày tải lên: 23/03/2014, 04:21

... as either a or b, based on the number and configuration of the cysteine motifs. The Ônoncoding exonÕ region represents the b-domain region of the transcript that has been truncated by the introduction ... members. Initially, the mutation of a lysine codon to a stop codon in the linker region would have truncated the MT protein after the a-domain. Subsequent tand...

Ngày tải lên: 30/03/2014, 13:20

... tumour cell invasion in vitro by a mechanism involving plasminogen degradation to fragments that limit plasminogen activation and the degradation of laminin. This supports the hypothesis that MMP-3, ... components and activates MMPs resulting in further amplification of BM degradation [ 17 21]. Amongst the MMP family, MMP-3 (stromelysin-1) is considered pivotal to the integrati...

Ngày tải lên: 31/03/2014, 09:20